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G5EB45

- G5EB45_EMENI

UniProt

G5EB45 - G5EB45_EMENI

Protein

Alpha-amylase

Gene

AN2018.2

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 21 (01 Oct 2014)
      Sequence version 1 (14 Dec 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi134 – 1341Calcium 1
    Metal bindingi175 – 1751Calcium 1; via carbonyl oxygen
    Metal bindingi188 – 1881Calcium 1
    Active sitei219 – 2191NucleophileUniRule annotation
    Metal bindingi219 – 2191Calcium 2
    Metal bindingi223 – 2231Calcium 1; via carbonyl oxygen
    Active sitei243 – 2431Proton donorUniRule annotation
    Metal bindingi243 – 2431Calcium 2
    Sitei310 – 3101Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. alpha-amylase activity Source: UniProtKB-EC
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. carbohydrate catabolic process Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseUniRule annotation, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolismUniRule annotation

    Keywords - Ligandi

    CalciumUniRule annotation, Metal-bindingUniRule annotation

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
    Gene namesi
    ORF Names:AN2018.2Imported, ANIA_02018Imported
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)Imported
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome VII

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi163 ↔ 177UniRule annotation
    Disulfide bondi253 ↔ 296UniRule annotation

    Keywords - PTMi

    Disulfide bondUniRule annotation

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000165530.
    KOiK01176.
    OMAiNQTQVED.
    OrthoDBiEOG7RBZJ4.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013777. A-amylase_fun.
    IPR015340. A_amylase_DUF1966_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF09260. DUF1966. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001024. Alph-amyl_fung. 1 hit.
    PRINTSiPR00110. ALPHAAMYLASE.
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    G5EB45-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRSPLFLSFA ATVLAATPAE WRSQSIYFLL TDRFARTDNS TTAECDTSAK    50
    YCGGTWQGII NQLDYIQGMG FTAIWITPVT ANLEDGQHGE AYHGYWQQDI 100
    YALNPHFGTQ DDLRALSDAL HDRGMYLMVD VVANHFGYDA PAASVDYSAF 150
    NPFNSADYFH TPCDITDYDN QTQVEDCWLY TDAVSLPDVD TTNEEVKEIW 200
    YDWVGDLVSD YSIDGLRIDT ARHVQKDFWR DYNDAAGVYC VGEVFQGDPD 250
    YTCGYQEVMD GVLNYPIYYP LLRAFSSTSG SLSDLANMIE TVKYTCSDAT 300
    LLGNFIENHD NPRFASYTDD ISLAKNVAAF VILSDGIPII YAGQEQHYSG 350
    AGDPANREAT WLSGYDSTSE LYQFISKTNQ IRNHAIWQNE TYLSYKNYAI 400
    YNENNVLAMR KGFDGSQIIT ILTNAGADAG SSTVSVPNTG FTAGAAVTEI 450
    YTCEDITVSG SGEVSVPMES GLPRVLYPKA KLEGSGICGL 490
    Length:490
    Mass (Da):54,250
    Last modified:December 14, 2011 - v1
    Checksum:iA891C4ACEAEB5305
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BN001307 Genomic DNA. Translation: CBF86021.1.
    AACD01000032 Genomic DNA. Translation: EAA64850.1.
    RefSeqiXP_659622.1. XM_654530.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00008686; CADANIAP00008686; CADANIAG00008686.
    GeneIDi2875021.
    KEGGiani:AN2018.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BN001307 Genomic DNA. Translation: CBF86021.1 .
    AACD01000032 Genomic DNA. Translation: EAA64850.1 .
    RefSeqi XP_659622.1. XM_654530.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00008686 ; CADANIAP00008686 ; CADANIAG00008686 .
    GeneIDi 2875021.
    KEGGi ani:AN2018.2.

    Phylogenomic databases

    HOGENOMi HOG000165530.
    KOi K01176.
    OMAi NQTQVED.
    OrthoDBi EOG7RBZJ4.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR013777. A-amylase_fun.
    IPR015340. A_amylase_DUF1966_C.
    IPR006046. Alpha_amylase.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF09260. DUF1966. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001024. Alph-amyl_fung. 1 hit.
    PRINTSi PR00110. ALPHAAMYLASE.
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139Imported.
    2. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139Imported.
    3. "The 2008 update of the Aspergillus nidulans genome annotation: A community effort."
      Russo Wortman J., Mabey Gilsenan J., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J.M., Durek P., Espeso E., Fekete E., Flipphi M., Garcia Estrada C.
      , Geysens S., Goldman G., de Groot P.W.J., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A.K.W., Kim J-M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., MacCabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Posci I., Punt P.J., Ram A.F.J., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van der Vondervoot P.J.I., de Vries R.P., Walton J., Xiang X., Xiong Y., Ping Zeng A., Brandt B.W., Cornell M.J., van den Hondel C.A.M.J.J., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-S13(2009)
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: FGSC A4Imported.

    Entry informationi

    Entry nameiG5EB45_EMENI
    AccessioniPrimary (citable) accession number: G5EB45
    Secondary accession number(s): C8VLE6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: December 14, 2011
    Last sequence update: December 14, 2011
    Last modified: October 1, 2014
    This is version 21 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3