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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Geobacillus thermoleovorans CCB_US3_UF5
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31ATP; via amide nitrogenUniRule annotation1
Metal bindingi123Magnesium; catalyticUniRule annotation1
Active sitei147Proton acceptorUniRule annotation1
Binding sitei174Allosteric activator ADPUniRule annotation1
Binding sitei182Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei231Allosteric activator ADPUniRule annotation1
Binding sitei242SubstrateUniRule annotation1
Binding sitei263Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi92 – 93ATPUniRule annotation2
Nucleotide bindingi122 – 125ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
ORF Names:GTCCBUS3UF5_30850Imported
OrganismiGeobacillus thermoleovorans CCB_US3_UF5Imported
Taxonomic identifieri1111068 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
Proteomesi
  • UP000005636 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 295PFKInterPro annotationAdd BLAST273

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 45Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni145 – 147Substrate bindingUniRule annotation3
Regioni189 – 191Substrate bindingUniRule annotation3
Regioni205 – 207Allosteric activator ADP bindingUniRule annotation3
Regioni233 – 235Allosteric activator ADP bindingUniRule annotation3
Regioni269 – 272Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK00850.
OrthoDBiPOG091H01AC.

Family and domain databases

CDDicd00763. Bacterial_PFK. 1 hit.
HAMAPiMF_00339. Phosphofructokinase_I_B1. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G8N4X7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKKTYEVGR VQFDANEVEM MKRIGVLTSG GDSPGMNAAI RAVVRKAIYH
60 70 80 90 100
GVEVYGIYHG YAGLIAGNIK KLEVGDVGDI IHRGGTILYT ARCPEFKTEE
110 120 130 140 150
GQKKGIEQLK KHGIEGLVVI GGDGSYQGAK KLTEHGFPCV GVPGTIDNDI
160 170 180 190 200
PGTDFTIGFD TALNTVIDAI DKIRDTATSH ERTYVVEVMG RHAGDIALWS
210 220 230 240 250
GLAGGAETIL IPEADYDMDD IIARLKRGHE RGKKHSIIIV AEGVGSGVDF
260 270 280 290 300
GRQIQEATGF ETRVTVLGHV QRGGSPTAFD RVLASRLGAR AVELLLEGKG
310 320 330
GRCVGIQNNQ IVDHDIAEAL AKTHTVDQRM YTLSKELSI
Length:339
Mass (Da):36,525
Last modified:February 22, 2012 - v1
Checksum:i3A6C3DE2532BEA98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003125 Genomic DNA. Translation: AEV20387.1.

Genome annotation databases

EnsemblBacteriaiAEV20387; AEV20387; GTCCBUS3UF5_30850.
KEGGigte:GTCCBUS3UF5_30850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003125 Genomic DNA. Translation: AEV20387.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEV20387; AEV20387; GTCCBUS3UF5_30850.
KEGGigte:GTCCBUS3UF5_30850.

Phylogenomic databases

KOiK00850.
OrthoDBiPOG091H01AC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

CDDicd00763. Bacterial_PFK. 1 hit.
HAMAPiMF_00339. Phosphofructokinase_I_B1. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG8N4X7_GEOTH
AccessioniPrimary (citable) accession number: G8N4X7
Entry historyi
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: November 30, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.