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Protein

D-alanine--D-alanine ligase

Gene

ddlB

Organism
Azospirillum brasilense Sp245
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 magnesium or manganese ions per subunit.UniRule annotation
  • Mn2+SAAS annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi259Magnesium or manganese 1UniRule annotation1
Metal bindingi276Magnesium or manganese 1UniRule annotation1
Metal bindingi276Magnesium or manganese 2UniRule annotation1
Metal bindingi278Magnesium or manganese 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processCell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandATP-bindingPROSITE-ProRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotationSAAS annotation (EC:6.3.2.4UniRule annotationSAAS annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlBImported
Synonyms:ddlUniRule annotation
ORF Names:AZOBR_180105Imported
OrganismiAzospirillum brasilense Sp245Imported
Taxonomic identifieri1064539 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeAzospirillum
Proteomesi
  • UP000007319 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi1064539.AZOBR_180105.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 309ATP-graspInterPro annotationAdd BLAST202

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CPF. Bacteria.
COG1181. LUCA.
KOiK01921.
OrthoDBiPOG091H00GT.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom_sf.
PfamiView protein in Pfam
PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFiPIRSF039102. Ddl/VanB. 2 hits.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

G8AFN0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPHKKHVA VLMGGWSAER EVSLVSGAGV AKALEEEGYR VTAVDVQRDL
60 70 80 90 100
EGLLRALTDP RPDAVFNALH GRGGEDGTIQ GVLEFLGIPY THSGVRAAAV
110 120 130 140 150
AMDKAMTKAL LAPVGVRSPK GLVLTKGELT GGAHPMPAPY IVKPVDEGST
160 170 180 190 200
VGVTLVREGQ NSPVGDAWTF GERALVEEFI PGRELTVGVM GGLDGECRPL
210 220 230 240 250
TVTEIHFEAQ VYDYTAKYSA GHAVHTVPAQ IPEQVAEEAK RLAVLAHRTL
260 270 280 290 300
GCRGVSRSDF RWDDRKSGTD GLFFLEINNQ PGMTPLSLVP EQAAAVGLSY
310
GALVAWMVEN AACQLD
Length:316
Mass (Da):33,617
Last modified:January 25, 2012 - v1
Checksum:i751A4BCA63581E14
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE577327 Genomic DNA. Translation: CCC99035.1.
RefSeqiWP_014241225.1. NC_016617.1.

Genome annotation databases

EnsemblBacteriaiCCC99035; CCC99035; AZOBR_180105.
KEGGiabs:AZOBR_180105.
PATRICifig|192.7.peg.1789.

Similar proteinsi

Entry informationi

Entry nameiG8AFN0_AZOBR
AccessioniPrimary (citable) accession number: G8AFN0
Entry historyiIntegrated into UniProtKB/TrEMBL: January 25, 2012
Last sequence update: January 25, 2012
Last modified: November 22, 2017
This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported