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G5ECR8 (GLCM3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative glucosylceramidase 3

EC=3.2.1.45
Gene names
Name:gba-3
ORF Names:F11E6.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length522 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Pathway

Lipid metabolism; sphingolipid metabolism.

Sequence similarities

Belongs to the glycosyl hydrolase 30 family.

Ontologies

Keywords
   Biological processLipid metabolism
Sphingolipid metabolism
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

sphingolipid metabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionglucosylceramidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: G5ECR8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: G5ECR8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: FG → AI
     376-376: I → L
     382-382: R → T
     385-385: S → N
     388-388: D → S
     392-392: T → S
     398-398: V → F
     410-411: ET → DM
     415-420: NWAYNV → TWVDNF
     431-431: A → G
     443-443: L → M
Isoform c (identifier: G5ECR8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: FG → AI
     376-376: I → L
     382-382: R → T
     385-385: S → N
     388-388: D → S
     392-392: T → S
     398-398: V → F
     410-411: ET → DM
     415-420: NWAYNV → TWVDNF
     431-431: A → G
     443-443: L → M
     447-458: SKFLPRGSTRVF → RHVLATLDIMVQ
     459-522: Missing.
Isoform d (identifier: G5ECR8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MSRWKVVILCLLSFMFEI → MAFFSVRSSKIVFGLERSEEK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 522501Putative glucosylceramidase 3
PRO_0000421455

Sites

Active site2591Proton donor By similarity
Active site3641Nucleophile By similarity

Natural variations

Alternative sequence1 – 1818MSRWK…FMFEI → MAFFSVRSSKIVFGLERSEE K in isoform d.
VSP_045725
Alternative sequence370 – 3712FG → AI in isoform b and isoform c.
VSP_045726
Alternative sequence3761I → L in isoform b and isoform c.
VSP_045727
Alternative sequence3821R → T in isoform b and isoform c.
VSP_045728
Alternative sequence3851S → N in isoform b and isoform c.
VSP_045729
Alternative sequence3881D → S in isoform b and isoform c.
VSP_045730
Alternative sequence3921T → S in isoform b and isoform c.
VSP_045731
Alternative sequence3981V → F in isoform b and isoform c.
VSP_045732
Alternative sequence410 – 4112ET → DM in isoform b and isoform c.
VSP_045733
Alternative sequence415 – 4206NWAYNV → TWVDNF in isoform b and isoform c.
VSP_045734
Alternative sequence4311A → G in isoform b and isoform c.
VSP_045735
Alternative sequence4431L → M in isoform b and isoform c.
VSP_045736
Alternative sequence447 – 45812SKFLP…STRVF → RHVLATLDIMVQ in isoform c.
VSP_045737
Alternative sequence459 – 52264Missing in isoform c.
VSP_045738

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified December 14, 2011. Version 1.
Checksum: 3CD73EE50CFBA7C7

FASTA52259,088
        10         20         30         40         50         60 
MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE PIGEIAEGKA 

        70         80         90        100        110        120 
IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER FQNIFGFGGA FTDSAGDQFV 

       130        140        150        160        170        180 
SLSETLQNYI VDSYFGKNGL EYNIGRVPIA SCDFSTHEYS YDDVHDDFEL KHFALPDEDL 

       190        200        210        220        230        240 
KLKIPFIKKA IEKTEGNIQL FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF 

       250        260        270        280        290        300 
FEAYSSHAIT FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET 

       310        320        330        340        350        360 
LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI TQRHHPTKFI 

       370        380        390        400        410        420 
FGTEACAGYF GHHGPIMGDW FRAESYADDI ITDLNHHVTG WTDWNLCLDE TGGPNWAYNV 

       430        440        450        460        470        480 
VDSPIIVNRT AQEFYKQPMF YALGHFSKFL PRGSTRVFTK IEGNLAVSAT SVVIEGGRRA 

       490        500        510        520 
TVILSKASNS LLTRIVDSST GFSIVLNLPP RSIHTVIWKK RK 

« Hide

Isoform b [UniParc].

Checksum: C36A8CF1E84FBA87
Show »

FASTA52259,081
Isoform c [UniParc].

Checksum: A177489059901221
Show »

FASTA45852,212
Isoform d [UniParc].

Checksum: 869C84AC63CD3A2A
Show »

FASTA52559,320

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z81058, AL031254 Genomic DNA. Translation: CCD31056.1.
Z81058, AL031254 Genomic DNA. Translation: CAB02924.1.
Z81058, AL031254 Genomic DNA. Translation: CAC35813.1.
Z81058 Genomic DNA. Translation: CAD42642.1.
PIRT18583.
RefSeqNP_001255965.1. NM_001269036.1. [G5ECR8-4]
NP_503118.1. NM_070717.3. [G5ECR8-2]
NP_503119.1. NM_070718.3. [G5ECR8-1]
NP_741501.1. NM_171429.3. [G5ECR8-3]
UniGeneCel.13150.

3D structure databases

ProteinModelPortalG5ECR8.
SMRG5ECR8. Positions 25-521.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActG5ECR8. 1 interaction.

Proteomic databases

PRIDEG5ECR8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF11E6.1a; F11E6.1a; WBGene00008706. [G5ECR8-1]
GeneID178535.
KEGGcel:CELE_F11E6.1.

Organism-specific databases

CTD178535.
WormBaseF11E6.1a; CE20669; WBGene00008706; gba-3.
F11E6.1b; CE26698; WBGene00008706; gba-3.
F11E6.1c; CE31166; WBGene00008706; gba-3.
F11E6.1d; CE46394; WBGene00008706; gba-3.

Phylogenomic databases

eggNOGCOG5520.
GeneTreeENSGT00390000009464.
OMAWNLCLDE.
OrthoDBEOG76DTRZ.
PhylomeDBG5ECR8.

Enzyme and pathway databases

UniPathwayUPA00222.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR11069. PTHR11069. 1 hit.
PfamPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSPR00843. GLHYDRLASE30.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

NextBio901518.

Entry information

Entry nameGLCM3_CAEEL
AccessionPrimary (citable) accession number: G5ECR8
Secondary accession number(s): G5EEA6, G5EFK0, Q8MQ76
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: December 14, 2011
Last modified: June 11, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase