Putative glucosylceramidase 3 precursor

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G5ECR8 (GLCM3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 21. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative glucosylceramidase 3
EC=3.2.1.45

Gene names

Name:	gba-3
ORF Names:	F11E6.1

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	D-glucosyl-N-acylsphingosine + H₂O = D-glucose + N-acylsphingosine.
Pathway	Lipid metabolism; sphingolipid metabolism.
Sequence similarities	Belongs to the glycosyl hydrolase 30 family.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro sphingolipid metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	glucosylceramidase activity Inferred from electronic annotation. Source: UniProtKB-EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: G5ECR8-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: G5ECR8-2) The sequence of this isoform differs from the canonical sequence as follows: 370-371: FG → AI 376-376: I → L 382-382: R → T 385-385: S → N 388-388: D → S 392-392: T → S 398-398: V → F 410-411: ET → DM 415-420: NWAYNV → TWVDNF 431-431: A → G 443-443: L → M

Isoform c (identifier: G5ECR8-3) The sequence of this isoform differs from the canonical sequence as follows: 370-371: FG → AI 376-376: I → L 382-382: R → T 385-385: S → N 388-388: D → S 392-392: T → S 398-398: V → F 410-411: ET → DM 415-420: NWAYNV → TWVDNF 431-431: A → G 443-443: L → M 447-458: SKFLPRGSTRVF → RHVLATLDIMVQ 459-522: Missing.

Isoform d (identifier: G5ECR8-4) The sequence of this isoform differs from the canonical sequence as follows: 1-18: MSRWKVVILCLLSFMFEI → MAFFSVRSSKIVFGLERSEEK

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

Potential

Chain

22 – 522

501

Putative glucosylceramidase 3

PRO_0000421455

Sites

Active site

259

Proton donor By similarity

Active site

364

Nucleophile By similarity

Natural variations

Alternative sequence

1 – 18

MSRWK…FMFEI → MAFFSVRSSKIVFGLERSEE K in isoform d.

VSP_045725

Alternative sequence

370 – 371

FG → AI in isoform b and isoform c.

VSP_045726

Alternative sequence

376

I → L in isoform b and isoform c.

VSP_045727

Alternative sequence

382

R → T in isoform b and isoform c.

VSP_045728

Alternative sequence

385

S → N in isoform b and isoform c.

VSP_045729

Alternative sequence

388

D → S in isoform b and isoform c.

VSP_045730

Alternative sequence

392

T → S in isoform b and isoform c.

VSP_045731

Alternative sequence

398

V → F in isoform b and isoform c.

VSP_045732

Alternative sequence

410 – 411

ET → DM in isoform b and isoform c.

VSP_045733

Alternative sequence

415 – 420

NWAYNV → TWVDNF in isoform b and isoform c.

VSP_045734

Alternative sequence

431

A → G in isoform b and isoform c.

VSP_045735

Alternative sequence

443

L → M in isoform b and isoform c.

VSP_045736

Alternative sequence

447 – 458

SKFLP…STRVF → RHVLATLDIMVQ in isoform c.

VSP_045737

Alternative sequence

459 – 522

Missing in isoform c.

