G8BAW7 (FAS2_CANPC) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 11.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Fatty acid synthase subunit alpha EC=2.3.1.86 | ||||
| Gene names |
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| Organism | Candida parapsilosis (strain CDC 317 / ATCC MYA-4646) (Yeast) (Monilia parapsilosis) [Complete proteome] | ||||
| Taxonomic identifier | 578454 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida ›  |
Protein attributes
| Sequence length | 1884 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3-oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl-carrier-protein] synthase. Ref.4 |
| Catalytic activity | Acetyl-CoA + n malonyl-CoA + 2n NADH + 2n NADPH = long-chain-acyl-CoA + n CoA + n CO2 + 2n NAD+ + 2n NADP+. Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein]. (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH. |
| Subunit structure | Fatty acid synthase is composed of alpha and beta subunits By similarity. |
| Induction | Expression significantly elevated by the presence of glucose. Down-regulated by unsaturated fatty acids oleic acid and Tween 80. Up-regulated during in vitro biofilm formation and hypoxic conditions. Ref.3 Ref.4 |
| Disruption phenotype | Essential for growth in fatty acid-free medium, but dispensable in medium with fatty acids. The growth in the presence of fatty acid depends on the type. Able to grow in a concentration-dependent manner in the presence of myristic acid, palmitic acid, or Tween 80 as the fatty acid sources, but unable to grow in the presence of unsaturated fatty like acids stearic acid or oleic acid. Intracellular survival in the murine macrophage line J774.16 is significantly reduced compared to wild type or heterozygous fungal cells. Required for systemic infection in mice. Impaired in its capacity to form biofilms on polysterene and silicone surfaces. Ref.4 |
| Miscellaneous | It can potentially be a fungicidal target. Inhibition of FAS2 by cerulenin impeds the growth of wild-type and heterozygous strains (Ref.4). |
| Sequence similarities | Belongs to the fungal fatty acid synthetase subunit alpha family. Contains 1 acyl carrier domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism |
| Ligand | Magnesium Metal-binding NAD NADP Phosphopantetheine |
| Molecular function | Oxidoreductase Transferase |
| Technical term | Complete proteome Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological_process | fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW macromolecule biosynthetic processInferred from electronic annotation. Source: InterPro |
| Molecular_function | 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Inferred from electronic annotation. Source: EC 3-oxoacyl-[acyl-carrier-protein] synthase activityInferred from electronic annotation. Source: EC fatty-acyl-CoA synthase activityInferred from electronic annotation. Source: EC holo-[acyl-carrier-protein] synthase activityInferred from electronic annotation. Source: InterPro magnesium ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1884 | 1884 | Fatty acid synthase subunit alpha | PRO_0000419251 | |||||
Regions | |||||||||
| Domain | 142 – 303 | 162 | Acyl carrier | ||||||
| Region | 677 – 873 | 197 | Beta-ketoacyl reductase By similarity | ||||||
| Region | 1147 – 1361 | 215 | Beta-ketoacyl synthase By similarity | ||||||
| Region | 1770 – 1772 | 3 | Acetyl-CoA binding By similarity | ||||||
| Region | 1815 – 1831 | 17 | Acetyl-CoA binding By similarity | ||||||
| Region | 1839 – 1842 | 4 | Acetyl-CoA binding By similarity | ||||||
| Region | 1869 – 1871 | 3 | Acetyl-CoA binding By similarity | ||||||
Sites | |||||||||
| Active site | 1303 | 1 | For beta-ketoacyl synthase activity By similarity | ||||||
| Metal binding | 1770 | 1 | Magnesium By similarity | ||||||
| Metal binding | 1771 | 1 | Magnesium; via carbonyl oxygen By similarity | ||||||
| Metal binding | 1772 | 1 | Magnesium By similarity | ||||||
| Metal binding | 1870 | 1 | Magnesium By similarity | ||||||
| Metal binding | 1871 | 1 | Magnesium; via carbonyl oxygen By similarity | ||||||
| Binding site | 1796 | 1 | Acetyl-CoA By similarity | ||||||
| Binding site | 1806 | 1 | Acetyl-CoA By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 182 | 1 | O-(pantetheine 4'-phosphoryl)serine By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Evolution of pathogenicity and sexual reproduction in eight Candida genomes." Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S., Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I., Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C., Fitzpatrick D.A., de Groot P.W.J.  Cuomo C.A.Nature 459:657-662(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 317 / ATCC MYA-4646. |
| [2] | "Using RNA-seq to determine the transcriptional landscape and the hypoxic response of the pathogenic yeast Candida parapsilosis." Guida A., Lindstaedt C., Maguire S.L., Ding C., Higgins D.G., Corton N.J., Berriman M., Butler G. BMC Genomics 12:628-628(2011) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: CDC 317 / ATCC MYA-4646. |
| [3] | "Correlation between biofilm formation and the hypoxic response in Candida parapsilosis." Rossignol T., Ding C., Guida A., d'Enfert C., Higgins D.G., Butler G. Eukaryot. Cell 8:550-559(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY HYPOXIA AND BIOFILM FORMATION. |
| [4] | "Fatty acid synthase impacts the pathobiology of Candida parapsilosis in vitro and during mammalian infection." Nguyen L.N., Trofa D., Nosanchuk J.D. PLoS ONE 4:E8421-E8421(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION BY CARBON SOURCES, DISRUPTION PHENOTYPE. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | HE605205 Genomic DNA. Translation: CCE42191.1. |
3D structure databases | |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| Gene3D | 3.40.366.10. 1 hit. 3.40.47.10. 3 hits. 3.40.50.720. 1 hit. 3.90.470.20. 1 hit. |
| InterPro | IPR008278. 4-PPantetheinyl_Trfase_SF. IPR001227. Ac_transferase_dom. IPR002582. ACPS. IPR016035. Acyl_Trfase/lysoPLipase. IPR026025. FAS_alpha_yeast. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016040. NAD(P)-bd_dom. IPR004568. PPantethiene-prot_Trfase_dom. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view] |
| Pfam | PF01648. ACPS. 1 hit. PF00109. ketoacyl-synt. 1 hit. PF02801. Ketoacyl-synt_C. 1 hit. [Graphical view] |
| PIRSF | PIRSF000454. FAS_yeast_alpha. 1 hit. |
| ProDom | PD004282. PPantethiene-prot_Trfase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SUPFAM | SSF56214. 4-PPT_transf. 1 hit. SSF52151. Acyl_Trfase/lysoPlipase. 1 hit. SSF53901. Thiolase-like. 2 hits. |
| TIGRFAMs | TIGR00556. pantethn_trn. 1 hit. |
| PROSITE | PS50075. ACP_DOMAIN. False negative. PS00606. B_KETOACYL_SYNTHASE. 1 hit. PS00012. PHOSPHOPANTETHEINE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | FAS2_CANPC | ||||||||
| Accession | Primary (citable) accession number: G8BAW7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |