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Protein

Disintegrin and metalloproteinase domain-containing protein unc-71

Gene

unc-71

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the migration of sex myoblasts (progenitors of egg-laying muscles), Q neuroblasts and BDU interneurons during development (PubMed:9073451, PubMed:12783787, PubMed:22293500). Involved in axon branching and guidance of neurons including GABAergic type D motor neurons (PubMed:12783787). Promotes sex myoblast migration and positioning independently of gonad attraction cues (PubMed:9073451, PubMed:12783787). May act downstream of mig-13 in order to promote the guidance, migration and positioning of Q neuroblasts and their descendants along the anteroposterior body axis (PubMed:22293500). Required for coordinated movements (PubMed:12783787).3 Publications

GO - Molecular functioni

  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: WormBase

GO - Biological processi

  • cell-cell adhesion Source: WormBase
  • plasma membrane fusion Source: WormBase
  • proteolysis Source: WormBase

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein unc-71Curated
Alternative name(s):
Uncoordinated protein 71Imported
Gene namesi
Name:unc-71Imported
Synonyms:adm-1Imported
ORF Names:Y37D8A.13Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiY37D8A.13; CE20217; WBGene00006804; unc-71.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 746ExtracellularCuratedAdd BLAST723
Transmembranei747 – 767HelicalSequence analysisAdd BLAST21
Topological domaini768 – 1042CytoplasmicCuratedAdd BLAST275

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Defective sex myoblast migration (PubMed:9073451). Motor neuron axon guidance defects with aberrant axon branching in type D motor neurons (PubMed:12783787).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi149G → R in ju161; axon guidance defects. 1 Publication1
Mutagenesisi461D → N in ay64; sex myoblast migration defects. 1 Publication1
Mutagenesisi471L → P in ay46; sex myoblast migration defects. 1 Publication1
Mutagenesisi477C → Y in ay17; sex myoblast migration defects. 1 Publication1
Mutagenesisi504D → N in ju160; axon guidance defects. 1 Publication1
Mutagenesisi509C → Y in ju159; axon guidance defects. 1 Publication1
Mutagenesisi557A → T in e541; uncoordinated movement. 1 Publication1
Mutagenesisi594G → E in ju157; axon guidance defects. 1 Publication1
Mutagenesisi628S → L in ay47; sex myoblast migration defects. 1 Publication1
Mutagenesisi687C → S in ay44; sex myoblast migration defects. 1 Publication1
Mutagenesisi902P → L in ay48; sex myoblast migration defects. 1 Publication1
Mutagenesisi990R → K in ju255; axon guidance defects. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_500895843024 – 1042Disintegrin and metalloproteinase domain-containing protein unc-71CuratedAdd BLAST1019

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi103N-linked (GlcNAc...) asparagine1
Glycosylationi155N-linked (GlcNAc...) asparagine1
Disulfide bondi338 ↔ 426
Disulfide bondi378 ↔ 410
Disulfide bondi380 ↔ 386
Disulfide bondi496 ↔ 516
Glycosylationi538N-linked (GlcNAc...) asparagine1
Disulfide bondi666 ↔ 681
Disulfide bondi675 ↔ 687
Disulfide bondi689 ↔ 698
Glycosylationi703N-linked (GlcNAc...) asparagine1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiG5EFD5.

Expressioni

Developmental stagei

Expressed throughout development (PubMed:8970152, PubMed:12783787, PubMed:22293500). First expressed in several posterior cells of comma stage embryos (PubMed:12783787). Expressed in the excretory cell and in some head neurons in threefold stage embryos (PubMed:12783787). At the L1 stage of larval development expressed in the syncytial hypodermis (PubMed:8970152). From the L1 stage of larval development to adulthood, expressed in head neurons, the excretory cell, excretory gland cells and in the sphincter muscle (PubMed:12783787, PubMed:22293500). From the L4 stage of larval development to adulthood, expressed in hypodermal cells surrounding the vulva (PubMed:12783787).3 Publications

Gene expression databases

BgeeiWBGene00006804.

Interactioni

Protein-protein interaction databases

STRINGi6239.Y37D8A.13.

