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G8AFN0 (G8AFN0_AZOBR) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlB EMBL CCC99035.1
Synonyms:ddl HAMAP-Rule MF_00047
ORF Names:AZOBR_180105 EMBL CCC99035.1
OrganismAzospirillum brasilense Sp245 [Complete proteome] EMBL CCC99035.1
Taxonomic identifier1064539 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeAzospirillum

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain108 – 309202ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding134 – 18653ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2591Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2761Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2761Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2781Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G8AFN0 [UniParc].

Last modified January 25, 2012. Version 1.
Checksum: 751A4BCA63581E14

FASTA31633,617
        10         20         30         40         50         60 
MTTPHKKHVA VLMGGWSAER EVSLVSGAGV AKALEEEGYR VTAVDVQRDL EGLLRALTDP 

        70         80         90        100        110        120 
RPDAVFNALH GRGGEDGTIQ GVLEFLGIPY THSGVRAAAV AMDKAMTKAL LAPVGVRSPK 

       130        140        150        160        170        180 
GLVLTKGELT GGAHPMPAPY IVKPVDEGST VGVTLVREGQ NSPVGDAWTF GERALVEEFI 

       190        200        210        220        230        240 
PGRELTVGVM GGLDGECRPL TVTEIHFEAQ VYDYTAKYSA GHAVHTVPAQ IPEQVAEEAK 

       250        260        270        280        290        300 
RLAVLAHRTL GCRGVSRSDF RWDDRKSGTD GLFFLEINNQ PGMTPLSLVP EQAAAVGLSY 

       310 
GALVAWMVEN AACQLD 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
HE577327 Genomic DNA. Translation: CCC99035.1.
RefSeqYP_005032397.1. NC_016617.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCCC99035; CCC99035; AZOBR_180105.
GeneID13106589.
KEGGabs:AZOBR_180105.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG8AFN0_AZOBR
AccessionPrimary (citable) accession number: G8AFN0
Entry history
Integrated into UniProtKB/TrEMBL: January 25, 2012
Last sequence update: January 25, 2012
Last modified: July 9, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)