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G8XQX1

- OXLA_DABRR

UniProt

G8XQX1 - OXLA_DABRR

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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Daboia russelii (Russel's viper) (Vipera russelii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (L-Tyr followed by L-Phe, L-Met, L-Leu, L-Trp), thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Inhibits ADP- and collagen-induced platelet aggregation. This inhibition is inhibited by catalase, indicating the importance of generated H2O2 for the inhibitory effect. This effect on platelets among snake L-amino-acid oxidases is however controversial, since some of them induce aggregation, whereas the other inhibit agonist-induced aggregation. In vivo, this enzyme induces a rapid, substantial and reversible increase in the paw volume of mice (edema). In addition, myofibrosis, and inflammatory cell infiltration on the paw tissue are also observed.1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FADBy similarity

Kineticsi

  1. KM=0.081 mM for L-Tyr (at pH 7.5 and 25 degrees Celsius)1 Publication
  2. KM=0.142 mM for L-Phe (at pH 7.5 and 25 degrees Celsius)1 Publication
  3. KM=0.373 mM for L-Met (at pH 7.5 and 25 degrees Celsius)1 Publication
  4. KM=0.318 mM for L-Trp (at pH 7.5 and 25 degrees Celsius)1 Publication
  5. KM=0.490 mM for L-Leu (at pH 7.5 and 25 degrees Celsius)1 Publication
  6. KM=1.40 mM for L-Ile (at pH 7.5 and 25 degrees Celsius)1 Publication
  7. KM=12.20 mM for L-Arg (at pH 7.5 and 25 degrees Celsius)1 Publication
  8. KM=13.92 mM for L-Val (at pH 7.5 and 25 degrees Celsius)1 Publication
  9. KM=64.00 mM for L-Lys (at pH 7.5 and 25 degrees Celsius)1 Publication
  10. KM=116.48 mM for L-Ala (at pH 7.5 and 25 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891FADBy similarity
Binding sitei108 – 1081SubstrateBy similarity
Binding sitei241 – 2411SubstrateBy similarity
Binding sitei279 – 2791FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei390 – 3901SubstrateBy similarity
Binding sitei475 – 4751FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 622FADBy similarity
Nucleotide bindingi81 – 822FADBy similarity
Nucleotide bindingi105 – 1084FADBy similarity
Nucleotide bindingi482 – 4876FADBy similarity
Nucleotide bindingi482 – 4832SubstrateBy similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: UniProtKB
  2. L-amino-acid oxidase activity Source: UniProtKB
  3. protein dimerization activity Source: UniProtKB

GO - Biological processi

  1. envenomation resulting in induction of edema in other organism Source: UniProtKB
  2. envenomation resulting in negative regulation of platelet aggregation in other organism Source: UniProtKB
  3. hemolysis in other organism Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation inhibiting toxin, Toxin

Keywords - Biological processi

Cytolysis, Hemolysis

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Short name:
DrLAO
Short name:
LAAO
OrganismiDaboia russelii (Russel's viper) (Vipera russelii)
Taxonomic identifieri31159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeDaboia

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. extracellular space of host Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi241 – 2411H → A: Shows high reactivity toward L-Arg, but does not induce change toward L-Leu, L-Phe and L-Met. 1 Publication
Mutagenesisi241 – 2411H → N: No change in activity. 1 Publication
Mutagenesisi241 – 2411H → S: Shows middle reactivity toward L-Arg and L-Phe, but does not induce change toward L-Leu, and L-Met. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 504486L-amino-acid oxidasePRO_5000825648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 191By similarity
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi349 ↔ 430By similarity
Glycosylationi379 – 3791N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer; non-covalently linked.By similarity

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR002937. Amino_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G8XQX1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNVFFMFSLL FLATLGSCAD DKNPLEECFR EDDYEEFLEI AKNGLKKTSN
60 70 80 90 100
PKHIVIVGAG MSGLSAAYVL AGAGHKVTVL EASERPGGRV RTHRNVKEGW
110 120 130 140 150
YANLGPMRVP EKHRIIREYI RKFGLKLNEF VQETENGWYF IKNIRKRVGE
160 170 180 190 200
VKKDPGLLKY PVKPSEAGKS AGQLYQESLG KAVEELKRTN CSYILNKYDT
210 220 230 240 250
YSTKEYLIKE GNLSPGAVDM IGDLLNEDSG YYVSFIESLK HDDIFAYEKR
260 270 280 290 300
FDEIVGGMDQ LPTSMYRAIE ESVHFKARVI KIQQNAEKVT VTYQTTQKNL
310 320 330 340 350
LLETADYVIV CTTSRAARRI TFKPPLPPKK AHALRSVHYR SGTKIFLTCT
360 370 380 390 400
KKFWEDDGIQ GGKSTTDLPS RFIYYPNHNF TTGVGVIIAY GIGDDANFFQ
410 420 430 440 450
ALNLNECADI VFNDLSSIHQ LPKKDLQTFC YPSIIQKWSL DKYAMGAITT
460 470 480 490 500
FTPYQFQHFS EALTAPVGRI FFAGEYTANA HGWIDSTIKS GLTAARDVNR

ASEL
Length:504
Mass (Da):56,888
Last modified:February 22, 2012 - v1
Checksum:i938FBF23BBAA7681
GO

Mass spectrometryi

Molecular mass is 62025 Da from positions 19 - 504. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU663622 mRNA. Translation: ACF70483.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU663622 mRNA. Translation: ACF70483.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002937. Amino_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, characterization and mutagenesis of Russell's viper venom L-amino acid oxidase: insights into its catalytic mechanism."
    Chen H.-S., Wang Y.-M., Huang W.-T., Huang K.-F., Tsai I.-H.
    Biochimie 94:335-344(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-38, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF HIS-241, MASS SPECTROMETRY, IDENTIFICATION BY MASS SPECTROMETRY, 3D-STRUCTURE MODELING IN COMPLEX WITH SUBSTRATE.
    Strain: Eastern India.
    Tissue: Venom and Venom gland.

Entry informationi

Entry nameiOXLA_DABRR
AccessioniPrimary (citable) accession number: G8XQX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2012
Last sequence update: February 22, 2012
Last modified: November 26, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Does not induce dermal hemorrhage when subcutaneously injected into mice at the dose of 40 µg.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3