Structurei

3D structure databases

SMRiG5EFD5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini227 – 431Peptidase M12BAdd BLAST205
Domaini437 – 524DisintegrinAdd BLAST88
Domaini662 – 699EGF-likeAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi205 – 216His-richAdd BLAST12

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607. Eukaryota.
ENOG410XX2M. LUCA.
GeneTreeiENSGT00760000118888.
OMAiHAHCASH.
OrthoDBiEOG091G01L9.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR006586. ADAM_Cys-rich.
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR013032. EGF-like_CS.
IPR013111. EGF_extracell.
IPR024079. MetalloPept_cat_dom_sf.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF07974. EGF_2. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
SMARTiView protein in SMART
SM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G5EFD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MICASKITML GLLVMCTLGG VLGKVDIRQT TANKAFMETM RADGYEVVHP
60 70 80 90 100
FQIRDKNERI GIDTRNYFLK AQEHYSHVTI VIRSNQLGRL KLVLERNNFI
110 120 130 140 150
FLNQTAFHKL DADGERVIQN RVENCYYQGT VGGEESSFVA LSSCNGLRGV
160 170 180 190 200
ISFANGTTFG IWPLDGGDRN SRRHPHILYK SEWSQEAKCG SSMAHAVGQR
210 220 230 240 250
RMKKHVHKHR SHHHEHNKKR DVSKRTKYVE VALIADYEFM KARGLHDLDA
260 270 280 290 300
ISYMLESLNI ADSMLSRDLN IRLSAVYVEL WTDVQRIDLW EDIERTLSGV
310 320 330 340 350
VDYASGHIYH IQKDASILFT AGSFANQEVS NAAIRSICTA RSAVIVKGVE
360 370 380 390 400
QFATHWNGEL LAQSIGHLLG LEHDTTACSC EPSPECVMRQ QPGRVGGGGG
410 420 430 440 450
SPFSWQFSKC SVARMHGIWQ DGNIQCLLNK PFQVSELREC GNGVVDGSEE
460 470 480 490 500
CDCGSRENCQ DPCCDPLTCT LRPHAQCAAH HKCCHRCELR KAGDTCRSSK
510 520 530 540 550
SPCDVAEQCD GKSGDCPPDG HLIDGTVCGT DGQCWRGNCS DSHQQCQKLW
560 570 580 590 600
GREARVAEPV CFEQNTKGAE YANCGQRQAD GTYHPCQIED TRCGTLHCHS
610 620 630 640 650
GSITPIDSSL KAFTFHFTEN SHQIQCKSIA SAAVGLTSDG TNCASGRVCV
660 670 680 690 700
AGSCVEMSSV SSATACPTNN LALLCSGHGH CTTTARCVCF NGWSGVACDI
710 720 730 740 750
RSNSSTYQGS MGFGEEGSGG SSQKSSERKT IMIPHLNIGT TLETATLFAI
760 770 780 790 800
LLGFGVFLLL CLVCLMLCYR RRSVVEIPKP SDEKDEESPD RQIKFGNMPS
810 820 830 840 850
YREEKRKRKS NKKIYGALNR ITEADERDST SLRSRDSAGG SQQLVDRRNG
860 870 880 890 900
APVVVGGIRD PYAGEHIYAE SSSNHLTRQF RGINSDGSYP LRSFGSWRSS
910 920 930 940 950
APISPASSSG QLTDVSTATT PLRLNKIGKF LKTLQSDDES PSPFSDHQSF
960 970 980 990 1000
TTGIGIGARL EQMQFGGGGD EELSAVEADH DVGSNTESSR GCEEPMDPGS
1010 1020 1030 1040
WDSPTLVNGA SSSSTSNNYN FRQSPSLFSD PFKLEMTNSM HN
Length:1,042
Mass (Da):114,196
Last modified:December 14, 2011 - v1
Checksum:iBA6A33593927D4C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68185 mRNA. Translation: AAC47444.1.
BX284603 Genomic DNA. Translation: CAA21545.1.
PIRiT26644.
RefSeqiNP_499680.1. NM_067279.6.
UniGeneiCel.7111.

Genome annotation databases

EnsemblMetazoaiY37D8A.13; Y37D8A.13; WBGene00006804.
GeneIDi176706.
KEGGicel:CELE_Y37D8A.13.

Similar proteinsi

Entry informationi

Entry nameiUNC71_CAEEL
AccessioniPrimary (citable) accession number: G5EFD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2017
Last sequence update: December 14, 2011
Last modified: November 22, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Caution

Contains a metallopeptidase domain, but the active site is not conserved, so the protein is not expected to have protease activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase