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Protein

Plasma membrane calcium-transporting ATPase 1

Gene

Atp2b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Involved in bone homeostasis. Has a role in osteoclastogenesis where it regulates RANKL-induced calcium oscillation, a key step in the differentiation process. Also promotes survival of mature osteoclasts, probably by preventing toxic accumulation of intracellular calcium.UniRule annotation1 Publication

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei475 – 47514-aspartylphosphate intermediateBy similarity
Metal bindingi797 – 7971MagnesiumBy similarity
Metal bindingi801 – 8011MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-418359. Reduction of cytosolic Ca++ levels.
R-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane calcium-transporting ATPase 11 Publication (EC:3.6.3.8UniRule annotation)
Short name:
PMCA11 Publication
Alternative name(s):
Plasma membrane calcium ATPase isoform 11 Publication
Plasma membrane calcium pump isoform 1Curated
Gene namesi
Name:Atp2b1Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:104653. Atp2b1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 108107CytoplasmicSequence analysisAdd
BLAST
Transmembranei109 – 12921HelicalSequence analysisAdd
BLAST
Topological domaini130 – 15122ExtracellularSequence analysisAdd
BLAST
Transmembranei152 – 17221HelicalSequence analysisAdd
BLAST
Topological domaini173 – 378206CytoplasmicSequence analysisAdd
BLAST
Transmembranei379 – 39921HelicalSequence analysisAdd
BLAST
Topological domaini400 – 41819ExtracellularSequence analysisAdd
BLAST
Transmembranei419 – 43921HelicalSequence analysisAdd
BLAST
Topological domaini440 – 855416CytoplasmicSequence analysisAdd
BLAST
Transmembranei856 – 87621HelicalSequence analysisAdd
BLAST
Topological domaini877 – 8837ExtracellularSequence analysis
Transmembranei884 – 90421HelicalSequence analysisAdd
BLAST
Topological domaini905 – 93026CytoplasmicSequence analysisAdd
BLAST
Transmembranei931 – 95121HelicalSequence analysisAdd
BLAST
Topological domaini952 – 96918ExtracellularSequence analysisAdd
BLAST
Transmembranei970 – 98920HelicalSequence analysisAdd
BLAST
Topological domaini990 – 100617CytoplasmicSequence analysisAdd
BLAST
Transmembranei1007 – 102721HelicalSequence analysisAdd
BLAST
Topological domaini1028 – 104013ExtracellularSequence analysisAdd
BLAST
Transmembranei1041 – 106121HelicalSequence analysisAdd
BLAST
Topological domaini1062 – 1220159CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Complete embryonic lethality.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 12201219Plasma membrane calcium-transporting ATPase 1CuratedPRO_0000430669Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycineBy similarity
Modified residuei8 – 81PhosphoserineBy similarity
Modified residuei17 – 171PhosphoserineCombined sources
Modified residuei338 – 3381PhosphoserineCombined sources
Modified residuei1116 – 11161Phosphothreonine; by PKCBy similarity
Modified residuei1140 – 11401PhosphoserineBy similarity
Modified residuei1155 – 11551PhosphoserineCombined sources
Modified residuei1165 – 11651PhosphothreonineCombined sources
Modified residuei1178 – 11781PhosphoserineCombined sources
Modified residuei1182 – 11821PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiG5E829.
PaxDbiG5E829.
PeptideAtlasiG5E829.
PRIDEiG5E829.

PTM databases

iPTMnetiG5E829.
PhosphoSiteiG5E829.

Expressioni

Tissue specificityi

Expressed in the retina, with strongest expression in the outer plexiform layer and lower expression levels in the inner nuclear layer and the inner plexiform layer (PubMed:12209837). Specifically expressed in the following retinal cell types: photoreceptor cells, cone bipolar cells and horizontal cells (PubMed:12209837). Expressed in osteoclasts (at protein level) (PubMed:23266958). Expressed at highest levels in brain, intestine, kidney, and stomach, and at lower levels in liver, lung, aorta, portal vein, urinary bladder, diaphragm, seminal vesicles and testes (PubMed:15178683).3 Publications

Developmental stagei

Up-regulated in differentiating osteoclasts.1 Publication

Gene expression databases

BgeeiENSMUSG00000019943.
GenevisibleiG5E829. MM.

Interactioni

Subunit structurei

Interacts with PDZD11. Interacts with SLC35G1 and STIM1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212573. 5 interactions.
IntActiG5E829. 3 interactions.
STRINGi10090.ENSMUSP00000020107.

Structurei

3D structure databases

ProteinModelPortaliG5E829.
SMRiG5E829. Positions 66-946, 1100-1127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1100 – 111718Calmodulin-binding subdomain ABy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
InParanoidiG5E829.
KOiK05850.
OMAiNNALCGE.
OrthoDBiEOG091G057D.
TreeFamiTF300330.

Family and domain databases

Gene3Di1.20.1110.10. 1 hit.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030319. ATP2B1/4.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF276. PTHR24093:SF276. 3 hits.
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G5E829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDMANNSVA YSGVKNSLKE ANHDGDFGIT LTELRALMEL RSTDALRKIQ
60 70 80 90 100
ESYGDVYGIC TKLKTSPNEG LSGNPADLER REAVFGKNFI PPKKPKTFLQ
110 120 130 140 150
LVWEALQDVT LIILEIAAIV SLGLSFYQPP EGDNALCGEV SVGEEEGEGE
160 170 180 190 200
TGWIEGAAIL LSVVCVVLVT AFNDWSKEKQ FRGLQSRIEQ EQKFTVIRGG
210 220 230 240 250
QVIQIPVADI TVGDIAQVKY GDLLPADGIL IQGNDLKIDE SSLTGESDHV
260 270 280 290 300
KKSLDKDPLL LSGTHVMEGS GRMVVTAVGV NSQTGIIFTL LGAGGEEEEK
310 320 330 340 350
KDEKKKEKKN KKQDGAIENR NKAKAQDGAA MEMQPLKSEE GGDGDEKDKK
360 370 380 390 400
KANLPKKEKS VLQGKLTKLA VQIGKAGLLM SAITVIILVL YFVIDTFWVQ
410 420 430 440 450
KRPWLAECTP IYIQYFVKFF IIGVTVLVVA VPEGLPLAVT ISLAYSVKKM
460 470 480 490 500
MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA YINEKHYKKV
510 520 530 540 550
PEPEAIPPNI LSYLVTGISV NCAYTSKILP PEKEGGLPRH VGNKTECALL
560 570 580 590 600
GFLLDLKRDY QDVRNEIPEE ALYKVYTFNS VRKSMSTVLK NSDGSFRIFS
610 620 630 640 650
KGASEIILKK CFKILSANGE AKVFRPRDRD DIVKTVIEPM ASEGLRTICL
660 670 680 690 700
AFRDFPAGEP EPEWDNENDV VTGLTCIAVV GIEDPVRPEV PEAIKKCQRA
710 720 730 740 750
GITVRMVTGD NINTARAIAT KCGILHPGED FLCLEGKDFN RRIRNEKGEI
760 770 780 790 800
EQERIDKIWP KLRVLARSSP TDKHTLVKGI IDSTVSEQRQ VVAVTGDGTN
810 820 830 840 850
DGPALKKADV GFAMGIAGTD VAKEASDIIL TDDNFTSIVK AVMWGRNVYD
860 870 880 890 900
SISKFLQFQL TVNVVAVIVA FTGACITQDS PLKAVQMLWV NLIMDTLASL
910 920 930 940 950
ALATEPPTES LLLRKPYGRN KPLISRTMMK NILGHAFYQL VVVFTLLFAG
960 970 980 990 1000
EKFFDIDSGR NAPLHAPPSE HYTIVFNTFV LMQLFNEINA RKIHGERNVF
1010 1020 1030 1040 1050
EGIFNNAIFC TIVLGTFVVQ IIIVQFGGKP FSCSELSIEQ WLWSIFLGMG
1060 1070 1080 1090 1100
TLLWGQLIST IPTSRLKFLK EAGHGTQKEE IPEEELAEDV EEIDHAEREL
1110 1120 1130 1140 1150
RRGQILWFRG LNRIQTQIRV VNAFRSSLYE GLEKPESRSS IHNFMTHPEF
1160 1170 1180 1190 1200
RIEDSEPHIP LIDDTDAEDD APTKRNSSPP PSPNKNNNAV DSGIHLTIEM
1210 1220
NKSATSSSPG SPLHSLETSL
Length:1,220
Mass (Da):134,747
Last modified:December 14, 2011 - v1
Checksum:i6B3DDAA2DBB001C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC153906 Genomic DNA. No translation available.
AC160410 Genomic DNA. No translation available.
CH466539 Genomic DNA. Translation: EDL21639.1.
CCDSiCCDS24145.1.
RefSeqiNP_080758.1. NM_026482.2.
XP_011241851.1. XM_011243549.1.
UniGeneiMm.166944.
Mm.471912.

Genome annotation databases

EnsembliENSMUST00000020107; ENSMUSP00000020107; ENSMUSG00000019943.
GeneIDi67972.
KEGGimmu:67972.
UCSCiuc007gxf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC153906 Genomic DNA. No translation available.
AC160410 Genomic DNA. No translation available.
CH466539 Genomic DNA. Translation: EDL21639.1.
CCDSiCCDS24145.1.
RefSeqiNP_080758.1. NM_026482.2.
XP_011241851.1. XM_011243549.1.
UniGeneiMm.166944.
Mm.471912.

3D structure databases

ProteinModelPortaliG5E829.
SMRiG5E829. Positions 66-946, 1100-1127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212573. 5 interactions.
IntActiG5E829. 3 interactions.
STRINGi10090.ENSMUSP00000020107.

PTM databases

iPTMnetiG5E829.
PhosphoSiteiG5E829.

Proteomic databases

MaxQBiG5E829.
PaxDbiG5E829.
PeptideAtlasiG5E829.
PRIDEiG5E829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020107; ENSMUSP00000020107; ENSMUSG00000019943.
GeneIDi67972.
KEGGimmu:67972.
UCSCiuc007gxf.2. mouse.

Organism-specific databases

CTDi490.
MGIiMGI:104653. Atp2b1.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
GeneTreeiENSGT00510000046331.
InParanoidiG5E829.
KOiK05850.
OMAiNNALCGE.
OrthoDBiEOG091G057D.
TreeFamiTF300330.

Enzyme and pathway databases

ReactomeiR-MMU-418359. Reduction of cytosolic Ca++ levels.
R-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Miscellaneous databases

ChiTaRSiAtp2b1. mouse.
PROiG5E829.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019943.
GenevisibleiG5E829. MM.

Family and domain databases

Gene3Di1.20.1110.10. 1 hit.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR030319. ATP2B1/4.
IPR022141. ATP_Ca_trans_C.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PANTHERiPTHR24093:SF276. PTHR24093:SF276. 3 hits.
PfamiPF12424. ATP_Ca_trans_C. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 2 hits.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT2B1_MOUSE
AccessioniPrimary (citable) accession number: G5E829
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: December 14, 2011
Last modified: September 7, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.