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Protein

Hyaluronan synthase 2

Gene

Has2

Organism
Heterocephalus glaber (Naked mole rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix.1 Publication

Miscellaneous

Via the strong production of high molecular mass hyaluronan (HMM-HA) may be in part responsible for the low susceptibility to cancers observed with those rodents.1 Publication

Catalytic activityi

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).

Cofactori

Pathwayi: hyaluronan biosynthesis

This protein is involved in the pathway hyaluronan biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway hyaluronan biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00341.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronan synthase 2 (EC:2.4.1.212)
Alternative name(s):
Hyaluronate synthase 2
Hyaluronic acid synthase 2
Gene namesi
Name:Has2
OrganismiHeterocephalus glaber (Naked mole rat)
Taxonomic identifieri10181 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricomorphaBathyergidaeHeterocephalus
Proteomesi
  • UP000006813 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini33 – 45ExtracellularSequence analysisAdd BLAST13
Transmembranei46 – 66Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini67 – 374CytoplasmicSequence analysisAdd BLAST308
Transmembranei375 – 395Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini396 – 402ExtracellularSequence analysis7
Transmembranei403 – 423Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini424 – 429CytoplasmicSequence analysis6
Transmembranei430 – 450Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini451 – 475ExtracellularSequence analysisAdd BLAST25
Transmembranei476 – 496Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini497 – 510CytoplasmicSequence analysisAdd BLAST14
Transmembranei511 – 531Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini532 – 552ExtracellularSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004242661 – 552Hyaluronan synthase 2Add BLAST552

Family & Domainsi

Sequence similaritiesi

Belongs to the NodC/HAS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiG5AY81.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR001173. Glyco_trans_2-like.
IPR028371. HAS2.
IPR029044. Nucleotide-diphossugar_trans.
PANTHERiPTHR22913:SF17. PTHR22913:SF17. 1 hit.
PfamiView protein in Pfam
PF00535. Glycos_transf_2. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

G5AY81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHCERFLCIL RIIGTTLFGV SLLLGITAAY IVGYQFIQTD NYYFSFGLYG
60 70 80 90 100
AFLASHLIIQ SLFAFLEHRK MKKSLETPIK LNKTVALCIA AYQEDPDYLR
110 120 130 140 150
KCLQSVKRLT YPGIKVVMVI DGNSDDDLYM MDIFSEVMGR DKSATYIWKN
160 170 180 190 200
NFHEKGPGET DESHKESSQH VTQLVLSSKS VCIMQKWGGK REVMYTAFRA
210 220 230 240 250
LGRSVDYVQV CDSDTMLDPA SSVEMVKVLE EDPMVGGVGG DVQILNKYDS
260 270 280 290 300
WISFLSSVRY WMAFNIERAC QSYFGCVQCI SGPLGMYRNS LLHEFVEDWY
310 320 330 340 350
SQEFMGNQCS FGDDRHLTNR VLSLGYATKY TARSKCLTET PIEYLRWLNQ
360 370 380 390 400
QTRWSKSYFR EWLYNAMWFH KHHLWMTYEA VITGFFPFFL IATVIQLFYR
410 420 430 440 450
GKIWNILLFL LTVQLVGLIK SSFASCLRGN IVMVFMSLYS VLYMSSLLPA
460 470 480 490 500
KMFAIATINK AGWGTSGRKT IVVNFIGLIP VSVWFTILLG GVIFTIYKES
510 520 530 540 550
KKPFSESKQT VLIVGTLLYA CYWVMLLTLY VVLINKCGRR KKGQQYDMVL

DV
Length:552
Mass (Da):63,442
Last modified:December 14, 2011 - v1
Checksum:i36C8A97AD5E3729E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JH167500 Genomic DNA. Translation: EHB01992.1.

Similar proteinsi

Entry informationi

Entry nameiHYAS2_HETGA
AccessioniPrimary (citable) accession number: G5AY81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: December 14, 2011
Last modified: September 27, 2017
This is version 23 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families