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Protein

Disintegrin and metalloproteinase domain-containing protein 10 homolog

Gene

sup-17

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metalloprotease (By similarity). Acts together with protease adm-4 and in a cell autonomous manner to facilitate lin-12/Notch signaling during developmental cell fate decision, including anchor cell/ventral uterine precursor cell decision and vulva precursor cell specification (PubMed:9428412, PubMed:9409830, PubMed:16197940). By modulating lin-12/Notch signaling, plays a role in germline development (PubMed:16197940). Probably by modulating BMP-like Sma/Mab signaling via the shedding of unc-40 ectodomain, involved in the regulation of body size and mesoderm development (PubMed:28068334). Probably by shedding ephrin efn-4, regulates axon guidance of SDQL neuron during development (PubMed:26903502).By similarity5 Publications

Catalytic activityi

Endopeptidase of broad specificity.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi426Zinc; catalyticPROSITE-ProRule annotation1
Active sitei427PROSITE-ProRule annotation1
Metal bindingi430Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi436Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: WormBase

GO - Biological processi

  • cell fate specification Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • membrane protein ectodomain proteolysis Source: WormBase
  • nematode male tail tip morphogenesis Source: UniProtKB
  • Notch signaling pathway Source: WormBase
  • positive regulation of transforming growth factor beta receptor signaling pathway Source: UniProtKB
  • regulation of axon guidance Source: UniProtKB
  • regulation of mesodermal cell fate specification Source: UniProtKB
  • vulval development Source: WormBase

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-CEL-6798695. Neutrophil degranulation.
R-CEL-8957275. Post-translational protein phosphorylation.

Protein family/group databases

MEROPSiM12.328.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 10 homologCurated (EC:3.4.24.81By similarity)
Short name:
ADAM 10 homologCurated
Gene namesi
Name:sup-17Imported
ORF Names:DY3.7Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiDY3.7; CE15751; WBGene00006324; sup-17.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini229 – 745ExtracellularCuratedAdd BLAST517
Transmembranei746 – 766HelicalSequence analysisAdd BLAST21
Topological domaini767 – 922CytoplasmicCuratedAdd BLAST156

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown in a glp-1 (ar202) constitutively active mutant background restores fertility.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi181R → K in n316; reduced body length. Reduced axon migration of SDQL neuron. No pharyngeal defects. In a sma-9 (cc604) mutant background, partially restores the production of the 2 M lineage-derived coelomocytes. In a lin-12 (n952) mutant background, restores egg-laying functions. In a glp-1 (e2141) mutant background, results in sterility in 18 percent adults. 4 Publications1
Mutagenesisi473V → D in n1258; reduced body length. Vulva precursor cells P(5-7).p fail to acquire a secondary cell fate. Males have severe tail patterning defects including shortened and fused rays, smaller fans and crumpled spicules. 13 percent of animals display a twisted pharynx. In a sma-9 (cc604) mutant background, partially restores the production of the 2 M lineage-derived coelomocytes. In a glp-1 (e2141) mutant background, results in 22 percent embryonic lethality and sterility in 30 percent of surviving adults. In a lin-12 (n137) mutant background, prevents the formation of an ectopic pseudovulva. In a lin-12 (ar170) mutant background, enhances the number of adults with 2 anchor cells. In a adm-4 (ok265) mutant background, causes sterility with abnormal oocytes containing endoreduplicated DNA and impaired spermatheca function, and production of 2 anchor cells. In a tsp-12 (ok239) mutant background, causes lethality at various developmental stages. 5 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
PropeptideiPRO_000044139827 – 228By similarityAdd BLAST202
ChainiPRO_5010117176229 – 922Disintegrin and metalloproteinase domain-containing protein 10 homologSequence analysisAdd BLAST694

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi74N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi185N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi346N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi442 ↔ 471PROSITE-ProRule annotation
Glycosylationi475N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi542 ↔ 577By similarity
Disulfide bondi564 ↔ 572By similarity
Disulfide bondi588 ↔ 607PROSITE-ProRule annotation
Disulfide bondi594 ↔ 626By similarity
Disulfide bondi619 ↔ 631By similarity
Glycosylationi632N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi636 ↔ 659By similarity
Disulfide bondi644 ↔ 665By similarity
Disulfide bondi655 ↔ 707By similarity
Glycosylationi677N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi700 ↔ 713By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiG5EFD9.

Expressioni

Tissue specificityi

Expressed in the germline.1 Publication

Developmental stagei

Expressed in embryos, larvae and in adults. Expressed in the developing vulva at the L4 larval stage and in the hypodermis at the L3 larval stage.1 Publication

Gene expression databases

BgeeiWBGene00006324.

Interactioni

Subunit structurei

May interact with tetraspanin tsp-12; the interaction promotes sup-17 cell membrane localization.1 Publication

Protein-protein interaction databases

IntActiG5EFD9. 1 interactor.
STRINGi6239.DY3.7.

Structurei

3D structure databases

SMRiG5EFD9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini242 – 480Peptidase M12BPROSITE-ProRule annotationAdd BLAST239
Domaini511 – 615DisintegrinPROSITE-ProRule annotationAdd BLAST105

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi810 – 881Pro-richPROSITE-ProRule annotationAdd BLAST72

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3658. Eukaryota.
ENOG410XQWB. LUCA.
GeneTreeiENSGT00670000097974.
KOiK06704.
OMAiEGFIQTH.
OrthoDBiEOG091G01J4.

Family and domain databases

CDDicd04270. ZnMc_TACE_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR034025. ADAM10_ADAM17.
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR024079. MetalloPept_cat_dom_sf.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
PfamiView protein in Pfam
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
SMARTiView protein in SMART
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G5EFD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPIRNRLQ LVVTLIFCLF FENVNGLNNF IDNFETLNYR ATHVANQVTR
60 70 80 90 100
RKRSIDSAAS HYQEPIGFRF NAYNRTFHVQ LHPIDDSLFH EDHMSDVDGG
110 120 130 140 150
YADIKPSHFL YEGYLKDDPN SHVHGSVFDG VFEGHIQTGE GRRYSIDKAA
160 170 180 190 200
KYFERDDRPT QYHSIIYRDD EINHRKWRVK RDAENLSEQM QGCGFSSRVR
210 220 230 240 250
REMTDVQNSG ESTDFFTNYM TMGGRSKRAN TLRDHDGLYF VRTCSLYMQA
260 270 280 290 300
DHKLYEHIRM KEGNNDPIRT REEIVSLFYN HIKAVNEIYE GTNFNGIKGL
310 320 330 340 350
HFVIQRTSIY TPDSCDRGRA KTDSDNPFCE ENVDVSNFLN LNSQRNHSAF
360 370 380 390 400
CLAYALTFRD FVGGTLGLAW VASPQFNTAG GICQVHQRYN EGSRGWVYRS
410 420 430 440 450
LNTGIVTLVN YGNRVPARVS QLTLAHEIGH NFGSPHDFPA ECQPGLPDGN
460 470 480 490 500
FIMFASATSG DKPNNGKFSP CSVKNISAVL AVVLKSMPVD PTRNASPVGI
510 520 530 540 550
GKRNCFQERT SAFCGNQIYE PGEECDCGFS QADCDQMGDK CCVPHEARGN
560 570 580 590 600
GGPGPCKRKP GAQCSPSQGY CCNPDTCSLH GKNEEKICRQ ESECSNLQTC
610 620 630 640 650
DGRNAQCPVS PPKHDGIPCQ DSTKVCSSGQ CNGSVCAMFG LEDCFLTEGK
660 670 680 690 700
ADELCFLACI KDGKCTSSVH LPEFSANRTN FLQNMRKDKP GLILHPGSPC
710 720 730 740 750
NNYKGYCDIF RKCRSVDANG PLARLKNLLF NKRTIETLTQ WAQDNWWVVG
760 770 780 790 800
VGGLVFLVIM ALFVKCCAVH TPSTNPNKPP ALNIYQTLTR PGTLIRQHRQ
810 820 830 840 850
RHRAAAGSVP PGPGAQPRSG AASAPSRTTP SARPSAPPLV APQVAVAVPP
860 870 880 890 900
GVVGPPIPLI ATHPGSSSST PAVIVLEPPP PYTAADPGSA MGGPRRGHRK
910 920
NKRQTSSDAA GSSGNGGKKK GK
Length:922
Mass (Da):101,573
Last modified:December 14, 2011 - v1
Checksum:iBAE4E5E65875CDB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024614 mRNA. Translation: AAB97161.1.
BX284601 Genomic DNA. Translation: CAB09416.1.
PIRiT37256.
RefSeqiNP_492377.1. NM_059976.5.
UniGeneiCel.16995.

Genome annotation databases

EnsemblMetazoaiDY3.7; DY3.7; WBGene00006324.
GeneIDi172689.
KEGGicel:CELE_DY3.7.

Similar proteinsi

Entry informationi

Entry nameiADA10_CAEEL
AccessioniPrimary (citable) accession number: G5EFD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2017
Last sequence update: December 14, 2011
Last modified: November 22, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase