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Protein

ATP-dependent 6-phosphofructokinase

Gene

11438579

Organism
Medicago truncatula (Barrel medic) (Medicago tribuloides)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (11426025), Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase (11434432)
  3. ATP-dependent 6-phosphofructokinase (25490151), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (11445901), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (11439570), ATP-dependent 6-phosphofructokinase (11405809), ATP-dependent 6-phosphofructokinase (11410489), ATP-dependent 6-phosphofructokinase (25494344), ATP-dependent 6-phosphofructokinase (11438579), ATP-dependent 6-phosphofructokinase (11430664), ATP-dependent 6-phosphofructokinase (11423706), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (11434359), ATP-dependent 6-phosphofructokinase (11415742)
  4. Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase (25485453), Fructose-bisphosphate aldolase (11445395), Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase (11440200), Fructose-bisphosphate aldolase (11433706), Fructose-bisphosphate aldolase (25494828), Fructose-bisphosphate aldolase (25483885), Fructose-bisphosphate aldolase, Fructose-bisphosphate aldolase (25492390)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei98ATP; via amide nitrogenUniRule annotation1
Metal bindingi187Magnesium; catalyticUniRule annotation1
Sitei188Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei217Proton acceptorUniRule annotation1
Binding sitei316SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi161 – 162ATPUniRule annotation2
Nucleotide bindingi186 – 189ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzymeUniRule annotation, KinaseUniRule annotationImported, Transferase
Biological processGlycolysisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:11438579Imported
Synonyms:PFKUniRule annotation
Ordered Locus Names:MTR_6g090130Imported
OrganismiMedicago truncatula (Barrel medic) (Medicago tribuloides)Imported
Taxonomic identifieri3880 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago
Proteomesi
  • UP000002051 Componenti: Chromosome 6

Subcellular locationi

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 397PFKInterPro annotationAdd BLAST306

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 217Substrate bindingUniRule annotation3
Regioni260 – 262Substrate bindingUniRule annotation3
Regioni373 – 376Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850
OMAiWRYTNLG
OrthoDBiEOG093609PL

Family and domain databases

HAMAPiMF_01981 Phosphofructokinase_II_X, 1 hit
InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
IPR012004 PyroP-dep_PFK_TP0108
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000534 PPi_PFK_TP0108, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Complete.

G7KHY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLTEPKVVT GPAGYVLEDV PHLSDYIPDL PTYPNPLQDN PAYSVVKQYF
60 70 80 90 100
VHVDDTVPQK VVVHKDSPRG VHFRRAGPRQ KVYFESDEIQ AAIVTCGGLC
110 120 130 140 150
PGLNTVIREL VCSLHHMYGV TRVLGIEGGY RGFYARNTIT LTPSSVNNIH
160 170 180 190 200
KRGGTILGTS RGGHDTNKIV DSIQDRGINQ VYIIGGDGTQ RGASVIFEEV
210 220 230 240 250
RRRGLKCAVV GIPKTIDNDI PIIDKSFGFD TAVEEAQRAI NAAHVEAESF
260 270 280 290 300
ENGIGVVKLM GRYSGFIAMY ATLASRDVDC CLIPESPFYL EGPGGLYEYI
310 320 330 340 350
DRRLKENGHM VIVIAEGAGQ ELLTESLQSG KKQDASGNKL LQDVGLWISQ
360 370 380 390 400
SIKDHFAREK TLPITLKYID PTYMIRAVPS NASDNVYCTL LAQSAVHGAM
410 420 430 440 450
AGYTGYTSGL VNGRQTYIPF YRITERQNNV VITDRMWARL LSSTNQPSFM
460 470 480 490 500
EVKVSDEDTK GENSLDELPD GHCSEDTSVD EITHNLGCLA PLAVPVLRTG

GISGV
Length:505
Mass (Da):55,211
Last modified:January 25, 2012 - v1
Checksum:iA8EDBD79C00E34E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM001222 Genomic DNA Translation: AES76981.1
RefSeqiXP_003620763.1, XM_003620715.2

Genome annotation databases

EnsemblPlantsiAES76981; AES76981; MTR_6g090130
GeneIDi11438579
GrameneiAES76981; AES76981; MTR_6g090130
KEGGimtr:MTR_6g090130

Similar proteinsi

Entry informationi

Entry nameiG7KHY6_MEDTR
AccessioniPrimary (citable) accession number: G7KHY6
Entry historyiIntegrated into UniProtKB/TrEMBL: January 25, 2012
Last sequence update: January 25, 2012
Last modified: March 28, 2018
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health