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G8LP77 (G8LP77_ENTCL) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL AEW72337.1
Synonyms:ddl HAMAP-Rule MF_00047
ORF Names:EcWSU1_00897 EMBL AEW72337.1
OrganismEnterobacter cloacae EcWSU1 [Complete proteome] EMBL AEW72337.1
Taxonomic identifier1045856 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Protein attributes

Sequence length380 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain159 – 362204ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding189 – 24456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3161Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3291Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3291Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3311Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G8LP77 [UniParc].

Last modified February 22, 2012. Version 1.
Checksum: 268520DEB63CBF49

FASTA38040,886
        10         20         30         40         50         60 
MVCYWFFVRL EESEMAKQRV GIVFGGKSAE HEVSLQSAKN IVDAIDKSRF DVVLLGIDKQ 

        70         80         90        100        110        120 
GQWHVNDASQ YLLNADDPAH IALNPSAISV ATVPGVVQGQ LIDAGNAQSL AQIDVVFPIV 

       130        140        150        160        170        180 
HGTLGEDGSL QGMLRMANLP FVGSDVLGSA ACMDKDVTKR LLRDAGLNIA PFVTLTRANR 

       190        200        210        220        230        240 
DQHSFAQISG QLGLPLFVKP ANQGSSVGVS KVTSEAQFTQ AVSLAFEFDH KVVVEQGIKG 

       250        260        270        280        290        300 
REIECAVLGN DFPQASTCGE VVLNSDFYSY DTKYIDDKGA QVVVPANIDP AINDKIRAIA 

       310        320        330        340        350        360 
INAYQTLGCS GMARVDVFLT PENEVVINEI NTLPGFTNIS MYPKLWQASG LSYSALITRL 

       370        380 
IELALERHAA DSALKSSVNG 

« Hide

References

[1]"Complete genome of the onion pathogen Enterobacter cloacae EcWSU1."
Humann J.L., Wildung M., Cheng C.H., Lee T., Stewart J.E., Drew J.C., Triplett E.W., Main D., Schroeder B.K.
Stand. Genomic Sci. 5:279-286(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: EcWSU1 EMBL AEW72337.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002886 Genomic DNA. Translation: AEW72337.1.
RefSeqYP_004950758.1. NC_016514.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEW72337; AEW72337; EcWSU1_00897.
GeneID11485376.
KEGGeec:EcWSU1_00897.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycECLO1045856:GHCE-926-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG8LP77_ENTCL
AccessionPrimary (citable) accession number: G8LP77
Entry history
Integrated into UniProtKB/TrEMBL: February 22, 2012
Last sequence update: February 22, 2012
Last modified: April 16, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)