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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Salmonella enterica subsp. enterica serovar Adelaide str. A4-669
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotationSAAS annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotationSAAS annotation
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.UniRule annotationSAAS annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotationSAAS annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotationSAAS annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei161Important for catalytic activityUniRule annotation1
Sitei195Important for catalytic activityUniRule annotation1
Binding sitei352SubstrateUniRule annotation1
Binding sitei380NAD; via amide nitrogenUniRule annotation1
Binding sitei399NADUniRule annotation1
Binding sitei463NADUniRule annotation1
Binding sitei485NADUniRule annotation1
Active sitei506For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation1
Binding sitei509NADUniRule annotation1
Binding sitei556SubstrateUniRule annotation1
Binding sitei716SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi456 – 458NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseUniRule annotationSAAS annotation, LyaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation
Biological processFatty acid metabolismUniRule annotationSAAS annotation, Lipid degradationUniRule annotationSAAS annotation, Lipid metabolism
LigandNADUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
ORF Names:LTSEADE_5477Imported
OrganismiSalmonella enterica subsp. enterica serovar Adelaide str. A4-669Imported
Taxonomic identifieri913063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000004906 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini372 – 5503HCDH_NInterPro annotationAdd BLAST179
Domaini552 – 6483HCDHInterPro annotationAdd BLAST97
Domaini683 – 7453HCDHInterPro annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 245Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST245
Regioni367 – 7853-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST419

Sequence similaritiesi

In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotationSAAS annotation

Family and domain databases

HAMAPiMF_01621. FadB. 1 hit.
InterProiView protein in InterPro
IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH-like_C_sf.
IPR029045. ClpP/crotonase-like_dom_sf.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR001753. Enoyl-CoA_hydra/iso.
IPR012799. FadB.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH_1. 1 hit.
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF51735. SSF51735. 1 hit.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02437. FadB. 1 hit.
PROSITEiView protein in PROSITE
PS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

G5LGR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISAERDHIG ISLIIYLHFI NTKYTLRFIW YDQITLWIQE TDMLYKGDTL
60 70 80 90 100
YLDWLEDGIA ELVFDAPGSV NKLDTATVAS LGQALEVLEK QHDLKGLLLR
110 120 130 140 150
SNKAAFIVGA DITEFLSLFL VPEEQLSQWL HFANSVFNRL EDLPVPTLAA
160 170 180 190 200
VNGYALGGGC ECVLATDYRL ATPDPTPDLR IGLLATPDLR IGLPETKLGI
210 220 230 240 250
MPGFGGSVRL PRMLGADSAL EIIAAGKDVG AEHALKIGLV DGVVKQEKLI
260 270 280 290 300
EGAIAVLRQA ITGDLDWRAK RQPKLEPLKL SKIEAAMSFT IAKGMVAQTA
310 320 330 340 350
GKHYPAPMTA VKTIEAAARF GREEALNLEN KSFVPLAHTN EARALVGIFL
360 370 380 390 400
NDQYVKGKAK KLTKDIETPK QAAVLGAGIM GGGIAYQSAW KGVPVIMKDI
410 420 430 440 450
SDKSLNLGMT EAAKLLNKQL ERGKIDGLKL ASVISTIHPT LDYAGFDRVD
460 470 480 490 500
VVVEAVVENP KVKKAVLAET EQKVRPETVL ASNTSTIPIG ELASALERPE
510 520 530 540 550
NFCGMHFFNP VHRMPLVEII RGEKSSDETI AKVVAWASKM GKTPIVVNDC
560 570 580 590 600
PGFFVNRVLF PYFAGFSQLL RDGADFRKVD KVMEKQFGWP MGPAYLLDVV
610 620 630 640 650
GIDTAHHAQA VMAAGFPQRM QKEYRDAIDA LFDASRFGQK NGLGFWRYKE
660 670 680 690 700
DSKGKPKKEE DAAVDDLLAS VSQPKRDFSD DEIIARMMIP MINEVVRCLE
710 720 730 740 750
EGIIASPAEA DMALVYGLGF PPFHGGAFRW LDTQGSAKYL DMAQQYQHLG
760 770 780
PLYEVPEGLR NKARHNEPYY PPVEPARPVG SLKTA
Length:785
Mass (Da):86,226
Last modified:January 25, 2012 - v1
Checksum:iAE6A184D397FD021
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AFCI01001816 Genomic DNA. Translation: EHC29731.1.

Genome annotation databases

EnsemblBacteriaiEHC29731; EHC29731; LTSEADE_5477.
PATRICifig|913063.3.peg.4285.

Similar proteinsi

Entry informationi

Entry nameiG5LGR1_SALET
AccessioniPrimary (citable) accession number: G5LGR1
Entry historyiIntegrated into UniProtKB/TrEMBL: January 25, 2012
Last sequence update: January 25, 2012
Last modified: February 28, 2018
This is version 43 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported