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Protein

Ceramide glucosyltransferase 2

Gene

cgt-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide.2 Publications

Catalytic activityi

UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine.2 Publications

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.2 Publications
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei285Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

ReactomeiR-CEL-1660662 Glycosphingolipid metabolism
UniPathwayiUPA00222

Protein family/group databases

CAZyiGT21 Glycosyltransferase Family 21

Chemistry databases

SwissLipidsiSLP:000000022

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide glucosyltransferase 2 (EC:2.4.1.80)
Gene namesi
Name:cgt-2
ORF Names:F20B4.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiF20B4.6 ; CE30960 ; WBGene00017625 ; cgt-2

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21
Transmembranei398 – 418HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced brood size.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004212821 – 443Ceramide glucosyltransferase 2Add BLAST443

Proteomic databases

PaxDbiG5EC84
PeptideAtlasiG5EC84
PRIDEiG5EC84

Expressioni

Gene expression databases

BgeeiWBGene00017625

Interactioni

Protein-protein interaction databases

BioGridi466612 interactors.
IntActiG5EC84 2 interactors.
STRINGi6239.F20B4.6

Structurei

3D structure databases

ProteinModelPortaliG5EC84
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi139D1Curated1
Motifi191D2Curated1
Motifi285D3Curated1
Motifi321 – 325(Q/R)XXRWCurated5

Domaini

The D1, D2, D3, (Q/R)XXRW motif is a critical part of the GCS active site, involved in catalysis and UDP-sugar binding.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2547 Eukaryota
COG1215 LUCA
GeneTreeiENSGT00390000012898
InParanoidiG5EC84
KOiK00720
OrthoDBiEOG091G0G3M
PhylomeDBiG5EC84

Family and domain databases

Gene3Di3.90.550.101 hit
InterProiView protein in InterPro
IPR025993 Ceramide_glucosylTrfase
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF13506 Glyco_transf_21, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

G5EC84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDKITGLEV EIIPEISEVQ PESLQEQNAS SLYYPLFSQY IPLLLTGTKK
60 70 80 90 100
ILEDIDYCTC FAVFGVVFVS ALYFLHIVGL CYGKYRLHRP TKPNPSLPGV
110 120 130 140 150
SIIKPIIGAD ANLYTNLETF FTTQYHKFEL LFCFDRSDDP AVKVVESLVK
160 170 180 190 200
KYPSVDSTMF FGGEKIGLNP KINNMMPAYR IAKYQLIMIS DSGIFMKSDA
210 220 230 240 250
VLDMASTMMS HETMALVTQT PYCKDRKGFA SVFEQIYFGT SHARIYLAGN
260 270 280 290 300
CLQFNCPTGM SSMMKKEALD ECGGFAAFSG YLAEDYFFGK KLASRGYKSG
310 320 330 340 350
ISTHPALQNS AAVTMTSFTD RVCRWVKLRM AMMPQIIFVE PLQDCFPSAL
360 370 380 390 400
IISFSLNYIA NIDMLTTIML HVVFWITMDC MVMCKMQNKK MSFSPLKFLL
410 420 430 440
IWLLRELFAP LVFIKAALDP SIRWRDNVFH LAWGGKIRSQ TSI
Length:443
Mass (Da):50,121
Last modified:December 14, 2011 - v1
Checksum:i2690419210CA6C93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF364401 mRNA Translation: AAK73018.1
FO081185 Genomic DNA Translation: CCD69753.1
PIRiT28827
RefSeqiNP_510857.2, NM_078456.4
UniGeneiCel.19350

Genome annotation databases

EnsemblMetazoaiF20B4.6; F20B4.6; WBGene00017625
GeneIDi181790
KEGGicel:CELE_F20B4.6

Similar proteinsi

Entry informationi

Entry nameiCGT2_CAEEL
AccessioniPrimary (citable) accession number: G5EC84
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: December 14, 2011
Last modified: March 28, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome