ID   G8N3E1_GEOTH            Unreviewed;       422 AA.
AC   G8N3E1;
DT   22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT   22-FEB-2012, sequence version 1.
DT   27-MAR-2024, entry version 62.
DE   SubName: Full=Putative protease {ECO:0000313|EMBL:AEV20175.1};
GN   ORFNames=GTCCBUS3UF5_28720 {ECO:0000313|EMBL:AEV20175.1};
OS   Geobacillus thermoleovorans CCB_US3_UF5.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC   Geobacillus thermoleovorans group.
OX   NCBI_TaxID=1111068 {ECO:0000313|EMBL:AEV20175.1, ECO:0000313|Proteomes:UP000005636};
RN   [1] {ECO:0000313|EMBL:AEV20175.1, ECO:0000313|Proteomes:UP000005636}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCB_US3_UF5 {ECO:0000313|EMBL:AEV20175.1,
RC   ECO:0000313|Proteomes:UP000005636};
RA   Muhd Sakaff M.K.L., Abdul Rahman A.Y., Saito J.A., Hou S., Alam M.;
RT   "Complete genome sequence of thermophilic Geobacillus thermoleovorans
RT   CCB_US3_UF5.";
RL   Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007829|PDB:4HE5, ECO:0007829|PDB:4HE6}
RP   X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) OF 334-422.
RX   PubMed=23519421; DOI=10.1107/S0907444912050135;
RA   Trillo-Muyo S., Jasilionis A., Domagalski M.J., Chruszcz M., Minor W.,
RA   Kuisiene N., Arolas J.L., Sola M., Gomis-Ruth F.X.;
RT   "Ultratight crystal packing of a 10 kDa protein.";
RL   Acta Crystallogr. D 69:464-470(2013).
CC   -!- SIMILARITY: Belongs to the peptidase U32 family.
CC       {ECO:0000256|ARBA:ARBA00038374}.
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DR   EMBL; CP003125; AEV20175.1; -; Genomic_DNA.
DR   RefSeq; WP_011232027.1; NC_016593.1.
DR   PDB; 4HE5; X-ray; 1.15 A; A=336-422.
DR   PDB; 4HE6; X-ray; 1.10 A; A=334-422.
DR   PDBsum; 4HE5; -.
DR   PDBsum; 4HE6; -.
DR   AlphaFoldDB; G8N3E1; -.
DR   SMR; G8N3E1; -.
DR   MEROPS; U32.002; -.
DR   KEGG; gte:GTCCBUS3UF5_28720; -.
DR   PATRIC; fig|1111068.3.peg.2784; -.
DR   HOGENOM; CLU_011540_0_2_9; -.
DR   Proteomes; UP000005636; Chromosome.
DR   GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR   GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.30.10; Translation factors; 1.
DR   InterPro; IPR001539; Peptidase_U32.
DR   InterPro; IPR032525; Peptidase_U32_C.
DR   InterPro; IPR011060; RibuloseP-bd_barrel.
DR   PANTHER; PTHR30217; PEPTIDASE U32 FAMILY; 1.
DR   PANTHER; PTHR30217:SF6; TRNA HYDROXYLATION PROTEIN P; 1.
DR   Pfam; PF01136; Peptidase_U32; 1.
DR   Pfam; PF16325; Peptidase_U32_C; 1.
DR   SUPFAM; SSF51366; Ribulose-phoshate binding barrel; 1.
DR   PROSITE; PS01276; PEPTIDASE_U32; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:4HE5, ECO:0007829|PDB:4HE6};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000313|EMBL:AEV20175.1}.
FT   DOMAIN          338..419
FT                   /note="Peptidase family U32 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF16325"
SQ   SEQUENCE   422 AA;  47952 MW;  C81F333513B987F9 CRC64;
     MLLKNDRISE IIDGKRVIVK KPELLAPAGN LEKLKIAVHY GADAVFIGGQ EYSLRANADN
     FTIEEIREGV EFANRYGAKV YVTANIYAHN ENIPGLDDYL RALEDAGVCG IIVADPLIIE
     TARRVAPKLE VHLSTQQSLT NWKAVQFWKE EGLERVVLAR EVGAEEIRQI KEKVDIEIEA
     FIHGAMCSAY SGRCVLSNHM TARDSNRGGC CQSCRWDYDL YQLSDGREIP LFEKGDAPFA
     MSAKDLNLIR AIPVMIELGV DSLKIEGRMK SIHYVATVVS VYRKVIDAYC ADPDHFTIRE
     EWVRELEKCA NRETAPSFFD GFPDYTNHMY GTHSRKTTHE FAGLVLGYDP ETGIATVQQR
     NHFRPGDEVE FFGPEIENFT QVIEKIWDED GNELDAARHP LQIVKFKVKR PLFPYNMMRK
     EN
//