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G8UIG9

- G8UIG9_TANFA

UniProt

G8UIG9 - G8UIG9_TANFA

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Tannerella forsythia (strain ATCC 43037 / JCM 10827 / FDC 338) (Bacteroides forsythus)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 17 (01 Oct 2014)
      Sequence version 1 (22 Feb 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei24 – 241ATP; via amide nitrogenUniRule annotation
    Metal bindingi116 – 1161Magnesium; catalyticUniRule annotation
    Active sitei141 – 1411Proton acceptorUniRule annotation
    Binding sitei168 – 1681Allosteric activator ADPUniRule annotation
    Binding sitei176 – 1761Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei225 – 2251Allosteric activator ADPUniRule annotation
    Binding sitei236 – 2361SubstrateUniRule annotation
    Binding sitei260 – 2601Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi85 – 862ATPUniRule annotation
    Nucleotide bindingi115 – 1184ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTFOR203275:GHRQ-1155-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotationImported
    Ordered Locus Names:BFO_1157Imported
    OrganismiTannerella forsythia (strain ATCC 43037 / JCM 10827 / FDC 338) (Bacteroides forsythus)Imported
    Taxonomic identifieri203275 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaeTannerella
    ProteomesiUP000005436: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni34 – 385Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni139 – 1413Substrate bindingUniRule annotation
    Regioni183 – 1853Substrate bindingUniRule annotation
    Regioni199 – 2013Allosteric activator ADP bindingUniRule annotation
    Regioni227 – 2293Allosteric activator ADP bindingUniRule annotation
    Regioni266 – 2694Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    KOiK00850.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    G8UIG9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFKLIRSNM STKIKCIGLL TSGGDAPGMN AAIRAVTRTA IYNGMAVKGI    50
    YRGYKGLITN EIEDFKTQSV SNIIQRGGTI LKTARCAEFQ TSEGRKLAYD 100
    VILQHGINAM IVIGGDGSLR GARDFAQEYD FPIIGLPGTI DNDLNGTDTT 150
    IGYDTALNTI MECVDKIRDT ASSHERLFFV EVMGRDAGFL ALNGAIASGA 200
    EAAIIPEISL KKDQLAEMIE MGFRKSKNSS IVLVAESEVT GGAMGVAERV 250
    KKQYPGFDVR VSILGHLQRG GSPTAQDRIL STRMGVAAVD ALIEGQRNAM 300
    VGIQNEELTL VPFVKAIKKD KPINRDLLNV LRIVST 336
    Length:336
    Mass (Da):36,204
    Last modified:February 22, 2012 - v1
    Checksum:iD005340F009D9E0B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003191 Genomic DNA. Translation: AEW22384.1.
    RefSeqiYP_005014063.1. NC_016610.1.

    Genome annotation databases

    EnsemblBacteriaiAEW22384; AEW22384; BFO_1157.
    GeneIDi11658166.
    KEGGitfo:BFO_1157.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003191 Genomic DNA. Translation: AEW22384.1 .
    RefSeqi YP_005014063.1. NC_016610.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEW22384 ; AEW22384 ; BFO_1157 .
    GeneIDi 11658166.
    KEGGi tfo:BFO_1157.

    Phylogenomic databases

    KOi K00850.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci TFOR203275:GHRQ-1155-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Tannerella forsythia ATCC 43037."
      Dewhirst F., Tanner A., Izard J., Brinkac L., Durkin A.S., Hostetler J., Shetty J., Torralba M., Gill S., Nelson K.
      Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43037 / JCM 10827 / FDC 338Imported.

    Entry informationi

    Entry nameiG8UIG9_TANFA
    AccessioniPrimary (citable) accession number: G8UIG9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 22, 2012
    Last sequence update: February 22, 2012
    Last modified: October 1, 2014
    This is version 17 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3