gba-3

Functionⁱ

Catalytic activityⁱ

D-glucosyl-N-acylsphingosine + H₂O = D-glucose + N-acylsphingosine.

Pathwayⁱ: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Active siteⁱ	259 – 259	1	Proton donorBy similarity
Active siteⁱ	364 – 364	1	NucleophileBy similarity

Enzyme and pathway databases

UniPathwayⁱ	UPA00222.

UniPathwayⁱ

UPA00222.

Protein family/group databases

CAZyⁱ	GH30. Glycoside Hydrolase Family 30.

CAZyⁱ

GH30. Glycoside Hydrolase Family 30.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Putative glucosylceramidase 3 (EC:3.2.1.45)
Gene namesⁱ	Name:gba-3 ORF Names:F11E6.1
Organismⁱ	Caenorhabditis elegans
Taxonomic identifierⁱ	6239 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis
Proteomesⁱ	UP000001940 Componentⁱ: Chromosome IV

Organism-specific databases

WormBaseⁱ	F11E6.1a; CE20669; WBGene00008706; gba-3. F11E6.1b; CE26698; WBGene00008706; gba-3. F11E6.1c; CE31166; WBGene00008706; gba-3. F11E6.1d; CE46394; WBGene00008706; gba-3.

WormBaseⁱ

F11E6.1a; CE20669; WBGene00008706; gba-3.
F11E6.1b; CE26698; WBGene00008706; gba-3.
F11E6.1c; CE31166; WBGene00008706; gba-3.
F11E6.1d; CE46394; WBGene00008706; gba-3.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Signal peptideⁱ	1 – 21	21	Sequence analysis			Add BLAST
Chainⁱ	22 – 522	501	Putative glucosylceramidase 3		PRO_0000421455	Add BLAST

Proteomic databases

PaxDbⁱ	G5ECR8.
PRIDEⁱ	G5ECR8.

Expressionⁱ

Gene expression databases

Bgeeⁱ	WBGene00008706.

Bgeeⁱ

WBGene00008706.

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	43610. 1 interaction.
IntActⁱ	G5ECR8. 1 interaction.
STRINGⁱ	6239.F11E6.1d.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	G5ECR8.
SMRⁱ	G5ECR8. Positions 25-521.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	KOG2566. Eukaryota. COG5520. LUCA.
GeneTreeⁱ	ENSGT00390000009464.
HOGENOMⁱ	HOG000270031.
InParanoidⁱ	G5ECR8.
KOⁱ	K01201.
OMAⁱ	WNLCLDE.
OrthoDBⁱ	EOG091G06I4.
PhylomeDBⁱ	G5ECR8.

Family and domain databases

Gene3Dⁱ	3.20.20.80. 1 hit.
InterProⁱ	IPR033452. GH30_C. IPR001139. Glyco_hydro_30. IPR033453. Glyco_hydro_30_TIM-barrel. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHERⁱ	PTHR11069. PTHR11069. 1 hit.
Pfamⁱ	PF02055. Glyco_hydro_30. 1 hit. PF17189. Glyco_hydro_30C. 1 hit. [Graphical view]
PRINTSⁱ	PR00843. GLHYDRLASE30.
SUPFAMⁱ	SSF51445. SSF51445. 1 hit.

Sequences (4)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform a (identifier: G5ECR8-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE 
        60         70         80         90        100
PIGEIAEGKA IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER 
       110        120        130        140        150
FQNIFGFGGA FTDSAGDQFV SLSETLQNYI VDSYFGKNGL EYNIGRVPIA 
       160        170        180        190        200
SCDFSTHEYS YDDVHDDFEL KHFALPDEDL KLKIPFIKKA IEKTEGNIQL 
       210        220        230        240        250
FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF FEAYSSHAIT 
       260        270        280        290        300
FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET 
       310        320        330        340        350
LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI 
       360        370        380        390        400
TQRHHPTKFI FGTEACAGYF GHHGPIMGDW FRAESYADDI ITDLNHHVTG 
       410        420        430        440        450
WTDWNLCLDE TGGPNWAYNV VDSPIIVNRT AQEFYKQPMF YALGHFSKFL 
       460        470        480        490        500
PRGSTRVFTK IEGNLAVSAT SVVIEGGRRA TVILSKASNS LLTRIVDSST 
       510        520 
GFSIVLNLPP RSIHTVIWKK RK

