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G8N4X7

- G8N4X7_GEOTH

UniProt

G8N4X7 - G8N4X7_GEOTH

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Geobacillus thermoleovorans CCB_US3_UF5
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 18 (01 Oct 2014)
      Sequence version 1 (22 Feb 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei31 – 311ATP; via amide nitrogenUniRule annotation
    Metal bindingi123 – 1231Magnesium; catalyticUniRule annotation
    Active sitei147 – 1471Proton acceptorUniRule annotation
    Binding sitei174 – 1741Allosteric activator ADPUniRule annotation
    Binding sitei182 – 1821Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei231 – 2311Allosteric activator ADPUniRule annotation
    Binding sitei242 – 2421SubstrateUniRule annotation
    Binding sitei263 – 2631Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi92 – 932ATPUniRule annotation
    Nucleotide bindingi122 – 1254ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciGTHE1111068:GJY8-3148-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    ORF Names:GTCCBUS3UF5_30850Imported
    OrganismiGeobacillus thermoleovorans CCB_US3_UF5Imported
    Taxonomic identifieri1111068 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
    ProteomesiUP000005636: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 455Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni145 – 1473Substrate bindingUniRule annotation
    Regioni189 – 1913Substrate bindingUniRule annotation
    Regioni205 – 2073Allosteric activator ADP bindingUniRule annotation
    Regioni233 – 2353Allosteric activator ADP bindingUniRule annotation
    Regioni269 – 2724Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    KOiK00850.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    G8N4X7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKKTYEVGR VQFDANEVEM MKRIGVLTSG GDSPGMNAAI RAVVRKAIYH    50
    GVEVYGIYHG YAGLIAGNIK KLEVGDVGDI IHRGGTILYT ARCPEFKTEE 100
    GQKKGIEQLK KHGIEGLVVI GGDGSYQGAK KLTEHGFPCV GVPGTIDNDI 150
    PGTDFTIGFD TALNTVIDAI DKIRDTATSH ERTYVVEVMG RHAGDIALWS 200
    GLAGGAETIL IPEADYDMDD IIARLKRGHE RGKKHSIIIV AEGVGSGVDF 250
    GRQIQEATGF ETRVTVLGHV QRGGSPTAFD RVLASRLGAR AVELLLEGKG 300
    GRCVGIQNNQ IVDHDIAEAL AKTHTVDQRM YTLSKELSI 339
    Length:339
    Mass (Da):36,525
    Last modified:February 22, 2012 - v1
    Checksum:i3A6C3DE2532BEA98
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003125 Genomic DNA. Translation: AEV20387.1.
    RefSeqiYP_004983487.1. NC_016593.1.

    Genome annotation databases

    EnsemblBacteriaiAEV20387; AEV20387; GTCCBUS3UF5_30850.
    GeneIDi11627314.
    KEGGigte:GTCCBUS3UF5_30850.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003125 Genomic DNA. Translation: AEV20387.1 .
    RefSeqi YP_004983487.1. NC_016593.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEV20387 ; AEV20387 ; GTCCBUS3UF5_30850 .
    GeneIDi 11627314.
    KEGGi gte:GTCCBUS3UF5_30850.

    Phylogenomic databases

    KOi K00850.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci GTHE1111068:GJY8-3148-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of thermophilic Geobacillus thermoleovorans CCB_US3_UF5."
      Muhd Sakaff M.K.L., Abdul Rahman A.Y., Saito J.A., Hou S., Alam M.
      Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: CCB_US3_UF5Imported.

    Entry informationi

    Entry nameiG8N4X7_GEOTH
    AccessioniPrimary (citable) accession number: G8N4X7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: February 22, 2012
    Last sequence update: February 22, 2012
    Last modified: October 1, 2014
    This is version 18 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteomeImported

    External Data

    Dasty 3