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G5AY81

- HYAS2_HETGA

UniProt

G5AY81 - HYAS2_HETGA

Protein

Hyaluronan synthase 2

Gene

Has2

Organism
Heterocephalus glaber (Naked mole rat)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 14 (01 Oct 2014)
      Sequence version 1 (14 Dec 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix.1 Publication

    Catalytic activityi

    UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).
    UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).

    Cofactori

    Magnesium.

    Pathwayi

    GO - Molecular functioni

    1. hyaluronan synthase activity Source: UniProtKB

    GO - Biological processi

    1. atrioventricular canal development Source: UniProtKB
    2. endocardial cushion to mesenchymal transition Source: UniProtKB
    3. extracellular matrix assembly Source: UniProtKB
    4. extracellular polysaccharide biosynthetic process Source: UniProtKB
    5. hyaluronan biosynthetic process Source: UniProtKB
    6. positive regulation of urine volume Source: UniProtKB
    7. renal water absorption Source: UniProtKB
    8. vasculogenesis Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00341.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronan synthase 2 (EC:2.4.1.212)
    Alternative name(s):
    Hyaluronate synthase 2
    Hyaluronic acid synthase 2
    Gene namesi
    Name:Has2
    OrganismiHeterocephalus glaber (Naked mole rat)
    Taxonomic identifieri10181 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiBathyergidaeHeterocephalus
    ProteomesiUP000006813: Unassembled WGS sequence

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: InterPro

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 552552Hyaluronan synthase 2PRO_0000424266Add
    BLAST

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 4513ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini67 – 374308CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini396 – 4027ExtracellularSequence Analysis
    Topological domaini424 – 4296CytoplasmicSequence Analysis
    Topological domaini451 – 47525ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini497 – 51014CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini532 – 55221ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei46 – 6621Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei375 – 39521Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei403 – 42321Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei430 – 45021Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei476 – 49621Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei511 – 53121Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NodC/HAS family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK00752.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR026107. HAS/NodC.
    IPR028371. HAS2.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR22913. PTHR22913. 1 hit.
    PTHR22913:SF7. PTHR22913:SF7. 1 hit.
    PfamiPF00535. Glycos_transf_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    G5AY81-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHCERFLCIL RIIGTTLFGV SLLLGITAAY IVGYQFIQTD NYYFSFGLYG    50
    AFLASHLIIQ SLFAFLEHRK MKKSLETPIK LNKTVALCIA AYQEDPDYLR 100
    KCLQSVKRLT YPGIKVVMVI DGNSDDDLYM MDIFSEVMGR DKSATYIWKN 150
    NFHEKGPGET DESHKESSQH VTQLVLSSKS VCIMQKWGGK REVMYTAFRA 200
    LGRSVDYVQV CDSDTMLDPA SSVEMVKVLE EDPMVGGVGG DVQILNKYDS 250
    WISFLSSVRY WMAFNIERAC QSYFGCVQCI SGPLGMYRNS LLHEFVEDWY 300
    SQEFMGNQCS FGDDRHLTNR VLSLGYATKY TARSKCLTET PIEYLRWLNQ 350
    QTRWSKSYFR EWLYNAMWFH KHHLWMTYEA VITGFFPFFL IATVIQLFYR 400
    GKIWNILLFL LTVQLVGLIK SSFASCLRGN IVMVFMSLYS VLYMSSLLPA 450
    KMFAIATINK AGWGTSGRKT IVVNFIGLIP VSVWFTILLG GVIFTIYKES 500
    KKPFSESKQT VLIVGTLLYA CYWVMLLTLY VVLINKCGRR KKGQQYDMVL 550
    DV 552
    Length:552
    Mass (Da):63,442
    Last modified:December 14, 2011 - v1
    Checksum:i36C8A97AD5E3729E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JH167500 Genomic DNA. Translation: EHB01992.1.
    RefSeqiXP_004883180.1. XM_004883123.1.

    Genome annotation databases

    GeneIDi101724130.
    KEGGihgl:101724130.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    an unexpected turn of events - Issue 155 of December 2013

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    JH167500 Genomic DNA. Translation: EHB01992.1 .
    RefSeqi XP_004883180.1. XM_004883123.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101724130.
    KEGGi hgl:101724130.

    Organism-specific databases

    CTDi 3037.

    Phylogenomic databases

    KOi K00752.

    Enzyme and pathway databases

    UniPathwayi UPA00341 .

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR026107. HAS/NodC.
    IPR028371. HAS2.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR22913. PTHR22913. 1 hit.
    PTHR22913:SF7. PTHR22913:SF7. 1 hit.
    Pfami PF00535. Glycos_transf_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "High-molecular-mass hyaluronan mediates the cancer resistance of the naked mole rat."
      Tian X., Azpurua J., Hine C., Vaidya A., Myakishev-Rempel M., Ablaeva J., Mao Z., Nevo E., Gorbunova V., Seluanov A.
      Nature 499:346-349(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiHYAS2_HETGA
    AccessioniPrimary (citable) accession number: G5AY81
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2013
    Last sequence update: December 14, 2011
    Last modified: October 1, 2014
    This is version 14 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Via the strong production of high molecular mass hyaluronan (HMM-HA) may be in part responsible for the low susceptibility to cancers observed with those rodents.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3