ID   G8LL01_9ENTR            Unreviewed;       883 AA.
AC   G8LL01; W0BDQ7;
DT   22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT   22-FEB-2012, sequence version 1.
DT   24-JAN-2024, entry version 65.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595,
GN   ECO:0000313|EMBL:AEW75843.1};
GN   ORFNames=EcWSU1_04416 {ECO:0000313|EMBL:AEW75843.1};
OS   Enterobacter ludwigii.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
OX   NCBI_TaxID=299767 {ECO:0000313|EMBL:AEW75843.1, ECO:0000313|Proteomes:UP000007838};
RN   [1] {ECO:0000313|EMBL:AEW75843.1, ECO:0000313|Proteomes:UP000007838}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EcWSU1 {ECO:0000313|EMBL:AEW75843.1,
RC   ECO:0000313|Proteomes:UP000007838};
RX   PubMed=22675579; DOI=10.4056/sigs.2174950;
RA   Humann J.L., Wildung M., Cheng C.H., Lee T., Stewart J.E., Drew J.C.,
RA   Triplett E.W., Main D., Schroeder B.K.;
RT   "Complete genome of the onion pathogen Enterobacter cloacae EcWSU1.";
RL   Stand. Genomic Sci. 5:279-286(2011).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC       ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946, ECO:0000256|HAMAP-
CC         Rule:MF_00595};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR   EMBL; CP002886; AEW75843.1; -; Genomic_DNA.
DR   RefSeq; WP_014172182.1; NZ_VTUA01000137.1.
DR   AlphaFoldDB; G8LL01; -.
DR   STRING; 299767.GCA_900068845_04561; -.
DR   GeneID; 45816755; -.
DR   KEGG; eec:EcWSU1_04416; -.
DR   KEGG; elg:BH714_14545; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_6; -.
DR   OrthoDB; 9768133at2; -.
DR   Proteomes; UP000007838; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW   Rule:MF_00595};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW   Pyruvate {ECO:0000313|EMBL:AEW75843.1}.
FT   ACT_SITE        138
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
FT   ACT_SITE        546
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   883 AA;  99290 MW;  6E6FDE8F53057BD2 CRC64;
     MNEQYSALRS NVSMLGKVLG DTIKDALGEN ILDRVETIRK LSKSSRAGNE ASRQELLTTL
     QNLSNDELLP VARAFSQFLN LANTAEQYHS ISPNGEAASN PEVIARTLRK LKDQPDLNED
     IIKKAVESLS LELVLTAHPT EITRRTLIHK MVEVNNCLKQ LDNKDIADYE RNQLMRRLRQ
     LIAQSWHTDE IRKHRPSPVD EAKWGFAVVE NSLWEGVPNY LRELNEQLEA NLDYRLPVDF
     VPVRFTSWMG GDRDGNPNVT AEITRHVLLL SRWKATDLFL KDIQVLISEL SMVEATPELR
     ALAGEEGASE PYRFLMKKLR GQLMATQAWL EARLKGQRLP KPEGLLSQNE QLWEPLYACY
     KSLQACGMGI IANGELLDTL RRVKCFGVPL VRIDVRQEST RHTEALGELT RYLGIGDYES
     WSEADKQAFL IRELNSKRPL LPRNWEPSNE TREVLNTCKA IVDAPKGSVA AYVISMAKTP
     SDVLGVHLLL KEAGIDYALP VAPLFETLDD LNNANDVMTQ LLNIDWYRGF IQGKQMVMIG
     YSDSAKDAGV MAASWAQYQA QDALIKTCEK AGIELTLFHG RGGSIGRGGA PAHAALLSQP
     PGSLKGGLRV TEQGEMIRFK YGLPEVTISS LSLYTSAILE ANLLPPPEPK ESWCHIMDEL
     SDISCDLYRG YVRENKDFVP YFRSATPEQE LGKLPLGSRP AKRRPTGGVE SLRAIPWIFA
     WTQNRLMLPA WLGAGAALQK VVEDGKQNEL ETMCRDWPFF STRLGMLEMV FSKADLWLAE
     YYDQRLVKPE LWTLGKELRE LLEDDIKVVL DIANDSHLMA DLPWIAESIQ LRNIYTDPLN
     VLQAELLHRS RLAEEEGKEP DPRVEQALMV TIAGVAAGMR NTG
//