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Protein

Alpha-amylase

Gene

MTR_1g019440

Organism
Medicago truncatula (Barrel medic) (Medicago tribuloides)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei201NucleophileUniRule annotation1
Active sitei226Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotation, Hydrolase

Keywords - Biological processi

Carbohydrate metabolismUniRule annotation

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Gene namesi
Ordered Locus Names:MTR_1g019440Imported
OrganismiMedicago truncatula (Barrel medic) (Medicago tribuloides)Imported
Taxonomic identifieri3880 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago
Proteomesi
  • UP000002051 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_500766209922 – 421Alpha-amylaseSequence analysisAdd BLAST400

Proteomic databases

PRIDEiG7I3K2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 361AamyInterPro annotationAdd BLAST338
Domaini362 – 421Alpha-amyl_C2InterPro annotationAdd BLAST60

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

KOiK01176.
OMAiDNHDTGP.
OrthoDBiEOG093600FS.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

G7I3K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSLPWLSLF CFCLSIFPLL ASSTLLFQGF NWESSNKGGW YNSLKNLIPD
60 70 80 90 100
LANAGITHVW LPPPSQSVGP QGYLPGRLYD LDASKYGSKD DLKSLIAAFK
110 120 130 140 150
DKGINCLADI VINHRTAERK DDRGIYCLFE GGTPDSKLDW GPSFICKDDT
160 170 180 190 200
AYSDGTGNLD SGEGYQAAPD IDHLNPQVQK ELSEWMNWLK TEIGFSGWRF
210 220 230 240 250
DFVKGYAPSI TKIYMENTSP DFAVGEYWNS LSYGQDGKLN YNQDAARGEL
260 270 280 290 300
VNWVENGGGV VNAFDFTTKG ILQAAVQGEL WRLKDSNGKP PGLIGIKPEN
310 320 330 340 350
GATFIDNHDT GSTQKLWPFP SDKVMQGYAY ILTHPGTPSI FYDHFFDWGL
360 370 380 390 400
KDQIAKLTAI RQRNGINMKS TVNILAADAD LYVAKIDNKI IVKIGPRMDL
410 420
GNLIPSNFHV ATSGQDYAVW E
Length:421
Mass (Da):46,715
Last modified:January 25, 2012 - v1
Checksum:iC62A20D01A979CD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM001217 Genomic DNA. Translation: AES59437.1.
RefSeqiXP_003589186.1. XM_003589138.2.

Genome annotation databases

EnsemblPlantsiAES59437; AES59437; MTR_1g019440.
GeneIDi11419190.
GrameneiAES59437; AES59437; MTR_1g019440.
KEGGimtr:MTR_1g019440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM001217 Genomic DNA. Translation: AES59437.1.
RefSeqiXP_003589186.1. XM_003589138.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEiG7I3K2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAES59437; AES59437; MTR_1g019440.
GeneIDi11419190.
GrameneiAES59437; AES59437; MTR_1g019440.
KEGGimtr:MTR_1g019440.

Phylogenomic databases

KOiK01176.
OMAiDNHDTGP.
OrthoDBiEOG093600FS.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG7I3K2_MEDTR
AccessioniPrimary (citable) accession number: G7I3K2
Entry historyi
Integrated into UniProtKB/TrEMBL: January 25, 2012
Last sequence update: January 25, 2012
Last modified: October 5, 2016
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.