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UniProtKB - G5ECR8 (GLCM3_CAEEL)

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Protein

Putative glucosylceramidase 3

Gene

gba-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei259Proton donorBy similarity1
Active sitei364NucleophileBy similarity1

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionHydrolase
Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00222.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glucosylceramidase 3 (EC:3.2.1.45)
Gene namesi
Name:gba-3
ORF Names:F11E6.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF11E6.1a; CE20669; WBGene00008706; gba-3.
F11E6.1b; CE26698; WBGene00008706; gba-3.
F11E6.1c; CE31166; WBGene00008706; gba-3.
F11E6.1d; CE46394; WBGene00008706; gba-3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000042145522 – 522Putative glucosylceramidase 3Add BLAST501

Proteomic databases

PaxDbiG5ECR8.
PeptideAtlasiG5ECR8.

Expressioni

Gene expression databases

BgeeiWBGene00008706.

Interactioni

Protein-protein interaction databases

BioGridi43610. 1 interactor.
IntActiG5ECR8. 1 interactor.
STRINGi6239.F11E6.1d.

Structurei

3D structure databases

ProteinModelPortaliG5ECR8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2566. Eukaryota.
COG5520. LUCA.
GeneTreeiENSGT00390000009464.
HOGENOMiHOG000270031.
InParanoidiG5ECR8.
KOiK01201.
OMAiPIMGDWF.
OrthoDBiEOG091G06I4.
PhylomeDBiG5ECR8.

Family and domain databases

InterProiView protein in InterPro
IPR033452. GH30_C.
IPR001139. Glyco_hydro_30.
IPR033453. Glyco_hydro_30_TIM-barrel.
IPR017853. Glycoside_hydrolase_SF.
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiView protein in Pfam
PF02055. Glyco_hydro_30. 1 hit.
PF17189. Glyco_hydro_30C. 1 hit.
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: G5ECR8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE 
        60         70         80         90        100
PIGEIAEGKA IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER 
       110        120        130        140        150
FQNIFGFGGA FTDSAGDQFV SLSETLQNYI VDSYFGKNGL EYNIGRVPIA 
       160        170        180        190        200
SCDFSTHEYS YDDVHDDFEL KHFALPDEDL KLKIPFIKKA IEKTEGNIQL 
       210        220        230        240        250
FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF FEAYSSHAIT 
       260        270        280        290        300
FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET 
       310        320        330        340        350
LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI 
       360        370        380        390        400
TQRHHPTKFI FGTEACAGYF GHHGPIMGDW FRAESYADDI ITDLNHHVTG 
       410        420        430        440        450
WTDWNLCLDE TGGPNWAYNV VDSPIIVNRT AQEFYKQPMF YALGHFSKFL 
       460        470        480        490        500
PRGSTRVFTK IEGNLAVSAT SVVIEGGRRA TVILSKASNS LLTRIVDSST 
       510        520 
GFSIVLNLPP RSIHTVIWKK RK
Length:522
Mass (Da):59,088
Last modified:December 14, 2011 - v1
Checksum:i3CD73EE50CFBA7C7
GO
Isoform b (identifier: G5ECR8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: FG → AI
     376-376: I → L
     382-382: R → T
     385-385: S → N
     388-388: D → S
     392-392: T → S
     398-398: V → F
     410-411: ET → DM
     415-420: NWAYNV → TWVDNF
     431-431: A → G
     443-443: L → M

Show »
Length:522
Mass (Da):59,081
Checksum:iC36A8CF1E84FBA87
GO
Isoform c (identifier: G5ECR8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: FG → AI
     376-376: I → L
     382-382: R → T
     385-385: S → N
     388-388: D → S
     392-392: T → S
     398-398: V → F
     410-411: ET → DM
     415-420: NWAYNV → TWVDNF
     431-431: A → G
     443-443: L → M
     447-458: SKFLPRGSTRVF → RHVLATLDIMVQ
     459-522: Missing.

Show »
Length:458
Mass (Da):52,212
Checksum:iA177489059901221
GO
Isoform d (identifier: G5ECR8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MSRWKVVILCLLSFMFEI → MAFFSVRSSKIVFGLERSEEK

Show »
Length:525
Mass (Da):59,320
Checksum:i869C84AC63CD3A2A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0457251 – 18MSRWK…FMFEI → MAFFSVRSSKIVFGLERSEE K in isoform d. CuratedAdd BLAST18
Alternative sequenceiVSP_045726370 – 371FG → AI in isoform b and isoform c. Curated2
Alternative sequenceiVSP_045727376I → L in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045728382R → T in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045729385S → N in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045730388D → S in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045731392T → S in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045732398V → F in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045733410 – 411ET → DM in isoform b and isoform c. Curated2
Alternative sequenceiVSP_045734415 – 420NWAYNV → TWVDNF in isoform b and isoform c. Curated6
Alternative sequenceiVSP_045735431A → G in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045736443L → M in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045737447 – 458SKFLP…STRVF → RHVLATLDIMVQ in isoform c. CuratedAdd BLAST12
Alternative sequenceiVSP_045738459 – 522Missing in isoform c. CuratedAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81058, AL031254 Genomic DNA. Translation: CCD31056.1.
Z81058, AL031254 Genomic DNA. Translation: CAB02924.1.
Z81058, AL031254 Genomic DNA. Translation: CAC35813.1.
Z81058 Genomic DNA. Translation: CAD42642.1.
PIRiT18583.
RefSeqiNP_001255965.1. NM_001269036.1. [G5ECR8-4]
NP_503118.1. NM_070717.3. [G5ECR8-2]
NP_503119.1. NM_070718.3. [G5ECR8-1]
UniGeneiCel.13150.

Genome annotation databases

EnsemblMetazoaiF11E6.1a; F11E6.1a; WBGene00008706. [G5ECR8-1]
GeneIDi178535.
KEGGicel:CELE_F11E6.1.
UCSCiF11E6.1a. c. elegans.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLCM3_CAEEL
AccessioniPrimary (citable) accession number: G5ECR8
Secondary accession number(s): G5EEA6, G5EFK0, Q8MQ76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: December 14, 2011
Last modified: June 7, 2017
This is version 44 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families