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Protein

Tyrosine-protein kinase csk-1

Gene

csk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-receptor tyrosine-protein kinase which plays a role in pharynx function by regulating pumping and the orientation of pharyngeal muscle fibers, independently of src-1 and src-2 (PubMed:19210548). May phosphorylate and thereby negatively regulate src-1 and src-2 activities (PubMed:12527374).2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei310ATPPROSITE-ProRule annotation1
Active sitei403Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi289 – 297ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  • protein tyrosine kinase activity Source: UniProtKB

GO - Biological processi

  • embryo development ending in birth or egg hatching Source: WormBase
  • larval feeding behavior Source: WormBase
  • muscle fiber development Source: WormBase
  • nematode larval development Source: WormBase
  • pharyngeal pumping Source: WormBase

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-202427 Phosphorylation of CD3 and TCR zeta chains
R-CEL-354192 Integrin alphaIIb beta3 signaling
R-CEL-8863795 Downregulation of ERBB2 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase csk-1Curated (EC:2.7.10.21 Publication)
Alternative name(s):
C-terminal src kinaseCurated
Gene namesi
Name:csk-1Imported
ORF Names:Y48G1C.2Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiY48G1C.2a ; CE34405 ; WBGene00000812 ; csk-1
Y48G1C.2b ; CE49183 ; WBGene00000812 ; csk-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi310K → M: Loss of kinase activity. Reduction in src-1 and src-2-mediated tyrosine phosphorylation. Mutants arrest at L1 larval stage. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004345041 – 539Tyrosine-protein kinase csk-1CuratedAdd BLAST539

Proteomic databases

EPDiG5ECJ6
PaxDbiG5ECJ6
PeptideAtlasiG5ECJ6

Expressioni

Tissue specificityi

Expressed predominantly in pharyngeal muscles in procorpus, metacorpus and terminal bulb (PubMed:12527374, PubMed:19210548). Expressed also in some neurons (ASE, ADF, AVA, AUA, RMDV and BAG) in the head region, anchor cell, vulva, cells around anus, body wall muscle and gondal distal tip cells (PubMed:12527374).2 Publications

Developmental stagei

Expressed mainly in the pharynx in the loop stage embryo. At L1 larval stage, predominantly expressed in the pharynx with some expression in body wall muscle, anchor cells and tail region.1 Publication

Gene expression databases

BgeeiWBGene00000812
ExpressionAtlasiG5ECJ6 baseline

Interactioni

Protein-protein interaction databases

IntActiG5ECJ6, 1 interactor
STRINGi6239.Y48G1C.2.1

Structurei

3D structure databases

ProteinModelPortaliG5ECJ6
SMRiG5ECJ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 110SH3PROSITE-ProRule annotationAdd BLAST68
Domaini151 – 241SH2PROSITE-ProRule annotationAdd BLAST91
Domaini283 – 535Protein kinasePROSITE-ProRule annotationAdd BLAST253

Sequence similaritiesi

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG0197 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00760000119011
KOiK05728
OMAiWALNMKD
OrthoDBiEOG091G05PB
PhylomeDBiG5ECJ6

Family and domain databases

CDDicd09937 SH2_csk_like, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR035026 CSK
IPR035027 Csk-like_SH2
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
PANTHERiPTHR24418:SF307 PTHR24418:SF307, 1 hit
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
PF00017 SH2, 1 hit
PRINTSiPR00401 SH2DOMAIN
PR00109 TYRKINASE
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF55550 SSF55550, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS50001 SH2, 1 hit
PS50002 SH3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform aImported (identifier: G5ECJ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSNGNSYNHH HQFPMSIPIS CSSHSIQSQS RMNTLNANRD LLSPGNDVIV
60 70 80 90 100
TRTVSPSFYS HGMPARDNVF RKDDHVRILG NTTDPAWYRA RNANQEEGLV
110 120 130 140 150
HADCVVRING QAYDNGIVRM RASGCDVAPG AASTTSSTSS HHSTAANHQP
160 170 180 190 200
WFHSMISREN TEKLLRGKPD GTFLVRESTN FPGDFTLCMS FHGKVEHYRI
210 220 230 240 250
EQTSGGQLTC DKEEYFSNLT QLVSHYKRDA DGLCHRLVTP IICETATFSS
260 270 280 290 300
NGSSSFGSSS TVDLEDRTSV FRHAGLVISS NDIDVGDTIG HGEFGDVRLG
310 320 330 340 350
TYKNRKVALK VSKRHGNGML DSLLDEAKFM VGLSHPNLVT LVGVVLDDVN
360 370 380 390 400
VYMITEYMAN GNLIDLLRSR GRHALERRQL MMFAMDICQG MCYLESKQIV
410 420 430 440 450
HRDLAARNVL LDDDLVAKVS DFGLAKKANS QSHDSASGKF PIKWTAPEAL
460 470 480 490 500
RHSQFTTKSD VWSFGILLWE IFSFGRVPYP RIPIQDVVRY IEKGYRMEAP
510 520 530
EGCPPEIFKV MNETWALSAQ DRPSFGQVLQ RLTTIRNTV
Length:539
Mass (Da):60,157
Last modified:December 14, 2011 - v1
Checksum:i35737ADE05B10C1E
GO
Isoform bImported (identifier: G5ECJ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MPIFSASRSR...DFIANGILKM

Note: No experimental confirmation available.Curated
Show »
Length:624
Mass (Da):69,914
Checksum:i9980A7D158AA3251
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0579361M → MPIFSASRSRSHHRNSRNPT KFSNFSIEKRSHSLGGSSSS PTSSFSSNDEFDDRQNKNKF WRQRNVSNAEIERIIEDFIA NGILKM in isoform b. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB096875 mRNA Translation: BAC76831.1
BX284601 Genomic DNA Translation: CCD71723.1
BX284601 Genomic DNA Translation: CDK13329.1
RefSeqiNP_001021778.1, NM_001026607.2 [G5ECJ6-1]
NP_001293162.1, NM_001306233.1 [G5ECJ6-2]
UniGeneiCel.5849

Genome annotation databases

EnsemblMetazoaiY48G1C.2a.1; Y48G1C.2a.1; WBGene00000812 [G5ECJ6-1]
Y48G1C.2a.2; Y48G1C.2a.2; WBGene00000812 [G5ECJ6-1]
GeneIDi266817
KEGGicel:CELE_Y48G1C.2

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCSK1_CAEEL
AccessioniPrimary (citable) accession number: G5ECJ6
Secondary accession number(s): V6CL92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: December 14, 2011
Last modified: March 28, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health