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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Niastella koreensis (strain DSM 17620 / KACC 11465 / GR20-10)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
Active sitei132 – 1321Proton acceptorUniRule annotation
Binding sitei159 – 1591Allosteric activator ADPUniRule annotation
Binding sitei167 – 1671Substrate; shared with dimeric partnerUniRule annotation
Binding sitei216 – 2161Allosteric activator ADPUniRule annotation
Binding sitei227 – 2271SubstrateUniRule annotation
Binding sitei251 – 2511Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 772ATPUniRule annotation
Nucleotide bindingi106 – 1094ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciNKOR700598:GHC3-996-MONOMER.
UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Niako_0981Imported
OrganismiNiastella koreensis (strain DSM 17620 / KACC 11465 / GR20-10)Imported
Taxonomic identifieri700598 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesSphingobacteriiaSphingobacterialesChitinophagaceaeNiastella
ProteomesiUP000005438 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 295Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni130 – 1323Substrate bindingUniRule annotation
Regioni174 – 1763Substrate bindingUniRule annotation
Regioni190 – 1923Allosteric activator ADP bindingUniRule annotation
Regioni218 – 2203Allosteric activator ADP bindingUniRule annotation
Regioni257 – 2604Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.
OMAiIVIVTES.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G8TGD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKVTKIGV LTSGGDSPGM NAAIRAVVRT GNYYGLEVFG IMRGYSGIID
60 70 80 90 100
NDIVPMHSRS VANIIQRGGT ILKTARCKEF FTPEGRLKAY NNLKKLGIDG
110 120 130 140 150
LVIIGGDGSF RGADIFSREF DIPCIGLPGT IDKDIAGTDF TIGFDTAVNT
160 170 180 190 200
AVEAIDKIRD TADAHDRLFI IEVMGRDAGY IALHSGIATG AEHILIPERK
210 220 230 240 250
TDIEELVASL QEKERRKKLV NMVVVAEGDE FGGANEVAKV VKERLPTTDT
260 270 280 290 300
RVCILGHIQR GGSPTCLDRL IASRMGYAAV ESLLEGRHNV MVGILNNRMH
310 320
YTLLERAVKS KQKISDEWIK IVKILAS
Length:327
Mass (Da):35,611
Last modified:February 22, 2012 - v1
Checksum:iB9021465E65CCEF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003178 Genomic DNA. Translation: AEV97360.1.
RefSeqiWP_014217274.1. NC_016609.1.
YP_005006764.1. NC_016609.1.

Genome annotation databases

EnsemblBacteriaiAEV97360; AEV97360; Niako_0981.
KEGGinko:Niako_0981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003178 Genomic DNA. Translation: AEV97360.1.
RefSeqiWP_014217274.1. NC_016609.1.
YP_005006764.1. NC_016609.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEV97360; AEV97360; Niako_0981.
KEGGinko:Niako_0981.

Phylogenomic databases

KOiK00850.
OMAiIVIVTES.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.
BioCyciNKOR700598:GHC3-996-MONOMER.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17620 / KACC 11465 / GR20-10Imported.

Entry informationi

Entry nameiG8TGD7_NIAKG
AccessioniPrimary (citable) accession number: G8TGD7
Entry historyi
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: April 29, 2015
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.