Length:522

Mass (Da):59,088

Last modified:December 14, 2011 - v1

Checksum:ⁱ3CD73EE50CFBA7C7

GO

Isoform b (identifier: G5ECR8-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: FG → AI
     376-376: I → L
     382-382: R → T
     385-385: S → N
     388-388: D → S
     392-392: T → S
     398-398: V → F
     410-411: ET → DM
     415-420: NWAYNV → TWVDNF
     431-431: A → G
     443-443: L → M

« Hide

        10         20         30         40         50
MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE 
        60         70         80         90        100
PIGEIAEGKA IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER 
       110        120        130        140        150
FQNIFGFGGA FTDSAGDQFV SLSETLQNYI VDSYFGKNGL EYNIGRVPIA 
       160        170        180        190        200
SCDFSTHEYS YDDVHDDFEL KHFALPDEDL KLKIPFIKKA IEKTEGNIQL 
       210        220        230        240        250
FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF FEAYSSHAIT 
       260        270        280        290        300
FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET 
       310        320        330        340        350
LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI 
       360        370        380        390        400
TQRHHPTKFI FGTEACAGYA IHHGPLMGDW FTAENYASDI ISDLNHHFTG 
       410        420        430        440        450
WTDWNLCLDD MGGPTWVDNF VDSPIIVNRT GQEFYKQPMF YAMGHFSKFL 
       460        470        480        490        500
PRGSTRVFTK IEGNLAVSAT SVVIEGGRRA TVILSKASNS LLTRIVDSST 
       510        520 
GFSIVLNLPP RSIHTVIWKK RK

Show »

Length:522

Mass (Da):59,081

Checksum:ⁱC36A8CF1E84FBA87

GO

Isoform c (identifier: G5ECR8-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: FG → AI
     376-376: I → L
     382-382: R → T
     385-385: S → N
     388-388: D → S
     392-392: T → S
     398-398: V → F
     410-411: ET → DM
     415-420: NWAYNV → TWVDNF
     431-431: A → G
     443-443: L → M
     447-458: SKFLPRGSTRVF → RHVLATLDIMVQ
     459-522: Missing.

« Hide

        10         20         30         40         50
MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE 
        60         70         80         90        100
PIGEIAEGKA IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER 
       110        120        130        140        150
FQNIFGFGGA FTDSAGDQFV SLSETLQNYI VDSYFGKNGL EYNIGRVPIA 
       160        170        180        190        200
SCDFSTHEYS YDDVHDDFEL KHFALPDEDL KLKIPFIKKA IEKTEGNIQL 
       210        220        230        240        250
FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF FEAYSSHAIT 
       260        270        280        290        300
FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET 
       310        320        330        340        350
LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI 
       360        370        380        390        400
TQRHHPTKFI FGTEACAGYA IHHGPLMGDW FTAENYASDI ISDLNHHFTG 
       410        420        430        440        450
WTDWNLCLDD MGGPTWVDNF VDSPIIVNRT GQEFYKQPMF YAMGHFRHVL 

ATLDIMVQ

Show »

Length:458

Mass (Da):52,212

Checksum:ⁱA177489059901221

GO

Isoform d (identifier: G5ECR8-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-18: MSRWKVVILCLLSFMFEI → MAFFSVRSSKIVFGLERSEEK

« Hide

        10         20         30         40         50
MAFFSVRSSK IVFGLERSEE KGHASFQCNP KTYDGAFLNI VCVCNATFCD 
        60         70         80         90        100
EIEPIGEIAE GKAIVYRSSL DGDRLKRMSM KMKEKLRKNE SVNVTITIDA 
       110        120        130        140        150
SERFQNIFGF GGAFTDSAGD QFVSLSETLQ NYIVDSYFGK NGLEYNIGRV 
       160        170        180        190        200
PIASCDFSTH EYSYDDVHDD FELKHFALPD EDLKLKIPFI KKAIEKTEGN 
       210        220        230        240        250
IQLFASPWSA PGWMKVTGRM RGGGAMRNDK RVYQAYADYF FKFFEAYSSH 
       260        270        280        290        300
AITFWGLTIQ NEPSTGADMA WRWQTMNYTA ETMRDFLKKY LGPKLKENKL 
       310        320        330        340        350
TETLKVMVLD DGRGLLPGWA DTIFNDPEAT KYADGVAVHW YGNLYSPAVL 
       360        370        380        390        400
LDITQRHHPT KFIFGTEACA GYFGHHGPIM GDWFRAESYA DDIITDLNHH 
       410        420        430        440        450
VTGWTDWNLC LDETGGPNWA YNVVDSPIIV NRTAQEFYKQ PMFYALGHFS 
       460        470        480        490        500
KFLPRGSTRV FTKIEGNLAV SATSVVIEGG RRATVILSKA SNSLLTRIVD 
       510        520 
SSTGFSIVLN LPPRSIHTVI WKKRK

Show »

Length:525

Mass (Da):59,320

Checksum:ⁱ869C84AC63CD3A2A

GO

Alternative sequence

Feature key	Position(s)	Length	Description	Feature identifier	Actions
Alternative sequenceⁱ	1 – 18	18	MSRWK…FMFEI → MAFFSVRSSKIVFGLERSEE K in isoform d. Curated	VSP_045725	Add BLAST
Alternative sequenceⁱ	370 – 371	2	FG → AI in isoform b and isoform c. Curated	VSP_045726
Alternative sequenceⁱ	376 – 376	1	I → L in isoform b and isoform c. Curated	VSP_045727
Alternative sequenceⁱ	382 – 382	1	R → T in isoform b and isoform c. Curated	VSP_045728
Alternative sequenceⁱ	385 – 385	1	S → N in isoform b and isoform c. Curated	VSP_045729
Alternative sequenceⁱ	388 – 388	1	D → S in isoform b and isoform c. Curated	VSP_045730
Alternative sequenceⁱ	392 – 392	1	T → S in isoform b and isoform c. Curated	VSP_045731
Alternative sequenceⁱ	398 – 398	1	V → F in isoform b and isoform c. Curated	VSP_045732
Alternative sequenceⁱ	410 – 411	2	ET → DM in isoform b and isoform c. Curated	VSP_045733
Alternative sequenceⁱ	415 – 420	6	NWAYNV → TWVDNF in isoform b and isoform c. Curated	VSP_045734
Alternative sequenceⁱ	431 – 431	1	A → G in isoform b and isoform c. Curated	VSP_045735
Alternative sequenceⁱ	443 – 443	1	L → M in isoform b and isoform c. Curated	VSP_045736
Alternative sequenceⁱ	447 – 458	12	SKFLP…STRVF → RHVLATLDIMVQ in isoform c. Curated	VSP_045737	Add BLAST
Alternative sequenceⁱ	459 – 522	64	Missing in isoform c. Curated	VSP_045738	Add BLAST

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z81058, AL031254 Genomic DNA. Translation: CCD31056.1. Z81058, AL031254 Genomic DNA. Translation: CAB02924.1. Z81058, AL031254 Genomic DNA. Translation: CAC35813.1. Z81058 Genomic DNA. Translation: CAD42642.1.
PIRⁱ	T18583.
RefSeqⁱ	NP_001255965.1. NM_001269036.1. [G5ECR8-4] NP_503118.1. NM_070717.3. [G5ECR8-2] NP_503119.1. NM_070718.3. [G5ECR8-1]
UniGeneⁱ	Cel.13150.

Genome annotation databases

EnsemblMetazoaⁱ	F11E6.1a; F11E6.1a; WBGene00008706. [G5ECR8-1]
GeneIDⁱ	178535.
KEGGⁱ	cel:CELE_F11E6.1.

Keywords - Coding sequence diversityⁱ

Alternative splicing

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z81058, AL031254 Genomic DNA. Translation: CCD31056.1. Z81058, AL031254 Genomic DNA. Translation: CAB02924.1. Z81058, AL031254 Genomic DNA. Translation: CAC35813.1. Z81058 Genomic DNA. Translation: CAD42642.1.
PIRⁱ	T18583.
RefSeqⁱ	NP_001255965.1. NM_001269036.1. [G5ECR8-4] NP_503118.1. NM_070717.3. [G5ECR8-2] NP_503119.1. NM_070718.3. [G5ECR8-1]
UniGeneⁱ	Cel.13150.

