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Protein

Isocitrate dehydrogenase [NADP]

Gene

icdA

Organism
Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771SubstrateUniRule annotation
Binding sitei82 – 821NADPUniRule annotation
Binding sitei109 – 1091SubstrateUniRule annotation
Binding sitei132 – 1321SubstrateUniRule annotation
Sitei139 – 1391Critical for catalysisUniRule annotation
Sitei211 – 2111Critical for catalysisUniRule annotation
Metal bindingi251 – 2511Magnesium or manganeseUniRule annotation
Binding sitei259 – 2591NADPUniRule annotation
Metal bindingi274 – 2741Magnesium or manganeseUniRule annotation
Binding sitei327 – 3271NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 773NADPUniRule annotation
Nucleotide bindingi309 – 3146NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationImported

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

BioCyciASP134676:GL7H-806-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Name:icdAImported
Ordered Locus Names:ACPL_900Imported
OrganismiActinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110)Imported
Taxonomic identifieri134676 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeActinoplanes
ProteomesiUP000005440 Componenti: Chromosome

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 1007Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

KOiK00031.
OMAiDVMSDMI.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G8SIJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKIKVKNPV VEIDGDEMTR IIWKQIREQL IQPYLDVNLE YYDLGIQYRD
60 70 80 90 100
ETDDQVTIDA ANAIKRHGVG VKCATITPDE ARVQEFGLKK MWRSPNGTIR
110 120 130 140 150
NILGGVVFRE PIIMKNVPRL VPGWTKPIVI GRHAHGDQYK ASDFVAPSKG
160 170 180 190 200
KMTVTFTPED GSAPIEMHVA DFPAGGVGMA MYNFDESIRD FARASLRYGL
210 220 230 240 250
ARNYPVYLST KNTILKAYDG RFKDLFAEIF ETEFADQFKE AGITYEHRLI
260 270 280 290 300
DDMVAAALKW EGGYVWACKN YDGDVQSDTV AQGFGSLGLM TSVLMTPDGQ
310 320 330 340 350
TVEAEAAHGT VTRHYRQWQK GEKTSTNPIA SIFAWTGGLK HRGKLDGTPE
360 370 380 390 400
VTQFAEALEQ VCIETVEGGQ MTKDLALLIS KDAPWLSTDE FMNALDENLA

RKLA
Length:404
Mass (Da):45,116
Last modified:February 22, 2012 - v1
Checksum:i59E08A6AD06C89AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003170 Genomic DNA. Translation: AEV81797.1.
RefSeqiWP_014687870.1. NC_017803.1.
YP_006263865.1. NC_017803.1.

Genome annotation databases

EnsemblBacteriaiAEV81797; AEV81797; ACPL_900.
KEGGiase:ACPL_900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003170 Genomic DNA. Translation: AEV81797.1.
RefSeqiWP_014687870.1. NC_017803.1.
YP_006263865.1. NC_017803.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEV81797; AEV81797; ACPL_900.
KEGGiase:ACPL_900.

Phylogenomic databases

KOiK00031.
OMAiDVMSDMI.

Enzyme and pathway databases

BioCyciASP134676:GL7H-806-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of the acarbose producer Actinoplanes sp. SE50/110."
    Schwientek P., Szczepanowski R., Kalinowski J., Klein A., Selber K., Wehmeier U.F., Stoye J., Puehler A.
    Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 31044 / CBS 674.73 / SE50/110Imported.

Entry informationi

Entry nameiG8SIJ9_ACTS5
AccessioniPrimary (citable) accession number: G8SIJ9
Entry historyi
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: April 29, 2015
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.