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G8TGD7

- G8TGD7_NIAKG

UniProt

G8TGD7 - G8TGD7_NIAKG

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Niastella koreensis (strain DSM 17620 / KACC 11465 / GR20-10)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 16 (01 Oct 2014)
      Sequence version 1 (22 Feb 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
    Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
    Active sitei132 – 1321Proton acceptorUniRule annotation
    Binding sitei159 – 1591Allosteric activator ADPUniRule annotation
    Binding sitei167 – 1671Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei216 – 2161Allosteric activator ADPUniRule annotation
    Binding sitei227 – 2271SubstrateUniRule annotation
    Binding sitei251 – 2511Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi76 – 772ATPUniRule annotation
    Nucleotide bindingi106 – 1094ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciNKOR700598:GHC3-996-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:Niako_0981Imported
    OrganismiNiastella koreensis (strain DSM 17620 / KACC 11465 / GR20-10)Imported
    Taxonomic identifieri700598 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesSphingobacteriiaSphingobacterialesChitinophagaceaeNiastella
    ProteomesiUP000005438: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 295Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni130 – 1323Substrate bindingUniRule annotation
    Regioni174 – 1763Substrate bindingUniRule annotation
    Regioni190 – 1923Allosteric activator ADP bindingUniRule annotation
    Regioni218 – 2203Allosteric activator ADP bindingUniRule annotation
    Regioni257 – 2604Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    KOiK00850.
    OMAiGFGGRCV.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    G8TGD7-1 [UniParc]FASTAAdd to Basket

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    MAKKVTKIGV LTSGGDSPGM NAAIRAVVRT GNYYGLEVFG IMRGYSGIID    50
    NDIVPMHSRS VANIIQRGGT ILKTARCKEF FTPEGRLKAY NNLKKLGIDG 100
    LVIIGGDGSF RGADIFSREF DIPCIGLPGT IDKDIAGTDF TIGFDTAVNT 150
    AVEAIDKIRD TADAHDRLFI IEVMGRDAGY IALHSGIATG AEHILIPERK 200
    TDIEELVASL QEKERRKKLV NMVVVAEGDE FGGANEVAKV VKERLPTTDT 250
    RVCILGHIQR GGSPTCLDRL IASRMGYAAV ESLLEGRHNV MVGILNNRMH 300
    YTLLERAVKS KQKISDEWIK IVKILAS 327
    Length:327
    Mass (Da):35,611
    Last modified:February 22, 2012 - v1
    Checksum:iB9021465E65CCEF7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003178 Genomic DNA. Translation: AEV97360.1.
    RefSeqiWP_014217274.1. NC_016609.1.
    YP_005006764.1. NC_016609.1.

    Genome annotation databases

    EnsemblBacteriaiAEV97360; AEV97360; Niako_0981.
    GeneIDi11652727.
    KEGGinko:Niako_0981.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003178 Genomic DNA. Translation: AEV97360.1 .
    RefSeqi WP_014217274.1. NC_016609.1.
    YP_005006764.1. NC_016609.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEV97360 ; AEV97360 ; Niako_0981 .
    GeneIDi 11652727.
    KEGGi nko:Niako_0981.

    Phylogenomic databases

    KOi K00850.
    OMAi GFGGRCV.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci NKOR700598:GHC3-996-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17620 / KACC 11465 / GR20-10Imported.

    Entry informationi

    Entry nameiG8TGD7_NIAKG
    AccessioniPrimary (citable) accession number: G8TGD7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 22, 2012
    Last sequence update: February 22, 2012
    Last modified: October 1, 2014
    This is version 16 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3