Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

G8P5E5 (G8P5E5_LACLC) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:llh_2000 EMBL AEU39576.1
OrganismLactococcus lactis subsp. cremoris A76 [Complete proteome] EMBL AEU39576.1
Taxonomic identifier1104322 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain150 – 353204ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding180 – 23556ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3071Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3201Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3201Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3221Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G8P5E5 [UniParc].

Last modified February 22, 2012. Version 1.
Checksum: 9CA871B394FD640A

FASTA36740,568
        10         20         30         40         50         60 
MVSKKEATDK AVSKKGRNMS KETLILLYGG RSAEREVSVL SAESVMRAVN YSRFIVKTYF 

        70         80         90        100        110        120 
ITKGGEFIKT QEFTDTPAEE EKLLTNDLAE SYPKISPAAI YEKDAVVFPV LHGPMGEDGS 

       130        140        150        160        170        180 
IQGFLEILRL AYVGPNILSA SSTMDKLLAK HVFEAVGVPQ VPYVAAFADE NQAEIAQEVV 

       190        200        210        220        230        240 
EKLEFPVFVK PANMGSSVGI SKVDDLADLQ PALSEAYKYD NRVVIEQGVD AREIECAVLG 

       250        260        270        280        290        300 
NNSDVSATLP GEVVKDVGFY DYNSKYIDNK IQMDIPAKVS ADLAQKIQEY AKKAYKAVNG 

       310        320        330        340        350        360 
AGLSRCDFFV TKDGNVYLNE VNAIPGFTQW SMYPLLWENM GLSYSDLIEK LVDLAKETFE 


TRENHLL 

« Hide

References

[1]"Complete Genome Sequence of Lactococcus lactis subsp. cremoris A76."
Bolotin A., Quinquis B., Ehrlich S.D., Sorokin A.
Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: A76 EMBL AEU39576.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003132 Genomic DNA. Translation: AEU39576.1.
RefSeqYP_005874999.1. NC_017492.1.

3D structure databases

ProteinModelPortalG8P5E5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEU39576; AEU39576; llh_2000.
GeneID12483971.
KEGGllr:llh_2000.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycLLAC1104322:GLFE-415-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG8P5E5_LACLC
AccessionPrimary (citable) accession number: G8P5E5
Entry history
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: June 11, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)