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Protein

Putative glucosylceramidase 3

Gene

gba-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei259Proton donorBy similarity1
Active sitei364NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00222

Protein family/group databases

CAZyiGH30 Glycoside Hydrolase Family 30

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glucosylceramidase 3 (EC:3.2.1.45)
Gene namesi
Name:gba-3
ORF Names:F11E6.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF11E6.1a ; CE20669 ; WBGene00008706 ; gba-3
F11E6.1b ; CE26698 ; WBGene00008706 ; gba-3
F11E6.1c ; CE31166 ; WBGene00008706 ; gba-3
F11E6.1d ; CE46394 ; WBGene00008706 ; gba-3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000042145522 – 522Putative glucosylceramidase 3Add BLAST501

Proteomic databases

PaxDbiG5ECR8
PeptideAtlasiG5ECR8

Expressioni

Gene expression databases

BgeeiWBGene00008706

Interactioni

Protein-protein interaction databases

BioGridi436101 interactor.
IntActiG5ECR8 1 interactor.
MINTiG5ECR8
STRINGi6239.F11E6.1d

Structurei

3D structure databases

ProteinModelPortaliG5ECR8
SMRiG5ECR8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2566 Eukaryota
COG5520 LUCA
GeneTreeiENSGT00390000009464
HOGENOMiHOG000270031
InParanoidiG5ECR8
KOiK01201
OMAiPIMGDWF
OrthoDBiEOG091G06I4
PhylomeDBiG5ECR8

Family and domain databases

Gene3Di2.60.40.11802 hits
InterProiView protein in InterPro
IPR033452 GH30_C
IPR001139 Glyco_hydro_30
IPR033453 Glyco_hydro_30_TIM-barrel
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR11069 PTHR11069, 1 hit
PfamiView protein in Pfam
PF02055 Glyco_hydro_30, 1 hit
PF17189 Glyco_hydro_30C, 1 hit
PRINTSiPR00843 GLHYDRLASE30
SUPFAMiSSF51445 SSF51445, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: G5ECR8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRWKVVILC LLSFMFEIGH ASFQCNPKTY DGAFLNIVCV CNATFCDEIE
60 70 80 90 100
PIGEIAEGKA IVYRSSLDGD RLKRMSMKMK EKLRKNESVN VTITIDASER
110 120 130 140 150
FQNIFGFGGA FTDSAGDQFV SLSETLQNYI VDSYFGKNGL EYNIGRVPIA
160 170 180 190 200
SCDFSTHEYS YDDVHDDFEL KHFALPDEDL KLKIPFIKKA IEKTEGNIQL
210 220 230 240 250
FASPWSAPGW MKVTGRMRGG GAMRNDKRVY QAYADYFFKF FEAYSSHAIT
260 270 280 290 300
FWGLTIQNEP STGADMAWRW QTMNYTAETM RDFLKKYLGP KLKENKLTET
310 320 330 340 350
LKVMVLDDGR GLLPGWADTI FNDPEATKYA DGVAVHWYGN LYSPAVLLDI
360 370 380 390 400
TQRHHPTKFI FGTEACAGYF GHHGPIMGDW FRAESYADDI ITDLNHHVTG
410 420 430 440 450
WTDWNLCLDE TGGPNWAYNV VDSPIIVNRT AQEFYKQPMF YALGHFSKFL
460 470 480 490 500
PRGSTRVFTK IEGNLAVSAT SVVIEGGRRA TVILSKASNS LLTRIVDSST
510 520
GFSIVLNLPP RSIHTVIWKK RK
Length:522
Mass (Da):59,088
Last modified:December 14, 2011 - v1
Checksum:i3CD73EE50CFBA7C7
GO
Isoform b (identifier: G5ECR8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: FG → AI
     376-376: I → L
     382-382: R → T
     385-385: S → N
     388-388: D → S
     392-392: T → S
     398-398: V → F
     410-411: ET → DM
     415-420: NWAYNV → TWVDNF
     431-431: A → G
     443-443: L → M

Show »
Length:522
Mass (Da):59,081
Checksum:iC36A8CF1E84FBA87
GO
Isoform c (identifier: G5ECR8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     370-371: FG → AI
     376-376: I → L
     382-382: R → T
     385-385: S → N
     388-388: D → S
     392-392: T → S
     398-398: V → F
     410-411: ET → DM
     415-420: NWAYNV → TWVDNF
     431-431: A → G
     443-443: L → M
     447-458: SKFLPRGSTRVF → RHVLATLDIMVQ
     459-522: Missing.

Show »
Length:458
Mass (Da):52,212
Checksum:iA177489059901221
GO
Isoform d (identifier: G5ECR8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MSRWKVVILCLLSFMFEI → MAFFSVRSSKIVFGLERSEEK

Show »
Length:525
Mass (Da):59,320
Checksum:i869C84AC63CD3A2A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0457251 – 18MSRWK…FMFEI → MAFFSVRSSKIVFGLERSEE K in isoform d. CuratedAdd BLAST18
Alternative sequenceiVSP_045726370 – 371FG → AI in isoform b and isoform c. Curated2
Alternative sequenceiVSP_045727376I → L in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045728382R → T in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045729385S → N in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045730388D → S in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045731392T → S in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045732398V → F in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045733410 – 411ET → DM in isoform b and isoform c. Curated2
Alternative sequenceiVSP_045734415 – 420NWAYNV → TWVDNF in isoform b and isoform c. Curated6
Alternative sequenceiVSP_045735431A → G in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045736443L → M in isoform b and isoform c. Curated1
Alternative sequenceiVSP_045737447 – 458SKFLP…STRVF → RHVLATLDIMVQ in isoform c. CuratedAdd BLAST12
Alternative sequenceiVSP_045738459 – 522Missing in isoform c. CuratedAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81058, AL031254 Genomic DNA Translation: CCD31056.1
Z81058, AL031254 Genomic DNA Translation: CAB02924.1
Z81058, AL031254 Genomic DNA Translation: CAC35813.1
Z81058 Genomic DNA Translation: CAD42642.1
PIRiT18583
RefSeqiNP_001255965.1, NM_001269036.1 [G5ECR8-4]
NP_503118.1, NM_070717.3 [G5ECR8-2]
NP_503119.1, NM_070718.3 [G5ECR8-1]
UniGeneiCel.13150

Genome annotation databases

EnsemblMetazoaiF11E6.1a; F11E6.1a; WBGene00008706 [G5ECR8-1]
GeneIDi178535
KEGGicel:CELE_F11E6.1
UCSCiF11E6.1a c. elegans

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLCM3_CAEEL
AccessioniPrimary (citable) accession number: G5ECR8
Secondary accession number(s): G5EEA6, G5EFK0, Q8MQ76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: December 14, 2011
Last modified: February 28, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome