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G8BAW7

- FAS2_CANPC

UniProt

G8BAW7 - FAS2_CANPC

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Protein
Fatty acid synthase subunit alpha
Gene
FAS2, CPAR2_807400
Organism
Candida parapsilosis (strain CDC 317 / ATCC MYA-4646) (Yeast) (Monilia parapsilosis)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3-oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl-carrier-protein] synthase.1 Publication

Catalytic activityi

Acetyl-CoA + n malonyl-CoA + 2n NADPH = long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP+.UniRule annotation
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].UniRule annotation
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1303 – 13031For beta-ketoacyl synthase activity By similarity
Metal bindingi1770 – 17701Magnesium By similarity
Metal bindingi1771 – 17711Magnesium; via carbonyl oxygen By similarity
Metal bindingi1772 – 17721Magnesium By similarity
Binding sitei1796 – 17961Acetyl-CoA By similarity
Binding sitei1806 – 18061Acetyl-CoA By similarity
Metal bindingi1870 – 18701Magnesium By similarity
Metal bindingi1871 – 18711Magnesium; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB-EC
  2. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
  3. fatty-acyl-CoA synthase activity Source: UniProtKB-EC
  4. holo-[acyl-carrier-protein] synthase activity Source: InterPro
  5. magnesium ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. fatty acid biosynthetic process Source: CGD
  2. macromolecule biosynthetic process Source: InterPro
  3. pathogenesis Source: CGD
  4. single-species biofilm formation on inanimate substrate Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Magnesium, Metal-binding, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid synthase subunit alpha (EC:2.3.1.86)
Including the following 3 domains:
Acyl carrier
3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.100)
Alternative name(s):
Beta-ketoacyl reductase
3-oxoacyl-[acyl-carrier-protein] synthase (EC:2.3.1.41)
Alternative name(s):
Beta-ketoacyl synthase
Gene namesi
Name:FAS2
ORF Names:CPAR2_807400
OrganismiCandida parapsilosis (strain CDC 317 / ATCC MYA-4646) (Yeast) (Monilia parapsilosis)
Taxonomic identifieri578454 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
ProteomesiUP000005221: Chromosome 8

Pathology & Biotechi

Disruption phenotypei

Essential for growth in fatty acid-free medium, but dispensable in medium with fatty acids. The growth in the presence of fatty acid depends on the type. Able to grow in a concentration-dependent manner in the presence of myristic acid, palmitic acid, or Tween 80 as the fatty acid sources, but unable to grow in the presence of unsaturated fatty like acids stearic acid or oleic acid. Intracellular survival in the murine macrophage line J774.16 is significantly reduced compared to wild type or heterozygous fungal cells. Required for systemic infection in mice. Impaired in its capacity to form biofilms on polysterene and silicone surfaces.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18841884Fatty acid synthase subunit alphaUniRule annotation
PRO_0000419251Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei182 – 1821O-(pantetheine 4'-phosphoryl)serine By similarity

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Expressioni

Inductioni

Expression significantly elevated by the presence of glucose. Down-regulated by unsaturated fatty acids oleic acid and Tween 80. Up-regulated during in vitro biofilm formation and hypoxic conditions.2 Publications

Interactioni

Subunit structurei

Fatty acid synthase is composed of alpha and beta subunits By similarity.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini142 – 303162Acyl carrier
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni677 – 873197Beta-ketoacyl reductase By similarity
Add
BLAST
Regioni1147 – 1361215Beta-ketoacyl synthase By similarity
Add
BLAST
Regioni1770 – 17723Acetyl-CoA binding By similarity
Regioni1815 – 183117Acetyl-CoA binding By similarity
Add
BLAST
Regioni1839 – 18424Acetyl-CoA binding By similarity
Regioni1869 – 18713Acetyl-CoA binding By similarity

Sequence similaritiesi

Phylogenomic databases

OrthoDBiEOG76QFRJ.

Family and domain databases

Gene3Di3.40.366.10. 1 hit.
3.40.47.10. 3 hits.
3.40.50.720. 1 hit.
3.90.470.20. 1 hit.
HAMAPiMF_00101. AcpS.
InterProiIPR008278. 4-PPantetheinyl_Trfase_SF.
IPR001227. Ac_transferase_dom.
IPR002582. ACPS.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR026025. FAS_alpha_yeast.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016040. NAD(P)-bd_dom.
IPR004568. PPantethiene-prot_Trfase_dom.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF01648. ACPS. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000454. FAS_yeast_alpha. 1 hit.
ProDomiPD004282. PPantethiene-prot_Trfase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52151. SSF52151. 1 hit.
SSF53901. SSF53901. 4 hits.
SSF56214. SSF56214. 1 hit.
TIGRFAMsiTIGR00556. pantethn_trn. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G8BAW7-1 [UniParc]FASTAAdd to Basket

« Hide

MKPEIEQELS HTLLTELLAY QFASPVRWIE TQDVFLKQHN TERVIEIGPS     50
PTLAGMASRT IKAKYQSYDA ALSLQRQVLC YSKDAKEIYY TPDPAEPPAA 100
EEPKAETGKE SAPAASAAAA AATQPAAAVA PPPQSAGPVE SIPDEPVKAS 150
LLIHVLVAQK LKKPLDAVPM SKAIKDLVNG KSTVQNEILG DLGKEFGSTP 200
EKPEETPLEE LAEQFQDTFS GSLGKTSTSL IGRLMSSKMP GGFSITNARK 250
YLESRFGLGP GRQDSVLLTA LCNEPASRLG SEGDAKSFLD TMAQKYASHA 300
GISLSSPSAG GASSGAGAAV VDSAALDALI AENKKLARQQ LETLARYLQV 350
DLTKGEKAFI KEKEATTVLQ QELDLWEAEH GEFYAKGIKP VFSPLKSRTY 400
DSYWNWARQD LLSMWFDILF GKLTSVDRET INQCIQIMNR ANPTLIKFMQ 450
YHVELCPTYR GETYKLGKRL GEQLIENCKQ ILGQSPVYKD VSRITGPKTT 500
VSAKGDIVYE EANKESVRKF EQYVFEMAQG GSMTKMKQSS IQEDLARVYK 550
AISKQASRDS KLELQKVYDQ LLKVVEGSTE IETEQTTQDA LAIPTGSNTP 600
TEEDELSTAS DDDEIASLPD KTSIAQPVSS TIPNKTIPFL HIQSKSESGN 650
WEYDRKLSSI YLDGLESAAI NGLTFKDKYV LVTGAGAGSI GAEILQGLIS 700
GGAKVIVTTS RYSKKVTEYY QNMYARYGAA GSTLIVVPFN QGSKQDVDAL 750
VEYIYNDQKK GGLGWDLDVI IPFAAIPENG NGIDNIDSKS EFAHRIMLTN 800
LLRLLGAVKA RKTTDTRPAQ CILPLSPNHG TFGFDGLYSE SKISLETLFN 850
RWYSEDWGTK LTICGAIIGW TRGTGLMSAN NIIAEGIEKL GVRTFSQKEM 900
AFNILGLLTP EIVNLCQEEP VMADLNGGLQ FIDNLKEFTS KLRNDLTETA 950
DIRRAVSIES AIEQKVVNGD NVDSNYNKVT VRPRANMKFD FPTLKSYDEI 1000
KQIAPDLEGM LDLENVVVVT GFAEVGPWGN ARTRWEMESK GEFSLEGAIE 1050
MAWIMGMIKY HNGNLKGKPY SGWIDAKTQT PVDDKDIKAK YEEEILEHSG 1100
IRLIEPELFN GYDPKKKQMI QEVVIQHDLE PFECSKETAE QYKHEHGDKC 1150
EISEIEESGE YSVRILKGAT LFIPKALRFD RLVAGQIPTG WDARTYGIPE 1200
DTINQVDPIT LYVLVATVEA LLSAGITDPY EFYKYVHVSE VGNCSGSGMG 1250
GVSALRGMFK DRYADKPVQN DILQESFINT MSAWVNMLLL SASGPIKTPV 1300
GACATAVESV DIGIETILSG KAKVVMVGGY DDFQEEGSYE FANMNATSSA 1350
IDEFKHGRTP KEMSRPTTTT RNGFMEAQGS GIQVIMSADL ALKMGVPIHA 1400
VLAMSATATD KIGRSVPAPG KGILTTAREH HGNLKYPSPL LNVKYRKRQL 1450
SKRLDQIKSW ESSELNYLQE EAHLAKEEFG EEFSEAEFLR ERTEEIYRES 1500
KRQVADAKKQ WGNAFYKSDP RIAPLRGALA TFNLTIDDIG VASFHGTSTV 1550
ANDKNESATI DSMMKHLGRS EGNPVFGVFQ KYLTGHPKGA AGAWMLNGAI 1600
QILESGIVPG NRNADNVDKV LEQYEYVLYP SRSIQTDGIK AVSVTSFGFG 1650
QKGAQAVVVH PDYLYAVLDR STYEDYAKRV TARNKKTYRY MHNAITRNTM 1700
FVAKDKAPYS DELTMDVYLD PLARVSKTKN EFVFTKKSVQ SDKSYVSNIA 1750
NSTAKALSSL NKSSKGVGVD VELLSELNID NETFLERNFT PEEIKYCQNS 1800
ANPQASFTGT WSAKEATFKA LGVSSQGGGA SLKEIEIVRD GNGAPQVVLN 1850
DNAKAAAKAA GVKNVNVSIS HDDFQATAVA LSEF 1884
Length:1,884
Mass (Da):206,941
Last modified:January 25, 2012 - v1
Checksum:i33DCED9F65C58282
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HE605205 Genomic DNA. Translation: CCE42191.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HE605205 Genomic DNA. Translation: CCE42191.1 .

