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G5EFL0

- GLD4_CAEEL

UniProt

G5EFL0 - GLD4_CAEEL

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Protein

Poly(A) RNA polymerase gld-4

Gene

gld-4

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. The enzymatic activity is enhanced by its interaction with gls-1. Required, together with gld-2, for early meiotic progression in male and female germ cells and for gld-1 protein accumulation in the hermaphrodite germline. In the germline, forms a complex with gls-1 which directly binds to gld-1 mRNA and prevents its degradation.1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Magnesium or manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi139 – 1391Magnesium or manganese; catalyticBy similarityCurated
Metal bindingi141 – 1411Magnesium or manganese; catalyticBy similarityCurated

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: WormBase

GO - Biological processi

  1. meiotic nuclear division Source: UniProtKB-KW
  2. mRNA polyadenylation Source: WormBase
  3. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Transferase

Keywords - Biological processi

Meiosis, mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase gld-41 Publication (EC:2.7.7.191 Publication)
Alternative name(s):
Defective in germ line development protein 4
Germline development defective-41 Publication
Gene namesi
Name:gld-4Imported
ORF Names:ZK858.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome I

Organism-specific databases

WormBaseiZK858.1; CE42850; WBGene00014115; gld-4.

Subcellular locationi

Cytoplasm 1 Publication. Cytoplasmic granule 1 Publication. Cytoplasmperinuclear region 1 Publication
Note: Localizes to P granules. This association is less apparent during pachytene, becomes obvious in maturing oocytes and is most prominently visible in developing embryos.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: WormBase
  2. P granule Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

High embryonic lethality. Hermaphrodite animals are partially fertile and those that produce sperm and oocytes have reduced brood sizes and display smaller germlines. Hermaphrodite gld-4 and gld-2 double mutants are fully sterile and display either an earlier meiotic arrest than gld-2 single mutant or a failure to commit to meiotic progression and a return to mitosis.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi139 – 1391D → A: Loss of catalytic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 845845Poly(A) RNA polymerase gld-4PRO_0000419046Add
BLAST

Proteomic databases

PRIDEiG5EFL0.

Expressioni

Tissue specificityi

Germline-specific.1 Publication

Developmental stagei

Expressed both maternally and zygotically throughout embryonic development and in adult hermaphrodites.1 Publication

Interactioni

Subunit structurei

Interacts with gls-1 isoform C.1 Publication

Structurei

3D structure databases

ProteinModelPortaliG5EFL0.
SMRiG5EFL0. Positions 83-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini276 – 33560PAP-associatedSequence AnalysisAdd
BLAST

Domaini

In contrast to other poly(A) RNA polymerases, lacks any RNA-binding domain.1 Publication

Sequence similaritiesi

Contains 1 PAP-associated domain.Sequence Analysis

Phylogenomic databases

GeneTreeiENSGT00400000022055.
InParanoidiG5EFL0.
KOiK03514.
OMAiKISMFGS.
OrthoDBiEOG7X6KZP.

Family and domain databases

InterProiIPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

G5EFL0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNEDSRLSSS QQPSTSTPRS SIPSTMNSDE PNTCRRLSQS QEQPSTSRTC
60 70 80 90 100
KSETPEFGYS DSLPFAPWRR KRYGLNIQGL HEEIVDMYHW IKPNEIESRL
110 120 130 140 150
RTKVFEKVRD SVLRRWKQKT IKISMFGSLR TNLFLPTSDI DVLVECDDWV
160 170 180 190 200
GTPGDWLAET ARGLEADNIA ESVMVYGGAF VPIVKMVDRD TRLSIDISFN
210 220 230 240 250
TVQGVRAASY IAKVKEEFPL IEPLVLLLKQ FLHYRNLNQT FTGGLSSYGL
260 270 280 290 300
VLLLVNFFQL YALNMRSRTI YDRGVNLGHL LLRFLELYSL EFNFEEMGIS
310 320 330 340 350
PGQCCYIPKS ASGARYGHKQ AQPGNLALED PLLTANDVGR STYNFSSIAN
360 370 380 390 400
AFGQAFQILL VAVTLRERKG KNHVAMRAYK GSLLHLIMPF TSKELTYRNW
410 420 430 440 450
LMSGVLSMPG QEAPASYDLN QLHNTLVSPM VDLSRYAWLR KAPAKAEKRD
460 470 480 490 500
SRPLTIVNPA DDRQTLAQQL KKQILEQTEA KKSLEKMPAC DDNKKEEELV
510 520 530 540 550
ATRETDVELE AEDTESEGHH NGENDLILTG PPLPTSTQSV NTSATVSTAA
560 570 580 590 600
SISEREDTDS PGLSSSMGNQ SSEEDEDNGI NNRNNSAVPV QFKKPFNEVV
610 620 630 640 650
AQPARESKRT QTTSEDKMQD QFHFNGYSYP PPSRYAAGTA APSHKHRNAH
660 670 680 690 700
PQRQRPSIRN LSQGSDGSDE YNVESWNNNI RQGRRASSNS PSPSRQQTNT
710 720 730 740 750
RNCGPTNNIP YDSFRSQNKN STLDGSNNSS EEPITMYADV VKKKSSITTS
760 770 780 790 800
TNTSTADVNV TNGNPIPANG IIPQSMAVVN VGRGSYRNAL TTSPMTPPSA
810 820 830 840
HTSMQKQHHL RKDNECGFDN NSATSSTDLS HHQPQLVPPV NRLQR
Length:845
Mass (Da):94,310
Last modified:December 14, 2011 - v1
Checksum:iC4836B6F8E7CC372
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FJ610054 mRNA. Translation: ACO95123.1.
Z79759 Genomic DNA. Translation: CAB02138.3.
RefSeqiNP_492446.3. NM_060045.4.
UniGeneiCel.16131.

Genome annotation databases

EnsemblMetazoaiZK858.1; ZK858.1; WBGene00014115.
GeneIDi172735.
KEGGicel:CELE_ZK858.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FJ610054 mRNA. Translation: ACO95123.1 .
Z79759 Genomic DNA. Translation: CAB02138.3 .
RefSeqi NP_492446.3. NM_060045.4.
UniGenei Cel.16131.

3D structure databases

ProteinModelPortali G5EFL0.
SMRi G5EFL0. Positions 83-372.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi G5EFL0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai ZK858.1 ; ZK858.1 ; WBGene00014115 .
GeneIDi 172735.
KEGGi cel:CELE_ZK858.1.

Organism-specific databases

CTDi 172735.
WormBasei ZK858.1 ; CE42850 ; WBGene00014115 ; gld-4.

Phylogenomic databases

GeneTreei ENSGT00400000022055.
InParanoidi G5EFL0.
KOi K03514.
OMAi KISMFGS.
OrthoDBi EOG7X6KZP.

Miscellaneous databases

NextBioi 876795.

Family and domain databases

InterProi IPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two conserved regulatory cytoplasmic poly(A) polymerases, GLD-4 and GLD-2, regulate meiotic progression in C. elegans."
    Schmid M., Kuchler B., Eckmann C.R.
    Genes Dev. 23:824-836(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH GLS-1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASP-139.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiGLD4_CAEEL
AccessioniPrimary (citable) accession number: G5EFL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: December 14, 2011
Last modified: October 29, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

The gld-4 locus produces a single transcript that is SL-1 trans-spliced.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3