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G5AY81 (HYAS2_HETGA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hyaluronan synthase 2

EC=2.4.1.212
Alternative name(s):
Hyaluronate synthase 2
Hyaluronic acid synthase 2
Gene names
Name:Has2
OrganismHeterocephalus glaber (Naked mole rat) [Complete proteome]
Taxonomic identifier10181 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiBathyergidaeHeterocephalus

Protein attributes

Sequence length552 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix. Ref.2

Catalytic activity

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).

UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).

Cofactor

Magnesium.

Pathway

Glycan biosynthesis; hyaluronan biosynthesis.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Miscellaneous

Via the strong production of high molecular mass hyaluronan (HMM-HA) may be in part responsible for the low susceptibility to cancers observed with those rodents (Ref.2).

Sequence similarities

Belongs to the NodC/HAS family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 552552Hyaluronan synthase 2
PRO_0000424266

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221Helical; Name=1; Potential
Topological domain33 – 4513Extracellular Potential
Transmembrane46 – 6621Helical; Name=2; Potential
Topological domain67 – 374308Cytoplasmic Potential
Transmembrane375 – 39521Helical; Name=3; Potential
Topological domain396 – 4027Extracellular Potential
Transmembrane403 – 42321Helical; Name=4; Potential
Topological domain424 – 4296Cytoplasmic Potential
Transmembrane430 – 45021Helical; Name=5; Potential
Topological domain451 – 47525Extracellular Potential
Transmembrane476 – 49621Helical; Name=6; Potential
Topological domain497 – 51014Cytoplasmic Potential
Transmembrane511 – 53121Helical; Name=7; Potential
Topological domain532 – 55221Extracellular Potential

Sequences

Sequence LengthMass (Da)Tools
G5AY81 [UniParc].

Last modified December 14, 2011. Version 1.
Checksum: 36C8A97AD5E3729E

FASTA55263,442
        10         20         30         40         50         60 
MHCERFLCIL RIIGTTLFGV SLLLGITAAY IVGYQFIQTD NYYFSFGLYG AFLASHLIIQ 

        70         80         90        100        110        120 
SLFAFLEHRK MKKSLETPIK LNKTVALCIA AYQEDPDYLR KCLQSVKRLT YPGIKVVMVI 

       130        140        150        160        170        180 
DGNSDDDLYM MDIFSEVMGR DKSATYIWKN NFHEKGPGET DESHKESSQH VTQLVLSSKS 

       190        200        210        220        230        240 
VCIMQKWGGK REVMYTAFRA LGRSVDYVQV CDSDTMLDPA SSVEMVKVLE EDPMVGGVGG 

       250        260        270        280        290        300 
DVQILNKYDS WISFLSSVRY WMAFNIERAC QSYFGCVQCI SGPLGMYRNS LLHEFVEDWY 

       310        320        330        340        350        360 
SQEFMGNQCS FGDDRHLTNR VLSLGYATKY TARSKCLTET PIEYLRWLNQ QTRWSKSYFR 

       370        380        390        400        410        420 
EWLYNAMWFH KHHLWMTYEA VITGFFPFFL IATVIQLFYR GKIWNILLFL LTVQLVGLIK 

       430        440        450        460        470        480 
SSFASCLRGN IVMVFMSLYS VLYMSSLLPA KMFAIATINK AGWGTSGRKT IVVNFIGLIP 

       490        500        510        520        530        540 
VSVWFTILLG GVIFTIYKES KKPFSESKQT VLIVGTLLYA CYWVMLLTLY VVLINKCGRR 

       550 
KKGQQYDMVL DV 

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Web resources

Protein Spotlight

an unexpected turn of events - Issue 155 of December 2013

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
JH167500 Genomic DNA. Translation: EHB01992.1.
RefSeqXP_004883180.1. XM_004883123.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101724130.
KEGGhgl:101724130.

Organism-specific databases

CTD3037.

Phylogenomic databases

KOK00752.

Enzyme and pathway databases

UniPathwayUPA00341.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR001173. Glyco_trans_2-like.
IPR026107. HAS/NodC.
IPR028371. HAS2.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF7. PTHR22913:SF7. 1 hit.
PfamPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 2 hits.
ProtoNetSearch...

Entry information

Entry nameHYAS2_HETGA
AccessionPrimary (citable) accession number: G5AY81
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: December 14, 2011
Last modified: June 11, 2014
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PATHWAY comments

Index of metabolic and biosynthesis pathways