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Protein

6-phosphogluconate dehydrogenase, NAD(+)-dependent, decarboxylating

Gene

GOX1705

Organism
Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NAD to NADH.1 Publication

Catalytic activityi

6-phospho-D-gluconate + NAD+ = D-ribulose 5-phosphate + CO2 + NADH.1 Publication

Kineticsi

kcat is 23 sec(-1). Can use NAD or NADP, but has a clear preference for NAD in vivo.1 Publication
  1. KM=0.44 mM for 6-phosphogluconate1 Publication
  2. KM=64 µM for NAD+1 Publication
  3. KM=440 µM for NADP+1 Publication
  1. Vmax=41.7 µmol/min/mg enzyme1 Publication

Pathwayi: pentose phosphate pathway

This protein is involved in the pathway pentose phosphate pathway, which is part of Carbohydrate degradation.1 Publication
View all proteins of this organism that are known to be involved in the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101NADBy similarity1
Binding sitei101SubstrateBy similarity1
Binding sitei127SubstrateBy similarity1
Active sitei197Proton acceptorBy similarity1
Active sitei204Proton donorBy similarity1
Binding sitei205SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 12NADBy similarity6
Nucleotide bindingi30 – 32NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGluconate utilization
LigandNAD

Enzyme and pathway databases

BioCyciGOXY290633:GHB3-1703-MONOMER.
BRENDAi1.1.1.343. 38.
UniPathwayiUPA00115.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphogluconate dehydrogenase, NAD(+)-dependent, decarboxylatingCurated (EC:1.1.1.3431 Publication)
Short name:
6PGDH1 Publication
Gene namesi
Ordered Locus Names:GOX1705Imported
OrganismiGluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Taxonomic identifieri290633 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconobacter
Proteomesi
  • UP000006375 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004344361 – 3326-phosphogluconate dehydrogenase, NAD(+)-dependent, decarboxylatingAdd BLAST332

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi290633.GOX1705.

Structurei

3D structure databases

ProteinModelPortaliG5EBD7.
SMRiG5EBD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni127 – 129Substrate bindingBy similarity3
Regioni200 – 201Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108J5G. Bacteria.
COG1023. LUCA.
KOiK00033.
OMAiENGYGLM.
OrthoDBiPOG091H01QF.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
InterProiView protein in InterPro
IPR008927. 6-PGluconate_DH_C-like.
IPR004849. 6DGDH_YqeC.
IPR013328. 6PGD_dom_2.
IPR006114. 6PGDH_C.
IPR006115. 6PGDH_NADP-bd.
IPR016040. NAD(P)-bd_dom.
IPR006183. Pgluconate_DH.
PfamiView protein in Pfam
PF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
PRINTSiPR00076. 6PGDHDRGNASE.
SMARTiView protein in SMART
SM01350. 6PGD. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00872. gnd_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

G5EBD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIGIIGLGR MGGNIAVRLT RHGHDVVVHD RTSEVTTSVV GRCEAGRATP
60 70 80 90 100
ADTLADMAKL LEGDEHRVVW VMLPAGAITE DCVQQLGGLL GRGDIIIDGG
110 120 130 140 150
NTYYKDDVRR SAELAEKGIS YVDVGTSGGV WGLERGYCMM FGGTKETAEY
160 170 180 190 200
IDPILSALAP GIGDVPRTPG RDEAGHDPRA EQGYLHCGPA GSGHFVKMVH
210 220 230 240 250
NGIEYGMMQA FAEGFDIMKS KNSPILAEKD RFELNMGDIA EVWRRGSVVS
260 270 280 290 300
SWLLDLTAEA LTRSETLNEF SGEVADSGEG RWTIEAAIEE DVPAPVMTAA
310 320 330
LFTRFRSRSG NNFAEKILSA QRFGFGGHVE KK
Length:332
Mass (Da):35,876
Last modified:December 14, 2011 - v1
Checksum:i2D7466AEF76E8B08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000009 Genomic DNA. Translation: AAW61445.1.
RefSeqiWP_011253227.1. NC_006677.1.

Genome annotation databases

EnsemblBacteriaiAAW61445; AAW61445; GOX1705.
GeneIDi29628808.
KEGGigox:GOX1705.

Similar proteinsi

Entry informationi

Entry namei6PGDH_GLUOX
AccessioniPrimary (citable) accession number: G5EBD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: December 14, 2011
Last modified: June 7, 2017
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families