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G8NF95 (G8NF95_BRUSS) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL AEU06275.1
Synonyms:ddl HAMAP-Rule MF_00047
ORF Names:BSVBI22_A1267 EMBL AEU06275.1
OrganismBrucella suis VBI22 [Complete proteome] EMBL AEU06275.1
Taxonomic identifier1112912 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain141 – 346206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3001Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3131Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3131Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G8NF95 [UniParc].

Last modified February 22, 2012. Version 1.
Checksum: 655D7EED0CE1366E

FASTA35337,560
        10         20         30         40         50         60 
MASGKMRIVV LFGGRSAEHD VSVLSATNVM NALDPAKYEA VPVFVTRAGQ WLLSRFVNGA 

        70         80         90        100        110        120 
LEKPSSGAEL CLVPGGCGRA IVVPDAGAPY EADKIDIIFP VLHGLHGEDG AVQGLAQVAR 

       130        140        150        160        170        180 
VPLAGCGIPG SANALDKDIA KRLVNEAGLS TAKSVTITRE EVPAFSALEQ ALGLPIFIKP 

       190        200        210        220        230        240 
ARQGSSVGVH KVVTEADYQA AMSDGFTYDD KLLAEEFIQA REVECGVLED EGGALFVSRA 

       250        260        270        280        290        300 
GEIVPAESHC FYSYDAKYID ADGTEIKVPA ELPEQVENEI RAIATRAFRV LGCDSMARVD 

       310        320        330        340        350 
FFVTADRRIV LNEINTIPGF TDMSMYSKVM AVSGVSYPEI INRLVAHGLA RGS 

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References

[1]"Complete Genome Sequence of Brucella suis VBI22, Isolated from Bovine Milk."
Tae H., Shallom S., Settlage R., Hawkins G.N., Adams L.G., Garner H.R.
J. Bacteriol. 194:910-910(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: VBI22 EMBL AEU06275.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003128 Genomic DNA. Translation: AEU06275.1.
RefSeqYP_005154947.1. NC_016797.1.

3D structure databases

ProteinModelPortalG8NF95.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEU06275; AEU06275; BSVBI22_A1267.
GeneID11728760.
KEGGbsv:BSVBI22_A1267.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycBSUI1112912:GJTI-1290-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG8NF95_BRUSS
AccessionPrimary (citable) accession number: G8NF95
Entry history
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: February 19, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)