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G5EFL0

- GLD4_CAEEL

UniProt

G5EFL0 - GLD4_CAEEL

Protein

Poly(A) RNA polymerase gld-4

Gene

gld-4

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 23 (01 Oct 2014)
      Sequence version 1 (14 Dec 2011)
      Previous versions | rss
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    Functioni

    Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. The enzymatic activity is enhanced by its interaction with gls-1. Required, together with gld-2, for early meiotic progression in male and female germ cells and for gld-1 protein accumulation in the hermaphrodite germline. In the germline, forms a complex with gls-1 which directly binds to gld-1 mRNA and prevents its degradation.1 Publication

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi139 – 1391Magnesium or manganese; catalyticBy similarityCurated
    Metal bindingi141 – 1411Magnesium or manganese; catalyticBy similarityCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: WormBase

    GO - Biological processi

    1. meiotic nuclear division Source: UniProtKB-KW
    2. mRNA polyadenylation Source: WormBase
    3. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Transferase

    Keywords - Biological processi

    Meiosis, mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) RNA polymerase gld-41 Publication (EC:2.7.7.191 Publication)
    Alternative name(s):
    Defective in germ line development protein 4
    Germline development defective-41 Publication
    Gene namesi
    Name:gld-4Imported
    ORF Names:ZK858.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome I

    Organism-specific databases

    WormBaseiZK858.1; CE42850; WBGene00014115; gld-4.

    Subcellular locationi

    Cytoplasm 1 Publication. Cytoplasmic granule 1 Publication. Cytoplasmperinuclear region 1 Publication
    Note: Localizes to P granules. This association is less apparent during pachytene, becomes obvious in maturing oocytes and is most prominently visible in developing embryos.1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: WormBase
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    3. P granule Source: WormBase

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    High embryonic lethality. Hermaphrodite animals are partially fertile and those that produce sperm and oocytes have reduced brood sizes and display smaller germlines. Hermaphrodite gld-4 and gld-2 double mutants are fully sterile and display either an earlier meiotic arrest than gld-2 single mutant or a failure to commit to meiotic progression and a return to mitosis.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi139 – 1391D → A: Loss of catalytic activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 845845Poly(A) RNA polymerase gld-4PRO_0000419046Add
    BLAST

    Proteomic databases

    PRIDEiG5EFL0.

    Expressioni

    Tissue specificityi

    Germline-specific.1 Publication

    Developmental stagei

    Expressed both maternally and zygotically throughout embryonic development and in adult hermaphrodites.1 Publication

    Interactioni

    Subunit structurei

    Interacts with gls-1 isoform C.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliG5EFL0.
    SMRiG5EFL0. Positions 83-372.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini276 – 33560PAP-associatedSequence AnalysisAdd
    BLAST

    Domaini

    In contrast to other poly(A) RNA polymerases, lacks any RNA-binding domain.1 Publication

    Sequence similaritiesi

    Contains 1 PAP-associated domain.Sequence Analysis

    Phylogenomic databases

    GeneTreeiENSGT00400000022055.
    KOiK03514.
    OMAiKISMFGS.
    OrthoDBiEOG7X6KZP.

    Family and domain databases

    InterProiIPR002934. Nucleotidyltransferase.
    IPR002058. PAP_assoc.
    [Graphical view]
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF03828. PAP_assoc. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    G5EFL0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNEDSRLSSS QQPSTSTPRS SIPSTMNSDE PNTCRRLSQS QEQPSTSRTC    50
    KSETPEFGYS DSLPFAPWRR KRYGLNIQGL HEEIVDMYHW IKPNEIESRL 100
    RTKVFEKVRD SVLRRWKQKT IKISMFGSLR TNLFLPTSDI DVLVECDDWV 150
    GTPGDWLAET ARGLEADNIA ESVMVYGGAF VPIVKMVDRD TRLSIDISFN 200
    TVQGVRAASY IAKVKEEFPL IEPLVLLLKQ FLHYRNLNQT FTGGLSSYGL 250
    VLLLVNFFQL YALNMRSRTI YDRGVNLGHL LLRFLELYSL EFNFEEMGIS 300
    PGQCCYIPKS ASGARYGHKQ AQPGNLALED PLLTANDVGR STYNFSSIAN 350
    AFGQAFQILL VAVTLRERKG KNHVAMRAYK GSLLHLIMPF TSKELTYRNW 400
    LMSGVLSMPG QEAPASYDLN QLHNTLVSPM VDLSRYAWLR KAPAKAEKRD 450
    SRPLTIVNPA DDRQTLAQQL KKQILEQTEA KKSLEKMPAC DDNKKEEELV 500
    ATRETDVELE AEDTESEGHH NGENDLILTG PPLPTSTQSV NTSATVSTAA 550
    SISEREDTDS PGLSSSMGNQ SSEEDEDNGI NNRNNSAVPV QFKKPFNEVV 600
    AQPARESKRT QTTSEDKMQD QFHFNGYSYP PPSRYAAGTA APSHKHRNAH 650
    PQRQRPSIRN LSQGSDGSDE YNVESWNNNI RQGRRASSNS PSPSRQQTNT 700
    RNCGPTNNIP YDSFRSQNKN STLDGSNNSS EEPITMYADV VKKKSSITTS 750
    TNTSTADVNV TNGNPIPANG IIPQSMAVVN VGRGSYRNAL TTSPMTPPSA 800
    HTSMQKQHHL RKDNECGFDN NSATSSTDLS HHQPQLVPPV NRLQR 845
    Length:845
    Mass (Da):94,310
    Last modified:December 14, 2011 - v1
    Checksum:iC4836B6F8E7CC372
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ610054 mRNA. Translation: ACO95123.1.
    Z79759 Genomic DNA. Translation: CAB02138.3.
    RefSeqiNP_492446.3. NM_060045.4.
    UniGeneiCel.16131.

    Genome annotation databases

    EnsemblMetazoaiZK858.1; ZK858.1; WBGene00014115.
    GeneIDi172735.
    KEGGicel:CELE_ZK858.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ610054 mRNA. Translation: ACO95123.1 .
    Z79759 Genomic DNA. Translation: CAB02138.3 .
    RefSeqi NP_492446.3. NM_060045.4.
    UniGenei Cel.16131.

    3D structure databases

    ProteinModelPortali G5EFL0.
    SMRi G5EFL0. Positions 83-372.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi G5EFL0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai ZK858.1 ; ZK858.1 ; WBGene00014115 .
    GeneIDi 172735.
    KEGGi cel:CELE_ZK858.1.

    Organism-specific databases

    CTDi 172735.
    WormBasei ZK858.1 ; CE42850 ; WBGene00014115 ; gld-4.

    Phylogenomic databases

    GeneTreei ENSGT00400000022055.
    KOi K03514.
    OMAi KISMFGS.
    OrthoDBi EOG7X6KZP.

    Miscellaneous databases

    NextBioi 876795.

    Family and domain databases

    InterProi IPR002934. Nucleotidyltransferase.
    IPR002058. PAP_assoc.
    [Graphical view ]
    Pfami PF01909. NTP_transf_2. 1 hit.
    PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two conserved regulatory cytoplasmic poly(A) polymerases, GLD-4 and GLD-2, regulate meiotic progression in C. elegans."
      Schmid M., Kuchler B., Eckmann C.R.
      Genes Dev. 23:824-836(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH GLS-1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASP-139.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiGLD4_CAEEL
    AccessioniPrimary (citable) accession number: G5EFL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: December 14, 2011
    Last modified: October 1, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Miscellaneous

    The gld-4 locus produces a single transcript that is SL-1 trans-spliced.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3