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G5EFL0

- GLD4_CAEEL

UniProt

G5EFL0 - GLD4_CAEEL

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Protein
Poly(A) RNA polymerase gld-4
Gene
gld-4, ZK858.1
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. The enzymatic activity is enhanced by its interaction with gls-1. Required, together with gld-2, for early meiotic progression in male and female germ cells and for gld-1 protein accumulation in the hermaphrodite germline. In the germline, forms a complex with gls-1 which directly binds to gld-1 mRNA and prevents its degradation.1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Magnesium or manganese By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi139 – 1391Magnesium or manganese; catalytic InferredBy similarity
Metal bindingi141 – 1411Magnesium or manganese; catalytic InferredBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: WormBase
Complete GO annotation...

GO - Biological processi

  1. mRNA polyadenylation Source: WormBase
  2. meiotic nuclear division Source: UniProtKB-KW
  3. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Transferase

Keywords - Biological processi

Meiosis, mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase gld-4 (EC:2.7.7.19)
Alternative name(s):
Defective in germ line development protein 4
Germline development defective-4
Gene namesi
Name:gld-4
ORF Names:ZK858.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome I

Organism-specific databases

WormBaseiZK858.1; CE42850; WBGene00014115; gld-4.

Subcellular locationi

Cytoplasm. Cytoplasmic granule. Cytoplasmperinuclear region
Note: Localizes to P granules. This association is less apparent during pachytene, becomes obvious in maturing oocytes and is most prominently visible in developing embryos.1 Publication

GO - Cellular componenti

  1. P granule Source: WormBase
  2. cytoplasm Source: WormBase
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

High embryonic lethality. Hermaphrodite animals are partially fertile and those that produce sperm and oocytes have reduced brood sizes and display smaller germlines. Hermaphrodite gld-4 and gld-2 double mutants are fully sterile and display either an earlier meiotic arrest than gld-2 single mutant or a failure to commit to meiotic progression and a return to mitosis.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi139 – 1391D → A: Loss of catalytic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 845845Poly(A) RNA polymerase gld-4
PRO_0000419046Add
BLAST

Proteomic databases

PRIDEiG5EFL0.

Expressioni

Tissue specificityi

Germline-specific.1 Publication

Developmental stagei

Expressed both maternally and zygotically throughout embryonic development and in adult hermaphrodites.1 Publication

Interactioni

Subunit structurei

Interacts with gls-1 isoform C.1 Publication

Structurei

3D structure databases

ProteinModelPortaliG5EFL0.
SMRiG5EFL0. Positions 83-372.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini276 – 33560PAP-associated
Add
BLAST

Domaini

In contrast to other poly(A) RNA polymerases, lacks any RNA-binding domain.1 Publication

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00400000022055.
KOiK03514.
OMAiKISMFGS.
OrthoDBiEOG7X6KZP.

Family and domain databases

InterProiIPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G5EFL0-1 [UniParc]FASTAAdd to Basket

« Hide

MNEDSRLSSS QQPSTSTPRS SIPSTMNSDE PNTCRRLSQS QEQPSTSRTC    50
KSETPEFGYS DSLPFAPWRR KRYGLNIQGL HEEIVDMYHW IKPNEIESRL 100
RTKVFEKVRD SVLRRWKQKT IKISMFGSLR TNLFLPTSDI DVLVECDDWV 150
GTPGDWLAET ARGLEADNIA ESVMVYGGAF VPIVKMVDRD TRLSIDISFN 200
TVQGVRAASY IAKVKEEFPL IEPLVLLLKQ FLHYRNLNQT FTGGLSSYGL 250
VLLLVNFFQL YALNMRSRTI YDRGVNLGHL LLRFLELYSL EFNFEEMGIS 300
PGQCCYIPKS ASGARYGHKQ AQPGNLALED PLLTANDVGR STYNFSSIAN 350
AFGQAFQILL VAVTLRERKG KNHVAMRAYK GSLLHLIMPF TSKELTYRNW 400
LMSGVLSMPG QEAPASYDLN QLHNTLVSPM VDLSRYAWLR KAPAKAEKRD 450
SRPLTIVNPA DDRQTLAQQL KKQILEQTEA KKSLEKMPAC DDNKKEEELV 500
ATRETDVELE AEDTESEGHH NGENDLILTG PPLPTSTQSV NTSATVSTAA 550
SISEREDTDS PGLSSSMGNQ SSEEDEDNGI NNRNNSAVPV QFKKPFNEVV 600
AQPARESKRT QTTSEDKMQD QFHFNGYSYP PPSRYAAGTA APSHKHRNAH 650
PQRQRPSIRN LSQGSDGSDE YNVESWNNNI RQGRRASSNS PSPSRQQTNT 700
RNCGPTNNIP YDSFRSQNKN STLDGSNNSS EEPITMYADV VKKKSSITTS 750
TNTSTADVNV TNGNPIPANG IIPQSMAVVN VGRGSYRNAL TTSPMTPPSA 800
HTSMQKQHHL RKDNECGFDN NSATSSTDLS HHQPQLVPPV NRLQR 845
Length:845
Mass (Da):94,310
Last modified:December 14, 2011 - v1
Checksum:iC4836B6F8E7CC372
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FJ610054 mRNA. Translation: ACO95123.1.
Z79759 Genomic DNA. Translation: CAB02138.3.
RefSeqiNP_492446.3. NM_060045.4.
UniGeneiCel.16131.

Genome annotation databases

EnsemblMetazoaiZK858.1; ZK858.1; WBGene00014115.
GeneIDi172735.
KEGGicel:CELE_ZK858.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FJ610054 mRNA. Translation: ACO95123.1 .
Z79759 Genomic DNA. Translation: CAB02138.3 .
RefSeqi NP_492446.3. NM_060045.4.
UniGenei Cel.16131.

3D structure databases

ProteinModelPortali G5EFL0.
SMRi G5EFL0. Positions 83-372.
ModBasei Search...

Proteomic databases

PRIDEi G5EFL0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai ZK858.1 ; ZK858.1 ; WBGene00014115 .
GeneIDi 172735.
KEGGi cel:CELE_ZK858.1.

Organism-specific databases

CTDi 172735.
WormBasei ZK858.1 ; CE42850 ; WBGene00014115 ; gld-4.

Phylogenomic databases

GeneTreei ENSGT00400000022055.
KOi K03514.
OMAi KISMFGS.
OrthoDBi EOG7X6KZP.

Miscellaneous databases

NextBioi 876795.

Family and domain databases

InterProi IPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two conserved regulatory cytoplasmic poly(A) polymerases, GLD-4 and GLD-2, regulate meiotic progression in C. elegans."
    Schmid M., Kuchler B., Eckmann C.R.
    Genes Dev. 23:824-836(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH GLS-1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASP-139.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiGLD4_CAEEL
AccessioniPrimary (citable) accession number: G5EFL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: December 14, 2011
Last modified: June 11, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

The gld-4 locus produces a single transcript that is SL-1 trans-spliced.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi