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G8SIJ9 (G8SIJ9_ACTS5) Unreviewed, UniProtKB/TrEMBL

Last modified October 16, 2013. Version 11. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108

EC=1.1.1.42 PIRNR PIRNR000108
Gene names
Name:icdA EMBL AEV81797.1
Ordered Locus Names:ACPL_900 EMBL AEV81797.1
OrganismActinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110) [Complete proteome] [HAMAP] EMBL AEV81797.1
Taxonomic identifier134676 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeActinoplanes

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding75 – 773NADP By similarity PIRSR PIRSR000108-4
Nucleotide binding309 – 3146NADP By similarity PIRSR PIRSR000108-4
Region94 – 1007Substrate binding By similarity

Sites

Metal binding2511Magnesium or manganese By similarity PIRSR PIRSR000108-3
Metal binding2741Magnesium or manganese By similarity PIRSR PIRSR000108-3
Binding site771Substrate By similarity
Binding site821NADP By similarity PIRSR PIRSR000108-4
Binding site1091Substrate By similarity
Binding site1321Substrate By similarity
Binding site2591NADP By similarity PIRSR PIRSR000108-4
Binding site3271NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
Site1391Critical for catalysis By similarity PIRSR PIRSR000108-1
Site2111Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

Sequence LengthMass (Da)Tools
G8SIJ9 [UniParc].

Last modified February 22, 2012. Version 1.
Checksum: 59E08A6AD06C89AE

FASTA40445,116
        10         20         30         40         50         60 
MAKIKVKNPV VEIDGDEMTR IIWKQIREQL IQPYLDVNLE YYDLGIQYRD ETDDQVTIDA 

        70         80         90        100        110        120 
ANAIKRHGVG VKCATITPDE ARVQEFGLKK MWRSPNGTIR NILGGVVFRE PIIMKNVPRL 

       130        140        150        160        170        180 
VPGWTKPIVI GRHAHGDQYK ASDFVAPSKG KMTVTFTPED GSAPIEMHVA DFPAGGVGMA 

       190        200        210        220        230        240 
MYNFDESIRD FARASLRYGL ARNYPVYLST KNTILKAYDG RFKDLFAEIF ETEFADQFKE 

       250        260        270        280        290        300 
AGITYEHRLI DDMVAAALKW EGGYVWACKN YDGDVQSDTV AQGFGSLGLM TSVLMTPDGQ 

       310        320        330        340        350        360 
TVEAEAAHGT VTRHYRQWQK GEKTSTNPIA SIFAWTGGLK HRGKLDGTPE VTQFAEALEQ 

       370        380        390        400 
VCIETVEGGQ MTKDLALLIS KDAPWLSTDE FMNALDENLA RKLA 

« Hide

References

[1]"The complete genome sequence of the acarbose producer Actinoplanes sp. SE50/110."
Schwientek P., Szczepanowski R., Kalinowski J., Klein A., Selber K., Wehmeier U.F., Stoye J., Puehler A.
Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 31044 / CBS 674.73 / SE50/110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003170 Genomic DNA. Translation: AEV81797.1.
RefSeqYP_006263865.1. NC_017803.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEV81797; AEV81797; ACPL_900.
GeneID12821715.
KEGGase:ACPL_900.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00031.
OMAQHQQGKP.

Enzyme and pathway databases

BioCycASP134676:GL7H-806-MONOMER.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG8SIJ9_ACTS5
AccessionPrimary (citable) accession number: G8SIJ9
Entry history
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: October 16, 2013
This is version 11 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)