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G8TGD7 (G8TGD7_NIAKG) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339

Short name=ATP-PFK HAMAP-Rule MF_00339
Short name=Phosphofructokinase HAMAP-Rule MF_00339
EC=2.7.1.11 HAMAP-Rule MF_00339
Alternative name(s):
Phosphohexokinase HAMAP-Rule MF_00339
Gene names
Name:pfkA HAMAP-Rule MF_00339
Ordered Locus Names:Niako_0981 EMBL AEV97360.1
OrganismNiastella koreensis (strain DSM 17620 / KACC 11465 / GR20-10) [Complete proteome] [HAMAP] EMBL AEV97360.1
Taxonomic identifier700598 [NCBI]
Taxonomic lineageBacteriaBacteroidetesSphingobacteriiaSphingobacterialesChitinophagaceaeNiastella

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_00339

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339 SAAS SAAS022953

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339 SAAS SAAS012828

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity SAAS SAAS022953 HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily. HAMAP-Rule MF_00339

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding76 – 772ATP By similarity HAMAP-Rule MF_00339
Nucleotide binding106 – 1094ATP By similarity HAMAP-Rule MF_00339
Region25 – 295Allosteric activator ADP binding; shared with dimeric partner By similarity HAMAP-Rule MF_00339
Region130 – 1323Substrate binding By similarity HAMAP-Rule MF_00339
Region174 – 1763Substrate binding By similarity HAMAP-Rule MF_00339
Region190 – 1923Allosteric activator ADP binding By similarity HAMAP-Rule MF_00339
Region218 – 2203Allosteric activator ADP binding By similarity HAMAP-Rule MF_00339
Region257 – 2604Substrate binding By similarity HAMAP-Rule MF_00339

Sites

Active site1321Proton acceptor By similarity HAMAP-Rule MF_00339
Metal binding1071Magnesium; catalytic By similarity HAMAP-Rule MF_00339
Binding site151ATP; via amide nitrogen By similarity HAMAP-Rule MF_00339
Binding site1591Allosteric activator ADP By similarity HAMAP-Rule MF_00339
Binding site1671Substrate; shared with dimeric partner By similarity HAMAP-Rule MF_00339
Binding site2161Allosteric activator ADP By similarity HAMAP-Rule MF_00339
Binding site2271Substrate By similarity HAMAP-Rule MF_00339
Binding site2511Substrate; shared with dimeric partner By similarity HAMAP-Rule MF_00339

Sequences

Sequence LengthMass (Da)Tools
G8TGD7 [UniParc].

Last modified February 22, 2012. Version 1.
Checksum: B9021465E65CCEF7

FASTA32735,611
        10         20         30         40         50         60 
MAKKVTKIGV LTSGGDSPGM NAAIRAVVRT GNYYGLEVFG IMRGYSGIID NDIVPMHSRS 

        70         80         90        100        110        120 
VANIIQRGGT ILKTARCKEF FTPEGRLKAY NNLKKLGIDG LVIIGGDGSF RGADIFSREF 

       130        140        150        160        170        180 
DIPCIGLPGT IDKDIAGTDF TIGFDTAVNT AVEAIDKIRD TADAHDRLFI IEVMGRDAGY 

       190        200        210        220        230        240 
IALHSGIATG AEHILIPERK TDIEELVASL QEKERRKKLV NMVVVAEGDE FGGANEVAKV 

       250        260        270        280        290        300 
VKERLPTTDT RVCILGHIQR GGSPTCLDRL IASRMGYAAV ESLLEGRHNV MVGILNNRMH 

       310        320 
YTLLERAVKS KQKISDEWIK IVKILAS 

« Hide

References

[1]"The complete genome of Niastella koreensis GR20-10."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Han J., Lapidus A., Bruce D., Goodwin L., Pitluck S., Peters L., Kyrpides N., Mavromatis K., Ivanova N., Mikhailova N., Davenport K., Saunders E., Detter J.C., Tapia R., Han C., Land M., Hauser L. expand/collapse author list , Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Tindall B., Pomrenke H., Brambilla E., Klenk H.-P., Eisen J.A.
Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17620 / KACC 11465 / GR20-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003178 Genomic DNA. Translation: AEV97360.1.
RefSeqYP_005006764.1. NC_016609.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEV97360; AEV97360; Niako_0981.
GeneID11652727.
KEGGnko:Niako_0981.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00850.
OMAGFGGRCV.

Enzyme and pathway databases

BioCycNKOR700598:GHC3-996-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG8TGD7_NIAKG
AccessionPrimary (citable) accession number: G8TGD7
Entry history
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: July 9, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)