G8M2Z5 (G8M2Z5_CLOCD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
ATP-grasp enzyme, D-alanine-D-alanine ligase EMBL AEV69304.1

Gene names

Ordered Locus Names:

Clocl_2750 EMBL AEV69304.1

Organism

Clostridium clariflavum (strain DSM 19732 / NBRC 101661 / EBR45) [Complete proteome] [HAMAP]

Taxonomic identifier

720554 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium clariflavum

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. SAAS SAAS005905
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS005905
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS005905
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS005905
Subcellular location	Cytoplasm By similarity SAAS SAAS005905.
Sequence similarities	Contains 1 ATP-grasp domain. SAAS SAAS005905

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation SAAS SAAS005905 Peptidoglycan synthesis SAAS SAAS005905
Cellular component	Cytoplasm SAAS SAAS005905
Ligand	ATP-binding SAAS SAAS005905 Magnesium SAAS SAAS005905 Manganese SAAS SAAS005905 Metal-binding SAAS SAAS005905 Nucleotide-binding
Molecular function	Ligase SAAS SAAS005905 EMBL AEV69304.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	G8M2Z5 [UniParc]. Last modified February 22, 2012. Version 1. Checksum: BA79EF57E4E4DAA7	FASTA	341	39,288
	10 20 30 40 50 60 MKVRTIGIVY ESKEDALKIA SSVVEKEHVS IDVMYHWRER EEIQAVTAAL NKLGFEVRLL 70 80 90 100 110 120 GTPEDIINNI NIIKKQVDFI FNMSSGFRKR FRLSKVPAIC EAAGIPYSGA DPYTMTVCQN 130 140 150 160 170 180 KHLMKSLWDK MNIPTAPWVF VEDISKLKRL ETSHYPLIIK PAFESNSVGI NANAVAYNKD 190 200 210 220 230 240 ELMLKAETIY GTLHQPLIIE KFISGREFKV FVIGNKREKF TGMIEYLKSN GTSLNSEFIY 250 260 270 280 290 300 FNAKLNGDYR EVKRDINLKD YKHIKSQCEA IYDMFIPLDY GSFDIRVDDE GNHYFLEFNS 310 320 330 340 DVSLHPKKAM AKCCELYGID YVSMVKRILK SAAERWDIVL Q

References

[1]

"Complete sequence of Clostridium clariflavum DSM 19732." US DOE Joint Genome Institute Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Kitzmiller T., Lynd L., Izquierdo J., Woyke T. Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 19732 / NBRC 101661 / EBR45.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003065 Genomic DNA. Translation: AEV69304.1.
RefSeq	YP_005047228.1. NC_016627.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEV69304; AEV69304; Clocl_2750.
GeneID	11563166.
KEGG	ccl:Clocl_2750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01921.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G8M2Z5_CLOCD

Accession

Primary (citable) accession number: G8M2Z5

Entry history

Integrated into UniProtKB/TrEMBL:	February 22, 2012
Last sequence update:	February 22, 2012
Last modified:	April 3, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)