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G7ZP92

- G7ZP92_STAAU

UniProt

G7ZP92 - G7ZP92_STAAU

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Staphylococcus aureus subsp. aureus MSHR1132
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 18 (01 Oct 2014)
      Sequence version 1 (25 Jan 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
    Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
    Active sitei129 – 1291Proton acceptorUniRule annotation
    Binding sitei156 – 1561Allosteric activator ADPUniRule annotation
    Binding sitei164 – 1641Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei213 – 2131Allosteric activator ADPUniRule annotation
    Binding sitei224 – 2241SubstrateUniRule annotation
    Binding sitei245 – 2451Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 732ATPUniRule annotation
    Nucleotide bindingi102 – 1054ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSAUR985002:GLKP-1584-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    ORF Names:SAMSHR1132_15490Imported
    OrganismiStaphylococcus aureus subsp. aureus MSHR1132Imported
    Taxonomic identifieri985002 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000005887: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni127 – 1293Substrate bindingUniRule annotation
    Regioni171 – 1733Substrate bindingUniRule annotation
    Regioni187 – 1893Allosteric activator ADP bindingUniRule annotation
    Regioni215 – 2173Allosteric activator ADP bindingUniRule annotation
    Regioni251 – 2544Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    KOiK00850.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    G7ZP92-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKIAVLTSG GDSPGMNAAV RAVVRTAIYN EIEVYGVYHG YQGLLNDDIH    50
    KLELGSVGDT IQRGGTFLYS ARCPEFKEQE VRKVAIENLR KRGIEGLVVI 100
    GGDGSYRGAQ RISEECKEIQ TIGIPGTIDN DINGTDFTIG FDTALNTIIG 150
    LVDKIRDTAS SHARTFIIEA MGRDCGDLAL WAGLSVGAET IVVPEVKTDI 200
    KEIADKIEQG IKRGKKHSIV LVAEGCMTAQ ECQKELSEYI NVDNRVSVLG 250
    HVQRGGSPTG ADRVLASRLG GFAVDLLMEG ETAKGVGIKN NKIVATSFDE 300
    IFDGKDHKFD YSLYELANKL SI 322
    Length:322
    Mass (Da):34,840
    Last modified:January 25, 2012 - v1
    Checksum:iAA11B2DA8D59793A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR821777 Genomic DNA. Translation: CCE59390.1.
    RefSeqiWP_000717563.1. NC_016941.1.
    YP_005326059.1. NC_016941.1.

    Genome annotation databases

    EnsemblBacteriaiCCE59390; CCE59390; SAMSHR1132_15490.
    GeneIDi11931668.
    KEGGisuh:SAMSHR1132_15490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR821777 Genomic DNA. Translation: CCE59390.1 .
    RefSeqi WP_000717563.1. NC_016941.1.
    YP_005326059.1. NC_016941.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CCE59390 ; CCE59390 ; SAMSHR1132_15490 .
    GeneIDi 11931668.
    KEGGi suh:SAMSHR1132_15490.

    Phylogenomic databases

    KOi K00850.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci SAUR985002:GLKP-1584-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A very early-branching Staphylococcus aureus lineage lacking the carotenoid pigment staphyloxanthin."
      Holt D.C., Holden M.T., Tong S.Y., Castillo-Ramirez S., Clarke L., Quail M.A., Currie B.J., Parkhill J., Bentley S.D., Feil E.J., Giffard P.M.
      Genome Biol. Evol. 3:881-895(2011)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MSHR1132Imported.

    Entry informationi

    Entry nameiG7ZP92_STAAU
    AccessioniPrimary (citable) accession number: G7ZP92
    Entry historyi
    Integrated into UniProtKB/TrEMBL: January 25, 2012
    Last sequence update: January 25, 2012
    Last modified: October 1, 2014
    This is version 18 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteomeImported

    External Data

    Dasty 3