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

OrthoDBi EOG76QFRJ.

Family and domain databases

Gene3Di 3.40.366.10. 1 hit.
3.40.47.10. 3 hits.
3.40.50.720. 1 hit.
3.90.470.20. 1 hit.
HAMAPi MF_00101. AcpS.
InterProi IPR008278. 4-PPantetheinyl_Trfase_SF.
IPR001227. Ac_transferase_dom.
IPR002582. ACPS.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR026025. FAS_alpha_yeast.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016040. NAD(P)-bd_dom.
IPR004568. PPantethiene-prot_Trfase_dom.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF01648. ACPS. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000454. FAS_yeast_alpha. 1 hit.
ProDomi PD004282. PPantethiene-prot_Trfase. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF52151. SSF52151. 1 hit.
SSF53901. SSF53901. 4 hits.
SSF56214. SSF56214. 1 hit.
TIGRFAMsi TIGR00556. pantethn_trn. 1 hit.
PROSITEi PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CDC 317 / ATCC MYA-4646.
  2. "Using RNA-seq to determine the transcriptional landscape and the hypoxic response of the pathogenic yeast Candida parapsilosis."
    Guida A., Lindstaedt C., Maguire S.L., Ding C., Higgins D.G., Corton N.J., Berriman M., Butler G.
    BMC Genomics 12:628-628(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: CDC 317 / ATCC MYA-4646.
  3. "Correlation between biofilm formation and the hypoxic response in Candida parapsilosis."
    Rossignol T., Ding C., Guida A., d'Enfert C., Higgins D.G., Butler G.
    Eukaryot. Cell 8:550-559(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HYPOXIA AND BIOFILM FORMATION.
  4. "Fatty acid synthase impacts the pathobiology of Candida parapsilosis in vitro and during mammalian infection."
    Nguyen L.N., Trofa D., Nosanchuk J.D.
    PLoS ONE 4:E8421-E8421(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY CARBON SOURCES, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiFAS2_CANPC
AccessioniPrimary (citable) accession number: G8BAW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: January 25, 2012
Last modified: February 19, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

It can potentially be a fungicidal target. Inhibition of FAS2 by cerulenin impeds the growth of wild-type and heterozygous strains (1 Publication).

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi