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Protein

Frizzled-2

Gene

cfz-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for Wnt proteins (PubMed:19855022, PubMed:20711352). Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of gsk-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of gsk-3 kinase. Both pathways seem to involve interactions with G-proteins (Probable). Required for the migration and axon formation and guidance of different neuronal cell types including canal-associated neurons (CAN), hermaphrodite-specific neurons (HSN), anterior lateral microtubule neurons (ALM), and the right Q neuroblast (QR) and its descendants (PubMed:16109397). Directs ALM migration through frizzled protein mom-5 and Wnt ligands cwn-1, cwn-2 and egl-20 (PubMed:16109397). May act redundantly with mom-5 to direct CAN migration (PubMed:16109397). Plays a role in the organization of head ganglion cells (PubMed:16109397). Probably by acting as a receptor for Wnt ligand cwn-2, plays a role in the positioning of the nerve ring and may in addition positively regulate the neurite outgrowth of RME GABAergic motor neurons along the anterior-posterior axis of the body (PubMed:19855022, PubMed:20711352).Curated3 Publications

GO - Molecular functioni

GO - Biological processi

  • canonical Wnt signaling pathway Source: GO_Central
  • motor neuron migration Source: UniProtKB
  • neuroblast migration Source: UniProtKB
  • neuron migration Source: UniProtKB
  • non-canonical Wnt signaling pathway Source: GO_Central
  • positive regulation of motor neuron migration Source: UniProtKB
  • sensory neuron migration Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, G-protein coupled receptor, Receptor, Transducer
Biological processNeurogenesis, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-CEL-4086398. Ca2+ pathway.
R-CEL-4608870. Asymmetric localization of PCP proteins.
R-CEL-5140745. WNT5A-dependent internalization of FZD2, FZD5 and ROR2.

Names & Taxonomyi

Protein namesi
Recommended name:
Frizzled-2Imported
Gene namesi
Name:cfz-2Imported
ORF Names:F27E11.3Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF27E11.3; CE39149; WBGene00000478; cfz-2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 236ExtracellularCuratedAdd BLAST219
Transmembranei237 – 257Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini258 – 268CytoplasmicCuratedAdd BLAST11
Transmembranei269 – 289Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini290 – 312ExtracellularCuratedAdd BLAST23
Transmembranei313 – 333Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini334 – 354CytoplasmicCuratedAdd BLAST21
Transmembranei355 – 375Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini376 – 398ExtracellularCuratedAdd BLAST23
Transmembranei399 – 419Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini420 – 449CytoplasmicCuratedAdd BLAST30
Transmembranei450 – 470Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini471 – 497ExtracellularCuratedAdd BLAST27
Transmembranei498 – 518Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini519 – 578CytoplasmicCuratedAdd BLAST60

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Displaced anterior nerve ring and neuronal cell migration defects including irregular localization of canal-associated neurons (CAN), hermaphrodite-specific neurons (HSN), anterior lateral microtubule neurons (ALM), and the right (but not the left) Q neuroblast (QR) and its descendants (PubMed:16109397, PubMed:9851916). Axon growth and guidance defects whereby some HSN, male CP neurons and CAN extend ectopic axons or branches, and furthermore some HSN irregularly route towards the ventral midline of the body (PubMed:16109397). Disorganized cells of the anterior ganglion which are normally compacted together when they form the head ganglion in wild-type animals (PubMed:16109397). Double knockout with mom-5 or cwn-2 results in enhanced CAN migration defects (PubMed:16109397). Double knockout with Wnt ligands cwn-1, cwn-2, egl-20 or frizzled protein mom-5 rescues the ALM migration defects in the single cfz-2 knockout (PubMed:16109397). Triple knockout with cwn-1 and cwn-2 results in enhanced neuronal cell migratory defects (PubMed:16109397).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_500766123818 – 578Frizzled-2CuratedAdd BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi25 ↔ 86PROSITE-ProRule annotation
Disulfide bondi33 ↔ 79PROSITE-ProRule annotation
Glycosylationi39N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi70 ↔ 108PROSITE-ProRule annotation
Disulfide bondi97 ↔ 141PROSITE-ProRule annotation
Disulfide bondi101 ↔ 125PROSITE-ProRule annotation
Glycosylationi213N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiG5ECQ2.
PaxDbiG5ECQ2.
PeptideAtlasiG5ECQ2.

Expressioni

Tissue specificityi

Expressed in two pairs of head neurons and throughout the pharynx.1 Publication

Developmental stagei

First expressed at the 100-cell stage of embryogenesis. During embryonic elongation, expressed in anterior, posterior and midbody cells of the embryo and in cellular projections. At hatching, expression is restricted to a few cells in the head and a pair of cells in the tail.1 Publication

Gene expression databases

BgeeiWBGene00000478.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiG5ECQ2. 11 interactors.
STRINGi6239.F27E11.3a.

Structurei

3D structure databases

ProteinModelPortaliG5ECQ2.
SMRiG5ECQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 144FZPROSITE-ProRule annotationAdd BLAST125

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi522 – 527Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family membersBy similarity6
Motifi556 – 558PDZ-bindingSequence analysis3

Domaini

Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.By similarity
The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3577. Eukaryota.
ENOG410XRC8. LUCA.
GeneTreeiENSGT00760000118864.
HOGENOMiHOG000233236.
KOiK02375.
OMAiGKNSKNC.
OrthoDBiEOG091G0N5M.
PhylomeDBiG5ECQ2.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiView protein in InterPro
IPR015526. Frizzled/SFRP.
IPR000539. Frizzled/Smoothened_TM.
IPR020067. Frizzled_dom.
IPR036790. Frizzled_dom_sf.
IPR017981. GPCR_2-like.
PANTHERiPTHR11309. PTHR11309. 1 hit.
PfamiView protein in Pfam
PF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
PRINTSiPR00489. FRIZZLED.
SMARTiView protein in SMART
SM00063. FRI. 1 hit.
SM01330. Frizzled. 1 hit.
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiView protein in PROSITE
PS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G5ECQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRISVLFL LLGSCGALFG KRQKCEQITI PLCKGIGYNM TSFPNSYGHE
60 70 80 90 100
KQEEAGLEVH QFYPLVEVGC FQHLKFFLCT MYTPICQENY DKPILPCMEL
110 120 130 140 150
CVEARSKCSP IMAKYGFRWP ETLSCEALPK MSDQMSTGNI CAAPPDTPKK
160 170 180 190 200
QHKGHHHKNQ NQNQNQNHNY SPDGPEVGIS KIDNEVIAGP SECQCTCNQP
210 220 230 240 250
FQFVASEKSK VGNVTNCAYS CHSPALAESH SLVSNWMAFW SITCCVLASF
260 270 280 290 300
TFLTFLIETD RFQYPERPIF MLAFCQLMVA VGFMIRYFVG HEEIACDSMR
310 320 330 340 350
IKGADDNSGS LCFVVFLLTY FFGMAASVWW VILSLTWVLS AASKWSPEAI
360 370 380 390 400
SSFSFHFHVV GWCLPAIQTV LVIVFNAIDG DPITGICYVG NTDLQFQRIF
410 420 430 440 450
VLFPLLVYFI VGVLFLVIGF CNLWSIRNEV QKQHPSLESA HKITQLMSKI
460 470 480 490 500
GIFSLLYTIP SLLIICVLFY EQNHRSLWEQ SQLCSCSPKQ TIGDSSLIIS
510 520 530 540 550
LIKTCCMCIL GWTSGFWVCS TKTLSSWKNA ICCLGSSRSL PKYQPADILY
560 570
AKSDMSSSQF YNTSLRHNHL YGGIPDKL
Length:578
Mass (Da):65,025
Last modified:December 14, 2011 - v1
Checksum:i3B2A3666A8DB3596
GO

Sequence cautioni

G5ECQ2: The sequence BAA84678 differs from that shown. Reason: Erroneous gene model prediction.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ178242 mRNA. Translation: ABA18181.1.
BX284605 Genomic DNA. Translation: CCD62501.1.
AB026113 Genomic DNA. Translation: BAA84678.1. Sequence problems.
PIRiT37325.
RefSeqiNP_503965.2. NM_071564.5.
UniGeneiCel.6721.

Genome annotation databases

EnsemblMetazoaiF27E11.3; F27E11.3; WBGene00000478.
GeneIDi178768.
KEGGicel:CELE_F27E11.3.

Similar proteinsi

Entry informationi

Entry nameiFRIZ2_CAEEL
AccessioniPrimary (citable) accession number: G5ECQ2
Secondary accession number(s): Q9U8U6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2016
Last sequence update: December 14, 2011
Last modified: October 25, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families