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G7RJW9 (G7RJW9_ECOC1) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 6. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase B HAMAP-Rule MF_01080
EC=5.4.99.25 HAMAP-Rule MF_01080
Alternative name(s):
tRNA pseudouridine(55) synthase HAMAP-Rule MF_01080
tRNA pseudouridylate synthase HAMAP-Rule MF_01080
tRNA-uridine isomerase HAMAP-Rule MF_01080
Gene names
Name:truB HAMAP-Rule MF_01080 EMBL AER91063.1
Ordered Locus Names:i14_3610 EMBL AER91063.1
OrganismEscherichia coli (strain 'clone D i14') [Complete proteome] [HAMAP]
Taxonomic identifier885275 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080 SAAS SAAS014780

Catalytic activity

tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080 SAAS SAAS014780

Sequence similarities

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. HAMAP-Rule MF_01080

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site481Nucleophile By similarity HAMAP-Rule MF_01080
Binding site431Substrate By similarity HAMAP-Rule MF_01080
Binding site761Substrate By similarity HAMAP-Rule MF_01080
Binding site1791Substrate By similarity HAMAP-Rule MF_01080
Binding site2001Substrate By similarity HAMAP-Rule MF_01080

Sequences

Sequence LengthMass (Da)Tools
G7RJW9 [UniParc].

Last modified January 25, 2012. Version 1.
Checksum: FEA46C01E0E8E7A5

FASTA31435,087
        10         20         30         40         50         60 
MSRPRRRGRD INGVLLLDKP QGMSSNDALQ KVKRIYNANR AGHTGALDPL ATGMLPICLG 

        70         80         90        100        110        120 
EATKFSQYLL DSDKRYRVIA RLGQRTDTSD ADGQIVEERP VTFSAEQLAA ALDTFRGDIE 

       130        140        150        160        170        180 
QIPSMYSALK YQGKKLYEYA RQGIEVPREA RPITVYELLF IRHEGNELEL EIHCSKGTYI 

       190        200        210        220        230        240 
RTIIDDLGEK LGCGAHVIYL RRLAVSKYPV ERMVTLEHLR ELVEQAEQQD IPAAELLDPL 

       250        260        270        280        290        300 
LMPMDSPASD YPVVNLPLTS SVYFKNGNPV RTSGAPLEGL VRVTEGENGK FIGMGEIDDE 

       310 
GRVAPRRLVV EYPA

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References

[1]"Rates of mutation and host transmission for an Escherichia coli clone over 3 years."
Reeves P.R., Liu B., Zhou Z., Li D., Guo D., Ren Y., Clabots C., Lan R., Johnson J.R., Wang L.
PLoS ONE 6:E26907-E26907(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 'clone D i14'.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002212 Genomic DNA. Translation: AER91063.1.
RefSeqYP_006155687.1. NC_017652.1.

3D structure databases

ProteinModelPortalG7RJW9.
SMRG7RJW9. Positions 10-311.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAER91063; AER91063; i14_3610.
GeneID12666330.
KEGGelc:i14_3610.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03177.

Family and domain databases

HAMAPMF_01080. TruB_bact.
InterProIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR015947. PUA-like_domain.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
[Graphical view]
PANTHERPTHR13767. PTHR13767. 1 hit.
PfamPF09157. TruB-C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMSSF55120. PsdUridine_synth_cat_dom. 1 hit.
SSF88697. PUA-like. 1 hit.
TIGRFAMsTIGR00431. TruB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameG7RJW9_ECOC1
AccessionPrimary (citable) accession number: G7RJW9
Entry history
Integrated into UniProtKB/TrEMBL: January 25, 2012
Last sequence update: January 25, 2012
Last modified: April 3, 2013
This is version 6 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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