Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA ligase

Gene

lig

Organism
Methanosaeta harundinacea (strain 6Ac)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei248 – 2481ATPUniRule annotation
Active sitei250 – 2501N6-AMP-lysine intermediateUniRule annotation
Binding sitei255 – 2551ATPUniRule annotation
Binding sitei270 – 2701ATPUniRule annotation
Binding sitei300 – 3001ATPUniRule annotation
Binding sitei340 – 3401ATPUniRule annotation
Binding sitei412 – 4121ATPUniRule annotation
Binding sitei418 – 4181ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMHAR1110509:GLGF-1487-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:Mhar_1487Imported
OrganismiMethanosaeta harundinacea (strain 6Ac)Imported
Taxonomic identifieri1110509 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosaetaceaeMethanosaeta
ProteomesiUP000005877 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliG7WP10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

KOiK10747.
OMAiGRPRPFQ.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G7WP10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSFESFAEL CARIEEIGGS IEKADLLASF LRDLEEEELS VVPSFVMGSP
60 70 80 90 100
FPASSDLLLG VGPSTLYDAL SKAFGAAPSE VQELLRRTGD VGAVAAELAK
110 120 130 140 150
KGRSSTLSTF LEGERLTILE VHSRFLLIAR ASGRRSQTAK MKHLRYLFSE
160 170 180 190 200
AGATEARYMA RLALEDMRIG VGEGIVRDAV AKAFGISPEE VERGYNFTSD
210 220 230 240 250
LGLVARQAKE GRLQELTIEI GRPIKMMLAQ LGSGIAGSVA EMGTAAVEVK
260 270 280 290 300
YDGSRVQIHK RGEEVEIFSR RLERVTRSLP EIVSFVKEGV RAEAAILDGE
310 320 330 340 350
AVARGEDGRP LPFQEILKRF RRKYRVEGAA KRIPLELMLF DLIYLNGRVL
360 370 380 390 400
IDEPLRERRR LLESVADEKI LAGQKVTDDP EEVAGIYEEA IAEGHEGVML
410 420 430 440 450
KSPESPYAPG KRGKNWLKIK PVMETLDLVV IGARWGEGRR ATFFGSYRLA
460 470 480 490 500
SPDPETGRLE DVGWVATGIT DEMLAELTEL FRDLVLVAGE GMEVEVKPEV
510 520 530 540 550
VFEVAFEEIQ KSKSYSSGYA LRFPRLVRIR DDKAVEEADT LDRVAGLYAT
560
QRGRNGRSSG
Length:560
Mass (Da):61,779
Last modified:January 25, 2012 - v1
Checksum:iB700E11D4E8A4EB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003117 Genomic DNA. Translation: AET64851.1.
RefSeqiWP_014587035.1. NC_017527.1.
YP_005920474.1. NC_017527.1.

Genome annotation databases

EnsemblBacteriaiAET64851; AET64851; Mhar_1487.
GeneIDi12510656.
KEGGimhi:Mhar_1487.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003117 Genomic DNA. Translation: AET64851.1.
RefSeqiWP_014587035.1. NC_017527.1.
YP_005920474.1. NC_017527.1.

3D structure databases

ProteinModelPortaliG7WP10.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAET64851; AET64851; Mhar_1487.
GeneIDi12510656.
KEGGimhi:Mhar_1487.

Phylogenomic databases

KOiK10747.
OMAiGRPRPFQ.

Enzyme and pathway databases

BioCyciMHAR1110509:GLGF-1487-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome characteristics and predicted function of methyl-group oxidation pathway in the obligate aceticlastic methanogens, Methanosaeta spp."
    Zhu J., Zheng H., Ai G., Zhang G., Liu D., Liu X., Dong X.
    PLoS ONE 7:E36756-E36756(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 6AcImported.

Entry informationi

Entry nameiG7WP10_METH6
AccessioniPrimary (citable) accession number: G7WP10
Entry historyi
Integrated into UniProtKB/TrEMBL: January 25, 2012
Last sequence update: January 25, 2012
Last modified: May 27, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.