VSP_045738

Sequences

Sequence		Length	Mass (Da)
Isoform a [UniParc]. Last modified December 14, 2011. Version 1. Checksum: 3CD73EE50CFBA7C7 Show »	FASTA	522	59,088
10 20 30 40 50 60 MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE PIGEIAEGKA 70 80 90 100 110 120 IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER FQNIFGFGGA FTDSAGDQFV 130 140 150 160 170 180 SLSETLQNYI VDSYFGKNGL EYNIGRVPIA SCDFSTHEYS YDDVHDDFEL KHFALPDEDL 190 200 210 220 230 240 KLKIPFIKKA IEKTEGNIQL FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF 250 260 270 280 290 300 FEAYSSHAIT FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET 310 320 330 340 350 360 LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI TQRHHPTKFI 370 380 390 400 410 420 FGTEACAGYF GHHGPIMGDW FRAESYADDI ITDLNHHVTG WTDWNLCLDE TGGPNWAYNV 430 440 450 460 470 480 VDSPIIVNRT AQEFYKQPMF YALGHFSKFL PRGSTRVFTK IEGNLAVSAT SVVIEGGRRA 490 500 510 520 TVILSKASNS LLTRIVDSST GFSIVLNLPP RSIHTVIWKK RK « Hide
Isoform b [UniParc]. Checksum: C36A8CF1E84FBA87 Show »	FASTA	522	59,081
10 20 30 40 50 60 MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE PIGEIAEGKA 70 80 90 100 110 120 IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER FQNIFGFGGA FTDSAGDQFV 130 140 150 160 170 180 SLSETLQNYI VDSYFGKNGL EYNIGRVPIA SCDFSTHEYS YDDVHDDFEL KHFALPDEDL 190 200 210 220 230 240 KLKIPFIKKA IEKTEGNIQL FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF 250 260 270 280 290 300 FEAYSSHAIT FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET 310 320 330 340 350 360 LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI TQRHHPTKFI 370 380 390 400 410 420 FGTEACAGYA IHHGPLMGDW FTAENYASDI ISDLNHHFTG WTDWNLCLDD MGGPTWVDNF 430 440 450 460 470 480 VDSPIIVNRT GQEFYKQPMF YAMGHFSKFL PRGSTRVFTK IEGNLAVSAT SVVIEGGRRA 490 500 510 520 TVILSKASNS LLTRIVDSST GFSIVLNLPP RSIHTVIWKK RK « Hide
Isoform c [UniParc]. Checksum: A177489059901221 Show »	FASTA	458	52,212
10 20 30 40 50 60 MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE PIGEIAEGKA 70 80 90 100 110 120 IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER FQNIFGFGGA FTDSAGDQFV 130 140 150 160 170 180 SLSETLQNYI VDSYFGKNGL EYNIGRVPIA SCDFSTHEYS YDDVHDDFEL KHFALPDEDL 190 200 210 220 230 240 KLKIPFIKKA IEKTEGNIQL FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF 250 260 270 280 290 300 FEAYSSHAIT FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET 310 320 330 340 350 360 LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI TQRHHPTKFI 370 380 390 400 410 420 FGTEACAGYA IHHGPLMGDW FTAENYASDI ISDLNHHFTG WTDWNLCLDD MGGPTWVDNF 430 440 450 VDSPIIVNRT GQEFYKQPMF YAMGHFRHVL ATLDIMVQ « Hide
Isoform d [UniParc]. Checksum: 869C84AC63CD3A2A Show »	FASTA	525	59,320
10 20 30 40 50 60 MAFFSVRSSK IVFGLERSEE KGHASFQCNP KTYDGAFLNI VCVCNATFCD EIEPIGEIAE 70 80 90 100 110 120 GKAIVYRSSL DGDRLKRMSM KMKEKLRKNE SVNVTITIDA SERFQNIFGF GGAFTDSAGD 130 140 150 160 170 180 QFVSLSETLQ NYIVDSYFGK NGLEYNIGRV PIASCDFSTH EYSYDDVHDD FELKHFALPD 190 200 210 220 230 240 EDLKLKIPFI KKAIEKTEGN IQLFASPWSA PGWMKVTGRM RGGGAMRNDK RVYQAYADYF 250 260 270 280 290 300 FKFFEAYSSH AITFWGLTIQ NEPSTGADMA WRWQTMNYTA ETMRDFLKKY LGPKLKENKL 310 320 330 340 350 360 TETLKVMVLD DGRGLLPGWA DTIFNDPEAT KYADGVAVHW YGNLYSPAVL LDITQRHHPT 370 380 390 400 410 420 KFIFGTEACA GYFGHHGPIM GDWFRAESYA DDIITDLNHH VTGWTDWNLC LDETGGPNWA 430 440 450 460 470 480 YNVVDSPIIV NRTAQEFYKQ PMFYALGHFS KFLPRGSTRV FTKIEGNLAV SATSVVIEGG 490 500 510 520 RRATVILSKA SNSLLTRIVD SSTGFSIVLN LPPRSIHTVI WKKRK « Hide

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z81058, AL031254 Genomic DNA. Translation: CCD31056.1. Z81058, AL031254 Genomic DNA. Translation: CAB02924.1. Z81058, AL031254 Genomic DNA. Translation: CAC35813.1. Z81058 Genomic DNA. Translation: CAD42642.1.
PIR	T18583.
RefSeq	NP_001255965.1. NM_001269036.1. NP_503118.1. NM_070717.3. NP_503119.1. NM_070718.3. NP_741501.1. NM_171429.3.
UniGene	Cel.13150.
3D structure databases
ProteinModelPortal	G5ECR8.
SMR	G5ECR8. Positions 25-521.
ModBase	Search...
Protein-protein interaction databases
IntAct	G5ECR8. 1 interaction.
Proteomic databases
PRIDE	G5ECR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F11E6.1a; F11E6.1a; F11E6.1.
GeneID	178535.
KEGG	cel:CELE_F11E6.1.
UCSC	F11E6.1a. c. elegans.
Organism-specific databases
CTD	178535.
WormBase	F11E6.1a; CE20669; WBGene00008706; gba-3. F11E6.1b; CE26698; WBGene00008706; gba-3. F11E6.1c; CE31166; WBGene00008706; gba-3. F11E6.1d; CE46394; WBGene00008706; gba-3.
Phylogenomic databases
eggNOG	COG5520.
GeneTree	ENSGT00390000009464.
OMA	WNLCLDE.
OrthoDB	EOG76DTRZ.
Enzyme and pathway databases
UniPathway	UPA00222.
Gene expression databases
ArrayExpress	G5ECR8.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001139. Glyco_hydro_30. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR11069. PTHR11069. 1 hit.
Pfam	PF02055. Glyco_hydro_30. 1 hit. [Graphical view]
PRINTS	PR00843. GLHYDRLASE30.
SUPFAM	SSF51445. SSF51445. 1 hit.
ProtoNet	Search...
Other
NextBio	901518.

Entry information

Entry name

GLCM3_CAEEL

Accession

Primary (citable) accession number: G5ECR8
Secondary accession number(s): G5EEA6, G5EFK0, Q8MQ76

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2013
Last sequence update:	December 14, 2011
Last modified:	November 13, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents