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G8PCJ1 (G8PCJ1_PEDCP) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:PECL_684 EMBL AEV94976.1
OrganismPediococcus claussenii (strain ATCC BAA-344 / DSM 14800 / JCM 18046 / KCTC 3811 / P06) [Complete proteome] [HAMAP] EMBL AEV94976.1
Taxonomic identifier701521 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaePediococcus

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain146 – 340195ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding173 – 22654ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2951Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3071Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3071Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3091Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G8PCJ1 [UniParc].

Last modified February 22, 2012. Version 1.
Checksum: E98EC2EAA964F944

FASTA35138,349
        10         20         30         40         50         60 
MKIAVLAGGK STERNVSLSS GSKITNALRA KGHQATMIDL FLGYELGEQE SIEDVFEHSN 

        70         80         90        100        110        120 
TTTDYEISDE VLTDAAIEKL RTDGTVGLFG KNVLDIARAS DIVFLALHGG DGENGKIQAV 

       130        140        150        160        170        180 
LDLNNIRYTG SGTLAAGMAM DKAISKEIML FNGIKTARFA IMKADDEEAP ELDFGYPMVV 

       190        200        210        220        230        240 
KPNSGGSSVG AMIVQNDQEF KSALQEGFKF DEELIIEEFV SGREFSLGVV NGYALPAIEI 

       250        260        270        280        290        300 
VVNDGWYDYE HKFQTGSTTQ FITPPDIDDD VHNEMKRLSV ATMEALQMEN YGRIDFLVND 

       310        320        330        340        350 
SGVYVIEANT LPGMTPLSLM PQEAEADGLS YEDLCDQIVA GKMKIYEDRA K 

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References

[1]"Genome and plasmid sequences for the beer-spoilage organism Pediococcus claussenii ATCC BAA-344T."
Pittet V., Abegunde T., Marfleet T., Haakensen M., Morrow K., Jayaprakash T., Schroeder K., Trost B., Byrns S., Bergsveinson J., Kusalik A., Ziola B.
Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-344 / DSM 14800 / JCM 18046 / KCTC 3811 / P06.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003137 Genomic DNA. Translation: AEV94976.1.
RefSeqYP_005004652.1. NC_016605.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEV94976; AEV94976; PECL_684.
GeneID11646852.
KEGGpce:PECL_684.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.
OMAKYTEGFA.

Enzyme and pathway databases

BioCycPCLA701521:GKFD-665-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG8PCJ1_PEDCP
AccessionPrimary (citable) accession number: G8PCJ1
Entry history
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: February 19, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)