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G8RQ29 (G8RQ29_MYCRN) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 10. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UvrABC system protein B HAMAP-Rule MF_00204
Short name=Protein UvrB HAMAP-Rule MF_00204
Alternative name(s):
Excinuclease ABC subunit B HAMAP-Rule MF_00204
Gene names
Name:uvrB HAMAP-Rule MF_00204
Ordered Locus Names:MycrhN_4615 EMBL AEV75102.1
OrganismMycobacterium rhodesiae (strain NBB3) [Complete proteome] [HAMAP]
Taxonomic identifier710685 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium ›

Protein attributes

Sequence length716 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204 SAAS SAAS024759

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity. HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family. HAMAP-Rule MF_00204 RuleBase RU003587

Contains 1 UVR domain. HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS024759

Contains 1 helicase ATP-binding domain. HAMAP-Rule MF_00204 SAAS SAAS024759

Contains 1 helicase C-terminal domain. HAMAP-Rule MF_00204 SAAS SAAS024759

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain49 – 201153Helicase ATP-binding By similarity HAMAP-Rule MF_00204
Domain453 – 606154Helicase C-terminal By similarity HAMAP-Rule MF_00204
Domain671 – 70636UVR By similarity HAMAP-Rule MF_00204
Nucleotide binding62 – 698ATP By similarity HAMAP-Rule MF_00204
Motif115 – 13824Beta-hairpin By similarity HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
G8RQ29 [UniParc].

Last modified February 22, 2012. Version 1.
Checksum: FF90138A702B3C37

FASTA71680,675
        10         20         30         40         50         60 
MAFATEHPVL AHSEYRPVDA IVRTGGRFEV VSEHQPAGDQ PTAIQELERR ISAGERDVVL 

        70         80         90        100        110        120 
LGATGTGKSA TTAWLIERLQ RPTLVMAPNK TLAAQLANEL REMLPHNAVE YFVSYYDYYQ 

       130        140        150        160        170        180 
PEAYIAQTDT YIEKDSSIND DVERLRHSAT SSLLSRRDVV VVASVSCIYG LGTPQSYLDR 

       190        200        210        220        230        240 
SVELQVGQEV PRDALLRLLV DVQYTRNDMS FTRGSFRVRG DTVEIIPSYE ELAVRIEFFG 

       250        260        270        280        290        300 
DEIEALYYMH PLTGDVVRQV DSLRVFPATH YVAGPERMAH AISTIEQELE ERLAELEGQG 

       310        320        330        340        350        360 
KLLEAQRLRM RTNYDVEMMR QVGFCSGIEN YSRHIDARPA GSAPATLLDY FPEDFLLVID 

       370        380        390        400        410        420 
ESHVTVPQIG GMYEGDMSRK RNLVDFGFRL PSAVDNRPLT WEEFADRIGQ TVYLSATPGP 

       430        440        450        460        470        480 
YELSQSGGEF VEQVIRPTGL VDPQVVLKPT KGQIDDLIGE IRKRTERDER VLVTTLTKKM 

       490        500        510        520        530        540 
AEDLTDYLLE MGIRVRYLHS EVDTLRRVEL LRQLRLGDYD VLVGINLLRE GLDLPEVSLV 

       550        560        570        580        590        600 
SILDADKEGF LRSTRSLIQT IGRAARNVSG EVHMYADKIT DSMKEAIDET DRRRAKQIAY 

       610        620        630        640        650        660 
NEEHGIDPKP LRKKIADILD QVYREADDVE VGGSGRNASR GRRAQGEPGR AVSAGIIEGR 

       670        680        690        700        710 
DTTNMPRAEL ADLIRDLTEQ MMTAARDLQF ELAARIRDEI HDLKKELRGM DAAGLK

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References

[1]"Complete sequence of Mycobacterium rhodesiae NBB3."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Mikhailova N., Gu W., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Mattes T. expand/collapse author list , Holmes A., Rutledge P., Paulsen I., Coleman N., Woyke T.
Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBB3 EMBL AEV75102.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003169 Genomic DNA. Translation: AEV75102.1.
RefSeqYP_005002317.1. NC_016604.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEV75102; AEV75102; MycrhN_4615.
GeneID11641971.
KEGGmrh:MycrhN_4615.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03702.

Family and domain databases

Gene3D4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF46600. UvrB_C. 1 hit.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG8RQ29_MYCRN
AccessionPrimary (citable) accession number: G8RQ29
Entry history
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: April 3, 2013
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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