3D structure databases

ProteinModelPortalⁱ	G5ECR8.
SMRⁱ	G5ECR8. Positions 25-521.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	43610. 1 interaction.
IntActⁱ	G5ECR8. 1 interaction.
STRINGⁱ	6239.F11E6.1d.

Protein family/group databases

CAZyⁱ	GH30. Glycoside Hydrolase Family 30.

CAZyⁱ

GH30. Glycoside Hydrolase Family 30.

Proteomic databases

PaxDbⁱ	G5ECR8.
PRIDEⁱ	G5ECR8.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblMetazoaⁱ	F11E6.1a; F11E6.1a; WBGene00008706. [G5ECR8-1]
GeneIDⁱ	178535.
KEGGⁱ	cel:CELE_F11E6.1.

Organism-specific databases

CTDⁱ	178535.
WormBaseⁱ	F11E6.1a; CE20669; WBGene00008706; gba-3. F11E6.1b; CE26698; WBGene00008706; gba-3. F11E6.1c; CE31166; WBGene00008706; gba-3. F11E6.1d; CE46394; WBGene00008706; gba-3.

Phylogenomic databases

eggNOGⁱ	KOG2566. Eukaryota. COG5520. LUCA.
GeneTreeⁱ	ENSGT00390000009464.
HOGENOMⁱ	HOG000270031.
InParanoidⁱ	G5ECR8.
KOⁱ	K01201.
OMAⁱ	WNLCLDE.
OrthoDBⁱ	EOG091G06I4.
PhylomeDBⁱ	G5ECR8.

Enzyme and pathway databases

UniPathwayⁱ	UPA00222.

UniPathwayⁱ

UPA00222.

Miscellaneous databases

PROⁱ	G5ECR8.

PROⁱ

G5ECR8.

Gene expression databases

Bgeeⁱ	WBGene00008706.

Bgeeⁱ

WBGene00008706.

Family and domain databases

Gene3Dⁱ	3.20.20.80. 1 hit.
InterProⁱ	IPR033452. GH30_C. IPR001139. Glyco_hydro_30. IPR033453. Glyco_hydro_30_TIM-barrel. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHERⁱ	PTHR11069. PTHR11069. 1 hit.
Pfamⁱ	PF02055. Glyco_hydro_30. 1 hit. PF17189. Glyco_hydro_30C. 1 hit. [Graphical view]
PRINTSⁱ	PR00843. GLHYDRLASE30.
SUPFAMⁱ	SSF51445. SSF51445. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ

GLCM3_CAEEL

Accessionⁱ

Primary (citable) accession number: G5ECR8
Secondary accession number(s): G5EEA6, G5EFK0, Q8MQ76

Entry historyⁱ

Integrated into UniProtKB/Swiss-Prot:	March 6, 2013
Last sequence update:	December 14, 2011
Last modified:	September 7, 2016
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry statusⁱ

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase
Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries
PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - G5ECR8 (GLCM3_CAEEL)

UniProt

G5ECR8 - GLCM3_CAEEL

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Putative glucosylceramidase 3

Select a section on the left to see content.

<p>Provides any useful information about the protein, mostly biological knowledge.</p><p><a href='../manual/function_section' target='_top'>More...</a></p>Functioni

<p>Describes the metabolic pathway(s) associated with a protein.</p><p><a href='../manual/pathway' target='_top'>More...</a></p>Pathwayi: sphingolipid metabolism

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>Provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.</p><p><a href='../manual/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>Describes post-translational modifications (PTMs) and/or processing events.</p><p><a href='../manual/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>Provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.</p><p><a href='../manual/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>Provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.</p><p><a href='../manual/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>Provides information on the tertiary and secondary structure of a protein.</p><p><a href='../manual/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>Provides information on sequence similarities with other proteins and the domain(s) present in a protein.</p><p><a href='../manual/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>Provides information about the sequence similarity with other proteins.</p><p><a href='../manual/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>Displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.</p><p><a href='../manual/sequences_section' target='_top'>More...</a></p>Sequences (4)i

Alternative sequence

Sequence databases

Genome annotation databases

<p>Is used to point to information related to entries and found in data collections other than UniProtKB.</p><p><a href='../manual/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>Contains any relevant information that doesn’t fit in any other defined sections</p><p><a href='../manual/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: sphingolipid metabolism

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequences (4)ⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