G8N349 (G8N349_GEOTH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121
Short name=Asp/Glu-ADT subunit B HAMAP-Rule MF_00121
EC=6.3.5.- HAMAP-Rule MF_00121

Gene names

Name:	gatB HAMAP-Rule MF_00121
ORF Names:	GTCCBUS3UF5_3710 EMBL AEV17697.1

Organism

Geobacillus thermoleovorans CCB_US3_UF5 EMBL AEV17697.1

Taxonomic identifier

1111068 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus › Geobacillus thermoleovorans

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. SAAS SAAS003789 HAMAP-Rule MF_00121
Catalytic activity	ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. SAAS SAAS003789 HAMAP-Rule MF_00121 ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity. SAAS SAAS003789 HAMAP-Rule MF_00121
Sequence similarities	Belongs to the gatB/gatE family. GatB subfamily. HAMAP-Rule MF_00121

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00121
Ligand	ATP-binding HAMAP-Rule MF_00121 Nucleotide-binding
Molecular function	Ligase HAMAP-Rule MF_00121 Transferase EMBL AEV17697.1
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	G8N349 [UniParc]. Last modified February 22, 2012. Version 1. Checksum: EA2ECE1ABA4B68E5	FASTA	476	53,547
	10 20 30 40 50 60 MNFETVIGLE VHVELKTKSK IFSSSPNAFG APPNTQTNVI DLGYPGVLPV LNRQAVEFAM 70 80 90 100 110 120 KAAMALNCEI ATETKFDRKN YFYPDNPKAY QISQYDQPLG RNGWIEIEVN GKKKKIGITR 130 140 150 160 170 180 IHLEEDAGKL THTGDGYSLV DFNRQGTPLI EIVSEPDIRS PEEAYAYLEK LKAIIQYTGV 190 200 210 220 230 240 SDCKMEEGSL RCDANISLRP LGSDKFGTKT ELKNLNSFNF VRMGLEYEAK RQEKILLSGG 250 260 270 280 290 300 VIRQETRRFD EATKTTVLMR VKEGSEDYRY FPEPDLVMLY IDDEWKERVR ASIPELPDAR 310 320 330 340 350 360 RKRYIEEWGL PEYDAKVLTL TKEMADFFEA TVANGADPKL ASNWLMVEVS GYLNAEQKEL 370 380 390 400 410 420 HDIALTPESL AGMIKLIQNG TISSKIAKKV FKELVEHGGD PEKIVKEKGL VQISDEGALR 430 440 450 460 470 KIVLEVLDAN PQSVEDYKNG KDRALGFLVG QVMKATKGQA NPPLVNKLLV EEIGKR

References

[1]	"Complete genome sequence of thermophilic Geobacillus thermoleovorans CCB_US3_UF5." Muhd Sakaff M.K.L., Abdul Rahman A.Y., Saito J.A., Hou S., Alam M. Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: CCB_US3_UF5 EMBL AEV17697.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003125 Genomic DNA. Translation: AEV17697.1.
RefSeq	YP_004980797.1. NC_016593.1.
3D structure databases
ProteinModelPortal	G8N349.
SMR	G8N349. Positions 2-412.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEV17697; AEV17697; GTCCBUS3UF5_3710.
GeneID	11627666.
KEGG	gte:GTCCBUS3UF5_3710.
Phylogenomic databases
KO	K02434.
Family and domain databases
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G8N349_GEOTH

Accession

Primary (citable) accession number: G8N349

Entry history

Integrated into UniProtKB/TrEMBL:	February 22, 2012
Last sequence update:	February 22, 2012
Last modified:	May 1, 2013
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)