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G8SL09 (G8SL09_ACTS5) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AEV88214.1
Ordered Locus Names:ACPL_7334 EMBL AEV88214.1
OrganismActinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110) [Complete proteome] [HAMAP] EMBL AEV88214.1
Taxonomic identifier134676 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeActinoplanes

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain155 – 357203ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding182 – 23756ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3111Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3241Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3241Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3261Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G8SL09 [UniParc].

Last modified February 22, 2012. Version 1.
Checksum: 20B37C98E3A31312

FASTA36738,657
        10         20         30         40         50         60 
MTTPRKTRVA IVFGGRSTEH AISCVSAGAI LGALDPDQYE TVPVGITREG RWVLAAGDPA 

        70         80         90        100        110        120 
QLAIAGERLP EITAGSGSAV VLPADPTAAE LIVRDPADGV AELAELAGVD VVFPVLHGAY 

       130        140        150        160        170        180 
GEDGTIQGML EMAGIPYVGA NVFASAAAMD KEFTKKLAAA EGIPVGPYAV LRAGMSLTEA 

       190        200        210        220        230        240 
DKERLGLPVF VKPSRAGSST GITKVTDWAD LDAAITKARE IDPKVLVEAA IVGSEIECGV 

       250        260        270        280        290        300 
LEGEFGGPPQ ASLLAEIHMD GADWYDFATK YLVGSRYTIP AALPHDLTRQ VQEYALRTFT 

       310        320        330        340        350        360 
ALDCAGLARV DFFVTAAGEV YLNEINTMPG MTPTSMFPQM WAATGLEYPK VVDRLIRTAL 


RRGTGLH 

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References

[1]"The complete genome sequence of the acarbose producer Actinoplanes sp. SE50/110."
Schwientek P., Szczepanowski R., Kalinowski J., Klein A., Selber K., Wehmeier U.F., Stoye J., Puehler A.
Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31044 / CBS 674.73 / SE50/110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003170 Genomic DNA. Translation: AEV88214.1.
RefSeqYP_006270282.1. NC_017803.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEV88214; AEV88214; ACPL_7334.
GeneID12824889.
KEGGase:ACPL_7334.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.
OMAMDKIAMK.

Enzyme and pathway databases

BioCycASP134676:GL7H-7318-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG8SL09_ACTS5
AccessionPrimary (citable) accession number: G8SL09
Entry history
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: February 19, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)