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G5ECR8

- GLCM3_CAEEL

UniProt

G5ECR8 - GLCM3_CAEEL

Protein

Putative glucosylceramidase 3

Gene

gba-3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (14 Dec 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei259 – 2591Proton donorBy similarity
    Active sitei364 – 3641NucleophileBy similarity

    GO - Molecular functioni

    1. glucosylceramidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. sphingolipid metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative glucosylceramidase 3 (EC:3.2.1.45)
    Gene namesi
    Name:gba-3
    ORF Names:F11E6.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome IV

    Organism-specific databases

    WormBaseiF11E6.1a; CE20669; WBGene00008706; gba-3.
    F11E6.1b; CE26698; WBGene00008706; gba-3.
    F11E6.1c; CE31166; WBGene00008706; gba-3.
    F11E6.1d; CE46394; WBGene00008706; gba-3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 522501Putative glucosylceramidase 3PRO_0000421455Add
    BLAST

    Proteomic databases

    PRIDEiG5ECR8.

    Interactioni

    Protein-protein interaction databases

    IntActiG5ECR8. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliG5ECR8.
    SMRiG5ECR8. Positions 25-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 30 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5520.
    GeneTreeiENSGT00390000009464.
    OMAiWNLCLDE.
    OrthoDBiEOG76DTRZ.
    PhylomeDBiG5ECR8.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR11069. PTHR11069. 1 hit.
    PfamiPF02055. Glyco_hydro_30. 1 hit.
    [Graphical view]
    PRINTSiPR00843. GLHYDRLASE30.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: G5ECR8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE    50
    PIGEIAEGKA IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER 100
    FQNIFGFGGA FTDSAGDQFV SLSETLQNYI VDSYFGKNGL EYNIGRVPIA 150
    SCDFSTHEYS YDDVHDDFEL KHFALPDEDL KLKIPFIKKA IEKTEGNIQL 200
    FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF FEAYSSHAIT 250
    FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET 300
    LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI 350
    TQRHHPTKFI FGTEACAGYF GHHGPIMGDW FRAESYADDI ITDLNHHVTG 400
    WTDWNLCLDE TGGPNWAYNV VDSPIIVNRT AQEFYKQPMF YALGHFSKFL 450
    PRGSTRVFTK IEGNLAVSAT SVVIEGGRRA TVILSKASNS LLTRIVDSST 500
    GFSIVLNLPP RSIHTVIWKK RK 522
    Length:522
    Mass (Da):59,088
    Last modified:December 14, 2011 - v1
    Checksum:i3CD73EE50CFBA7C7
    GO
    Isoform b (identifier: G5ECR8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         370-371: FG → AI
         376-376: I → L
         382-382: R → T
         385-385: S → N
         388-388: D → S
         392-392: T → S
         398-398: V → F
         410-411: ET → DM
         415-420: NWAYNV → TWVDNF
         431-431: A → G
         443-443: L → M

    Show »
    Length:522
    Mass (Da):59,081
    Checksum:iC36A8CF1E84FBA87
    GO
    Isoform c (identifier: G5ECR8-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         370-371: FG → AI
         376-376: I → L
         382-382: R → T
         385-385: S → N
         388-388: D → S
         392-392: T → S
         398-398: V → F
         410-411: ET → DM
         415-420: NWAYNV → TWVDNF
         431-431: A → G
         443-443: L → M
         447-458: SKFLPRGSTRVF → RHVLATLDIMVQ
         459-522: Missing.

    Show »
    Length:458
    Mass (Da):52,212
    Checksum:iA177489059901221
    GO
    Isoform d (identifier: G5ECR8-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-18: MSRWKVVILCLLSFMFEI → MAFFSVRSSKIVFGLERSEEK

    Show »
    Length:525
    Mass (Da):59,320
    Checksum:i869C84AC63CD3A2A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 1818MSRWK…FMFEI → MAFFSVRSSKIVFGLERSEE K in isoform d. CuratedVSP_045725Add
    BLAST
    Alternative sequencei370 – 3712FG → AI in isoform b and isoform c. CuratedVSP_045726
    Alternative sequencei376 – 3761I → L in isoform b and isoform c. CuratedVSP_045727
    Alternative sequencei382 – 3821R → T in isoform b and isoform c. CuratedVSP_045728
    Alternative sequencei385 – 3851S → N in isoform b and isoform c. CuratedVSP_045729
    Alternative sequencei388 – 3881D → S in isoform b and isoform c. CuratedVSP_045730
    Alternative sequencei392 – 3921T → S in isoform b and isoform c. CuratedVSP_045731
    Alternative sequencei398 – 3981V → F in isoform b and isoform c. CuratedVSP_045732
    Alternative sequencei410 – 4112ET → DM in isoform b and isoform c. CuratedVSP_045733
    Alternative sequencei415 – 4206NWAYNV → TWVDNF in isoform b and isoform c. CuratedVSP_045734
    Alternative sequencei431 – 4311A → G in isoform b and isoform c. CuratedVSP_045735
    Alternative sequencei443 – 4431L → M in isoform b and isoform c. CuratedVSP_045736
    Alternative sequencei447 – 45812SKFLP…STRVF → RHVLATLDIMVQ in isoform c. CuratedVSP_045737Add
    BLAST
    Alternative sequencei459 – 52264Missing in isoform c. CuratedVSP_045738Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z81058, AL031254 Genomic DNA. Translation: CCD31056.1.
    Z81058, AL031254 Genomic DNA. Translation: CAB02924.1.
    Z81058, AL031254 Genomic DNA. Translation: CAC35813.1.
    Z81058 Genomic DNA. Translation: CAD42642.1.
    PIRiT18583.
    RefSeqiNP_001255965.1. NM_001269036.1. [G5ECR8-4]
    NP_503118.1. NM_070717.3. [G5ECR8-2]
    NP_503119.1. NM_070718.3. [G5ECR8-1]
    NP_741501.1. NM_171429.3. [G5ECR8-3]
    UniGeneiCel.13150.

    Genome annotation databases

    EnsemblMetazoaiF11E6.1a; F11E6.1a; WBGene00008706. [G5ECR8-1]
    GeneIDi178535.
    KEGGicel:CELE_F11E6.1.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z81058 , AL031254 Genomic DNA. Translation: CCD31056.1 .
    Z81058 , AL031254 Genomic DNA. Translation: CAB02924.1 .
    Z81058 , AL031254 Genomic DNA. Translation: CAC35813.1 .
    Z81058 Genomic DNA. Translation: CAD42642.1 .
    PIRi T18583.
    RefSeqi NP_001255965.1. NM_001269036.1. [G5ECR8-4 ]
    NP_503118.1. NM_070717.3. [G5ECR8-2 ]
    NP_503119.1. NM_070718.3. [G5ECR8-1 ]
    NP_741501.1. NM_171429.3. [G5ECR8-3 ]
    UniGenei Cel.13150.

    3D structure databases

    ProteinModelPortali G5ECR8.
    SMRi G5ECR8. Positions 25-521.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi G5ECR8. 1 interaction.

    Proteomic databases

    PRIDEi G5ECR8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai F11E6.1a ; F11E6.1a ; WBGene00008706 . [G5ECR8-1 ]
    GeneIDi 178535.
    KEGGi cel:CELE_F11E6.1.

    Organism-specific databases

    CTDi 178535.
    WormBasei F11E6.1a ; CE20669 ; WBGene00008706 ; gba-3.
    F11E6.1b ; CE26698 ; WBGene00008706 ; gba-3.
    F11E6.1c ; CE31166 ; WBGene00008706 ; gba-3.
    F11E6.1d ; CE46394 ; WBGene00008706 ; gba-3.

    Phylogenomic databases

    eggNOGi COG5520.
    GeneTreei ENSGT00390000009464.
    OMAi WNLCLDE.
    OrthoDBi EOG76DTRZ.
    PhylomeDBi G5ECR8.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .

    Miscellaneous databases

    NextBioi 901518.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR11069. PTHR11069. 1 hit.
    Pfami PF02055. Glyco_hydro_30. 1 hit.
    [Graphical view ]
    PRINTSi PR00843. GLHYDRLASE30.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiGLCM3_CAEEL
    AccessioniPrimary (citable) accession number: G5ECR8
    Secondary accession number(s): G5EEA6, G5EFK0, Q8MQ76
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2013
    Last sequence update: December 14, 2011
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3