G8AEN2 (G8AEN2_AZOBR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Elongation factor Tu HAMAP-Rule MF_00118
Short name=EF-Tu HAMAP-Rule MF_00118

Gene names

Name:	tufB2 EMBL CCC98855.1
Synonyms:	tuf HAMAP-Rule MF_00118, tufB1 EMBL CCC98841.1
ORF Names:	AZOBR_160001 EMBL CCC98841.1, AZOBR_160015 EMBL CCC98855.1

Organism

Azospirillum brasilense Sp245 EMBL CCC98855.1

Taxonomic identifier

1064539 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Azospirillum › Azospirillum brasilense

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118 RuleBase RU000325
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00118
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00118.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. HAMAP-Rule MF_00118 RuleBase RU000325

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm HAMAP-Rule MF_00118
Ligand	GTP-binding HAMAP-Rule MF_00118 RuleBase RU000323 Nucleotide-binding
Molecular function	Elongation factor HAMAP-Rule MF_00118 RuleBase RU000325 EMBL CCC98855.1
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity HAMAP-Rule MF_00118
	Nucleotide binding	81 – 85	5	GTP By similarity HAMAP-Rule MF_00118
	Nucleotide binding	136 – 139	4	GTP By similarity HAMAP-Rule MF_00118

Sequences

	Sequence		Length	Mass (Da)
	G8AEN2 [UniParc]. Last modified January 25, 2012. Version 1. Checksum: CBA50C0F32A15450	FASTA	396	43,176
	10 20 30 40 50 60 MAKAKFERNK PHCNIGTIGH VDHGKTSLTA AITKVLAESG GATFTAYDQI DKAPEEKARG 70 80 90 100 110 120 ITISTAHVEY ETTNRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI 130 140 150 160 170 180 LLARQVGVPA LVVFMNKVDM VDDPELLELV EMEVRELLSS YQFPGDDIPI VKGSALCALE 190 200 210 220 230 240 DRSPEIGRDA ILKLMAEVDQ YIPQPERPKD RPFLMPIEDV FSISGRGTVV TGRVERGIVK 250 260 270 280 290 300 VGEEVEIVGL KTTVKTTVTG VEMFRKLLDS GEAGDNIGAL LRGTKREDVE RGQVLAKPGS 310 320 330 340 350 360 ITPHTTFKAE AYILTKEEGG RHTPFFTNYR PQFYFRTTDV TGMVKLPEGT EMVMPGDNIS 370 380 390 MEVELIAPIA MDEGLRFAIR EGGRTVGAGV VASIIK

References

[1]

"Azospirillum genomes reveal transition of bacteria from aquatic to terrestrial environments." Wisniewski-Dye F., Borziak K., Khalsa-Moyers G., Alexandre G., Sukharnikov L.O., Wuichet K., Hurst G.B., McDonald W.H., Robertson J.S., Barbe V., Calteau A., Rouy Z., Mangenot S., Prigent-Combaret C., Normand P., Boyer M., Siguier P., Dessaux Y. , Elmerich C., Condemine G., Krishnen G., Kennedy I., Paterson A.H., Gonzalez V., Mavingui P., Zhulin I.B. PLoS Genet. 7:e1002430-e1002430(2011) Cited for: NUCLEOTIDE SEQUENCE. Strain: Sp245 EMBL CCC98855.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HE577327 Genomic DNA. Translation: CCC98841.1. HE577327 Genomic DNA. Translation: CCC98855.1.
RefSeq	YP_005032211.1. NC_016617.1. YP_005032225.1. NC_016617.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CCC98841; CCC98841; AZOBR_160001. CCC98855; CCC98855; AZOBR_160015.
GeneID	13104767. 13106845.
KEGG	abs:AZOBR_160001. abs:AZOBR_160015.
Phylogenomic databases
KO	K02358.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G8AEN2_AZOBR

Accession

Primary (citable) accession number: G8AEN2

Entry history

Integrated into UniProtKB/TrEMBL:	January 25, 2012
Last sequence update:	January 25, 2012
Last modified:	April 3, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)