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G8URN2 (G8URN2_LEGPN) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine--tRNA ligase HAMAP-Rule MF_00176
EC=6.1.1.11 HAMAP-Rule MF_00176
Alternative name(s):
Seryl-tRNA synthetase HAMAP-Rule MF_00176
Seryl-tRNA(Ser/Sec) synthetase HAMAP-Rule MF_00176
Gene names
Name:serS HAMAP-Rule MF_00176
ORF Names:lp12_0517 EMBL AEW50795.1
OrganismLegionella pneumophila subsp. pneumophila ATCC 43290 EMBL AEW50795.1
Taxonomic identifier933093 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella ›

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity. HAMAP-Rule MF_00176

Catalytic activity

ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP-Rule MF_00176

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP-Rule MF_00176

Pathway

Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP-Rule MF_00176

Subunit structure

Homodimer. The tRNA molecule binds across the dimer By similarity. HAMAP-Rule MF_00176

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00176.

Domain

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity. HAMAP-Rule MF_00176

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily. HAMAP-Rule MF_00176

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding262 – 2643ATP By similarity HAMAP-Rule MF_00176
Nucleotide binding349 – 3524ATP By similarity HAMAP-Rule MF_00176
Region231 – 2333Serine binding By similarity HAMAP-Rule MF_00176

Sites

Binding site2851Serine By similarity HAMAP-Rule MF_00176
Binding site3851Serine By similarity HAMAP-Rule MF_00176

Sequences

Sequence LengthMass (Da)Tools
G8URN2 [UniParc].

Last modified February 22, 2012. Version 1.
Checksum: 182ACCFBD9115E8E

FASTA42648,080
        10         20         30         40         50         60 
MLDNQLLREN PQYVATQLLK RGFQFDAVTF SQLEEKRKAL QVSTQSLQNE RNLRSKAIGE 

        70         80         90        100        110        120 
AKSRGENIGP MREEVNKLGA ILEQQKTELD EVLKQIEVIS LSLPNIPHES VPVGKDELDN 

       130        140        150        160        170        180 
QEIRKWGDVP AFSFPVKSHD ELGEALGQMD FALAAKITGS RFVVMKGHLA RLHRALIQFM 

       190        200        210        220        230        240 
LDIHIQQHGY QEIYVPYIVN ADSLLGTGQL PKFEADLFKL TGDNGYYLTS TSEIPVTNTV 

       250        260        270        280        290        300 
REMILSAEQL PIRYVCHSPC FRSEAGSYGK DTKGMIRQHQ FEKVELVWIT KPEDSYNALE 

       310        320        330        340        350        360 
QLTQHAEIIL QRLNLPYRVV ALCTGDIGAG SAKTYDLEVW LPSQNTYREI SSCSNMEAFQ 

       370        380        390        400        410        420 
ARRMKARFRN PDTNEIQLVH TLNGSGLAVG RTLVAIMENY QDEHGNIHIP DALKPYLGGI 


DIISVK

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References

[1]"Whole-Genome Sequence of the Human Pathogen Legionella pneumophila Serogroup 12 Strain 570-CO-H."
Amaro F., Gilbert J.A., Owens S., Trimble W., Shuman H.A.
J. Bacteriol. 194:1613-1614(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 43290 EMBL AEW50795.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003192 Genomic DNA. Translation: AEW50795.1.
RefSeqYP_005184894.1. NC_016811.1.

3D structure databases

ProteinModelPortalG8URN2.
SMRG8URN2. Positions 1-424.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEW50795; AEW50795; lp12_0517.
GeneID11801700.
KEGGlpe:lp12_0517.

Phylogenomic databases

KOK01875.

Enzyme and pathway databases

UniPathwayUPA00906; UER00895.

Family and domain databases

Gene3D1.10.287.40. 1 hit.
HAMAPMF_00176. Ser_tRNA_synth_type1.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERPTHR11778. PTHR11778. 1 hit.
PfamPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSPR00981. TRNASYNTHSER.
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMsTIGR00414. serS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG8URN2_LEGPN
AccessionPrimary (citable) accession number: G8URN2
Entry history
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: April 3, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info