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Protein

Putative glucosylceramidase 3

Gene

gba-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei259 – 2591Proton donorBy similarity
Active sitei364 – 3641NucleophileBy similarity

GO - Molecular functioni

  1. glucosylceramidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. sphingolipid metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glucosylceramidase 3 (EC:3.2.1.45)
Gene namesi
Name:gba-3
ORF Names:F11E6.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiF11E6.1a; CE20669; WBGene00008706; gba-3.
F11E6.1b; CE26698; WBGene00008706; gba-3.
F11E6.1c; CE31166; WBGene00008706; gba-3.
F11E6.1d; CE46394; WBGene00008706; gba-3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 522501Putative glucosylceramidase 3PRO_0000421455Add
BLAST

Proteomic databases

PRIDEiG5ECR8.

Interactioni

Protein-protein interaction databases

IntActiG5ECR8. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliG5ECR8.
SMRiG5ECR8. Positions 25-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5520.
GeneTreeiENSGT00390000009464.
InParanoidiG5ECR8.
KOiK01201.
OMAiWNLCLDE.
OrthoDBiEOG76DTRZ.
PhylomeDBiG5ECR8.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: G5ECR8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE
60 70 80 90 100
PIGEIAEGKA IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER
110 120 130 140 150
FQNIFGFGGA FTDSAGDQFV SLSETLQNYI VDSYFGKNGL EYNIGRVPIA
160 170 180 190 200
SCDFSTHEYS YDDVHDDFEL KHFALPDEDL KLKIPFIKKA IEKTEGNIQL
210 220 230 240 250
FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF FEAYSSHAIT
260 270 280 290 300
FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET
310 320 330 340 350
LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI
360 370 380 390 400
TQRHHPTKFI FGTEACAGYF GHHGPIMGDW FRAESYADDI ITDLNHHVTG
410 420 430 440 450
WTDWNLCLDE TGGPNWAYNV VDSPIIVNRT AQEFYKQPMF YALGHFSKFL
460 470 480 490 500
PRGSTRVFTK IEGNLAVSAT SVVIEGGRRA TVILSKASNS LLTRIVDSST
510 520
GFSIVLNLPP RSIHTVIWKK RK
Length:522
Mass (Da):59,088
Last modified:December 14, 2011 - v1
Checksum:i3CD73EE50CFBA7C7
GO
Isoform b (identifier: G5ECR8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: FG → AI
     376-376: I → L
     382-382: R → T
     385-385: S → N
     388-388: D → S
     392-392: T → S
     398-398: V → F
     410-411: ET → DM
     415-420: NWAYNV → TWVDNF
     431-431: A → G
     443-443: L → M

Show »
Length:522
Mass (Da):59,081
Checksum:iC36A8CF1E84FBA87
GO
Isoform c (identifier: G5ECR8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: FG → AI
     376-376: I → L
     382-382: R → T
     385-385: S → N
     388-388: D → S
     392-392: T → S
     398-398: V → F
     410-411: ET → DM
     415-420: NWAYNV → TWVDNF
     431-431: A → G
     443-443: L → M
     447-458: SKFLPRGSTRVF → RHVLATLDIMVQ
     459-522: Missing.

Show »
Length:458
Mass (Da):52,212
Checksum:iA177489059901221
GO
Isoform d (identifier: G5ECR8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MSRWKVVILCLLSFMFEI → MAFFSVRSSKIVFGLERSEEK

Show »
Length:525
Mass (Da):59,320
Checksum:i869C84AC63CD3A2A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1818MSRWK…FMFEI → MAFFSVRSSKIVFGLERSEE K in isoform d. CuratedVSP_045725Add
BLAST
Alternative sequencei370 – 3712FG → AI in isoform b and isoform c. CuratedVSP_045726
Alternative sequencei376 – 3761I → L in isoform b and isoform c. CuratedVSP_045727
Alternative sequencei382 – 3821R → T in isoform b and isoform c. CuratedVSP_045728
Alternative sequencei385 – 3851S → N in isoform b and isoform c. CuratedVSP_045729
Alternative sequencei388 – 3881D → S in isoform b and isoform c. CuratedVSP_045730
Alternative sequencei392 – 3921T → S in isoform b and isoform c. CuratedVSP_045731
Alternative sequencei398 – 3981V → F in isoform b and isoform c. CuratedVSP_045732
Alternative sequencei410 – 4112ET → DM in isoform b and isoform c. CuratedVSP_045733
Alternative sequencei415 – 4206NWAYNV → TWVDNF in isoform b and isoform c. CuratedVSP_045734
Alternative sequencei431 – 4311A → G in isoform b and isoform c. CuratedVSP_045735
Alternative sequencei443 – 4431L → M in isoform b and isoform c. CuratedVSP_045736
Alternative sequencei447 – 45812SKFLP…STRVF → RHVLATLDIMVQ in isoform c. CuratedVSP_045737Add
BLAST
Alternative sequencei459 – 52264Missing in isoform c. CuratedVSP_045738Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81058, AL031254 Genomic DNA. Translation: CCD31056.1.
Z81058, AL031254 Genomic DNA. Translation: CAB02924.1.
Z81058, AL031254 Genomic DNA. Translation: CAC35813.1.
Z81058 Genomic DNA. Translation: CAD42642.1.
PIRiT18583.
RefSeqiNP_001255965.1. NM_001269036.1. [G5ECR8-4]
NP_503118.1. NM_070717.3. [G5ECR8-2]
NP_503119.1. NM_070718.3. [G5ECR8-1]
NP_741501.1. NM_171429.3. [G5ECR8-3]
UniGeneiCel.13150.

Genome annotation databases

EnsemblMetazoaiF11E6.1a; F11E6.1a; WBGene00008706. [G5ECR8-1]
GeneIDi178535.
KEGGicel:CELE_F11E6.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81058, AL031254 Genomic DNA. Translation: CCD31056.1.
Z81058, AL031254 Genomic DNA. Translation: CAB02924.1.
Z81058, AL031254 Genomic DNA. Translation: CAC35813.1.
Z81058 Genomic DNA. Translation: CAD42642.1.
PIRiT18583.
RefSeqiNP_001255965.1. NM_001269036.1. [G5ECR8-4]
NP_503118.1. NM_070717.3. [G5ECR8-2]
NP_503119.1. NM_070718.3. [G5ECR8-1]
NP_741501.1. NM_171429.3. [G5ECR8-3]
UniGeneiCel.13150.

3D structure databases

ProteinModelPortaliG5ECR8.
SMRiG5ECR8. Positions 25-521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiG5ECR8. 1 interaction.

Proteomic databases

PRIDEiG5ECR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF11E6.1a; F11E6.1a; WBGene00008706. [G5ECR8-1]
GeneIDi178535.
KEGGicel:CELE_F11E6.1.

Organism-specific databases

CTDi178535.
WormBaseiF11E6.1a; CE20669; WBGene00008706; gba-3.
F11E6.1b; CE26698; WBGene00008706; gba-3.
F11E6.1c; CE31166; WBGene00008706; gba-3.
F11E6.1d; CE46394; WBGene00008706; gba-3.

Phylogenomic databases

eggNOGiCOG5520.
GeneTreeiENSGT00390000009464.
InParanoidiG5ECR8.
KOiK01201.
OMAiWNLCLDE.
OrthoDBiEOG76DTRZ.
PhylomeDBiG5ECR8.

Enzyme and pathway databases

UniPathwayiUPA00222.

Miscellaneous databases

NextBioi901518.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiGLCM3_CAEEL
AccessioniPrimary (citable) accession number: G5ECR8
Secondary accession number(s): G5EEA6, G5EFK0, Q8MQ76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: December 14, 2011
Last modified: January 7, 2015
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.