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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Geobacillus thermoleovorans CCB_US3_UF5
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311ATP; via amide nitrogenUniRule annotation
Metal bindingi123 – 1231Magnesium; catalyticUniRule annotation
Active sitei147 – 1471Proton acceptorUniRule annotation
Binding sitei174 – 1741Allosteric activator ADPUniRule annotation
Binding sitei182 – 1821Substrate; shared with dimeric partnerUniRule annotation
Binding sitei231 – 2311Allosteric activator ADPUniRule annotation
Binding sitei242 – 2421SubstrateUniRule annotation
Binding sitei263 – 2631Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi92 – 932ATPUniRule annotation
Nucleotide bindingi122 – 1254ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciGTHE1111068:GJY8-3148-MONOMER.
UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
ORF Names:GTCCBUS3UF5_30850Imported
OrganismiGeobacillus thermoleovorans CCB_US3_UF5Imported
Taxonomic identifieri1111068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
ProteomesiUP000005636: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 455Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni145 – 1473Substrate bindingUniRule annotation
Regioni189 – 1913Substrate bindingUniRule annotation
Regioni205 – 2073Allosteric activator ADP bindingUniRule annotation
Regioni233 – 2353Allosteric activator ADP bindingUniRule annotation
Regioni269 – 2724Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G8N4X7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKKTYEVGR VQFDANEVEM MKRIGVLTSG GDSPGMNAAI RAVVRKAIYH
60 70 80 90 100
GVEVYGIYHG YAGLIAGNIK KLEVGDVGDI IHRGGTILYT ARCPEFKTEE
110 120 130 140 150
GQKKGIEQLK KHGIEGLVVI GGDGSYQGAK KLTEHGFPCV GVPGTIDNDI
160 170 180 190 200
PGTDFTIGFD TALNTVIDAI DKIRDTATSH ERTYVVEVMG RHAGDIALWS
210 220 230 240 250
GLAGGAETIL IPEADYDMDD IIARLKRGHE RGKKHSIIIV AEGVGSGVDF
260 270 280 290 300
GRQIQEATGF ETRVTVLGHV QRGGSPTAFD RVLASRLGAR AVELLLEGKG
310 320 330
GRCVGIQNNQ IVDHDIAEAL AKTHTVDQRM YTLSKELSI
Length:339
Mass (Da):36,525
Last modified:February 22, 2012 - v1
Checksum:i3A6C3DE2532BEA98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003125 Genomic DNA. Translation: AEV20387.1.
RefSeqiYP_004983487.1. NC_016593.1.

Genome annotation databases

EnsemblBacteriaiAEV20387; AEV20387; GTCCBUS3UF5_30850.
GeneIDi11627314.
KEGGigte:GTCCBUS3UF5_30850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003125 Genomic DNA. Translation: AEV20387.1.
RefSeqiYP_004983487.1. NC_016593.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEV20387; AEV20387; GTCCBUS3UF5_30850.
GeneIDi11627314.
KEGGigte:GTCCBUS3UF5_30850.

Phylogenomic databases

KOiK00850.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.
BioCyciGTHE1111068:GJY8-3148-MONOMER.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of thermophilic Geobacillus thermoleovorans CCB_US3_UF5."
    Muhd Sakaff M.K.L., Abdul Rahman A.Y., Saito J.A., Hou S., Alam M.
    Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: CCB_US3_UF5Imported.

Entry informationi

Entry nameiG8N4X7_GEOTH
AccessioniPrimary (citable) accession number: G8N4X7
Entry historyi
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: February 4, 2015
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.