Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acyl carrier protein

Gene

acpP

Organism
Escherichia coli (strain 'clone D i14')
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Carrier of the growing fatty acid chain in fatty acid biosynthesis.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371PhosphopantetheineCombined sources

GO - Molecular functioni

  1. ACP phosphopantetheine attachment site binding involved in fatty acid biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Fatty acid biosynthesisUniRule annotationSAAS annotation, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciECOL885275:GJE6-1262-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl carrier proteinUniRule annotation
Short name:
ACPUniRule annotation
Gene namesi
Name:acpPUniRule annotationImported
Ordered Locus Names:i14_1248Imported
OrganismiEscherichia coli (strain 'clone D i14')Imported
Taxonomic identifieri885275 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000005864: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei37 – 371O-(pantetheine 4'-phosphoryl)serineUniRule annotation

Post-translational modificationi

4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.UniRule annotation
4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by acpS.UniRule annotation

Keywords - PTMi

PhosphopantetheineCombined sources, PhosphoproteinUniRule annotationSAAS annotation

Proteomic databases

PRIDEiG7RM21.

Interactioni

Protein-protein interaction databases

DIPiDIP-60690N.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IHFX-ray2.10G/H/I/J/K/L1-78[»]
4IHGX-ray2.89G/H/I/J/K/L1-78[»]
4IHHX-ray2.13G/H/I/J/K/L1-78[»]
ProteinModelPortaliG7RM21.
SMRiG7RM21. Positions 2-78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 acyl carrier domain.UniRule annotation

Phylogenomic databases

KOiK02078.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
HAMAPiMF_01217. Acyl_carrier.
InterProiIPR003231. Acyl_carrier.
IPR009081. Acyl_carrier_prot-like.
IPR006162. Ppantetheine_attach_site.
[Graphical view]
PfamiPF00550. PP-binding. 1 hit.
[Graphical view]
ProDomiPD000887. PD000887. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47336. SSF47336. 1 hit.
TIGRFAMsiTIGR00517. acyl_carrier. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G7RM21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIEERVKK IIGEQLGVKQ EEVTNNASFV EDLGADSLDT VELVMALEEE
60 70
FDTEIPDEEA EKITTVQAAI DYINGHQA
Length:78
Mass (Da):8,640
Last modified:January 25, 2012 - v1
Checksum:i41FCFC39D45BFEA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002212 Genomic DNA. Translation: AER88748.1.
RefSeqiYP_006153372.1. NC_017652.1.

Genome annotation databases

EnsemblBacteriaiAER88748; AER88748; i14_1248.
GeneIDi12663967.
KEGGielc:i14_1248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002212 Genomic DNA. Translation: AER88748.1.
RefSeqiYP_006153372.1. NC_017652.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IHFX-ray2.10G/H/I/J/K/L1-78[»]
4IHGX-ray2.89G/H/I/J/K/L1-78[»]
4IHHX-ray2.13G/H/I/J/K/L1-78[»]
ProteinModelPortaliG7RM21.
SMRiG7RM21. Positions 2-78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60690N.

Proteomic databases

PRIDEiG7RM21.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAER88748; AER88748; i14_1248.
GeneIDi12663967.
KEGGielc:i14_1248.

Phylogenomic databases

KOiK02078.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciECOL885275:GJE6-1262-MONOMER.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
HAMAPiMF_01217. Acyl_carrier.
InterProiIPR003231. Acyl_carrier.
IPR009081. Acyl_carrier_prot-like.
IPR006162. Ppantetheine_attach_site.
[Graphical view]
PfamiPF00550. PP-binding. 1 hit.
[Graphical view]
ProDomiPD000887. PD000887. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47336. SSF47336. 1 hit.
TIGRFAMsiTIGR00517. acyl_carrier. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rates of mutation and host transmission for an Escherichia coli clone over 3 years."
    Reeves P.R., Liu B., Zhou Z., Li D., Guo D., Ren Y., Clabots C., Lan R., Johnson J.R., Wang L.
    PLoS ONE 6:E26907-E26907(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 'clone D i14'Imported.
  2. "Chasing acyl carrier protein through a catalytic cycle of lipid A production."
    Masoudi A., Raetz C.R., Zhou P., Pemble C.W.
    Nature 505:422-426(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH PHOSPHOPANTETHEINE.

Entry informationi

Entry nameiG7RM21_ECOC1
AccessioniPrimary (citable) accession number: G7RM21
Entry historyi
Integrated into UniProtKB/TrEMBL: January 25, 2012
Last sequence update: January 25, 2012
Last modified: March 4, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.