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G8N4X7

- G8N4X7_GEOTH

UniProt

G8N4X7 - G8N4X7_GEOTH

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, GTCCBUS3UF5_30850
Organism
Geobacillus thermoleovorans CCB_US3_UF5
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311ATP; via amide nitrogen By similarityUniRule annotation
Metal bindingi123 – 1231Magnesium; catalytic By similarityUniRule annotation
Active sitei147 – 1471Proton acceptor By similarityUniRule annotation
Binding sitei174 – 1741Allosteric activator ADP By similarityUniRule annotation
Binding sitei182 – 1821Substrate; shared with dimeric partner By similarityUniRule annotation
Binding sitei231 – 2311Allosteric activator ADP By similarityUniRule annotation
Binding sitei242 – 2421Substrate By similarityUniRule annotation
Binding sitei263 – 2631Substrate; shared with dimeric partner By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi92 – 932ATP By similarityUniRule annotation
Nucleotide bindingi122 – 1254ATP By similarityUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciGTHE1111068:GJY8-3148-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkAUniRule annotation
ORF Names:GTCCBUS3UF5_30850Imported
OrganismiGeobacillus thermoleovorans CCB_US3_UF5Imported
Taxonomic identifieri1111068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
ProteomesiUP000005636: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotationSAAS annotations

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 455Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni145 – 1473Substrate binding By similarityUniRule annotation
Regioni189 – 1913Substrate binding By similarityUniRule annotation
Regioni205 – 2073Allosteric activator ADP binding By similarityUniRule annotation
Regioni233 – 2353Allosteric activator ADP binding By similarityUniRule annotation
Regioni269 – 2724Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

KOiK00850.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G8N4X7-1 [UniParc]FASTAAdd to Basket

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MFKKTYEVGR VQFDANEVEM MKRIGVLTSG GDSPGMNAAI RAVVRKAIYH    50
GVEVYGIYHG YAGLIAGNIK KLEVGDVGDI IHRGGTILYT ARCPEFKTEE 100
GQKKGIEQLK KHGIEGLVVI GGDGSYQGAK KLTEHGFPCV GVPGTIDNDI 150
PGTDFTIGFD TALNTVIDAI DKIRDTATSH ERTYVVEVMG RHAGDIALWS 200
GLAGGAETIL IPEADYDMDD IIARLKRGHE RGKKHSIIIV AEGVGSGVDF 250
GRQIQEATGF ETRVTVLGHV QRGGSPTAFD RVLASRLGAR AVELLLEGKG 300
GRCVGIQNNQ IVDHDIAEAL AKTHTVDQRM YTLSKELSI 339
Length:339
Mass (Da):36,525
Last modified:February 22, 2012 - v1
Checksum:i3A6C3DE2532BEA98
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003125 Genomic DNA. Translation: AEV20387.1.
RefSeqiYP_004983487.1. NC_016593.1.

Genome annotation databases

EnsemblBacteriaiAEV20387; AEV20387; GTCCBUS3UF5_30850.
GeneIDi11627314.
KEGGigte:GTCCBUS3UF5_30850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003125 Genomic DNA. Translation: AEV20387.1 .
RefSeqi YP_004983487.1. NC_016593.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEV20387 ; AEV20387 ; GTCCBUS3UF5_30850 .
GeneIDi 11627314.
KEGGi gte:GTCCBUS3UF5_30850.

Phylogenomic databases

KOi K00850.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci GTHE1111068:GJY8-3148-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of thermophilic Geobacillus thermoleovorans CCB_US3_UF5."
    Muhd Sakaff M.K.L., Abdul Rahman A.Y., Saito J.A., Hou S., Alam M.
    Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: CCB_US3_UF5Imported.

Entry informationi

Entry nameiG8N4X7_GEOTH
AccessioniPrimary (citable) accession number: G8N4X7
Entry historyi
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: September 3, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome

External Data

Dasty 3

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