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G4SUF2 (G4SUF2_META2) Unreviewed, UniProtKB/TrEMBL

Last modified February 6, 2013. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Imidazole glycerol phosphate synthase subunit HisH HAMAP-Rule MF_00278
EC=2.4.2.- HAMAP-Rule MF_00278
Alternative name(s):
IGP synthase glutamine amidotransferase subunit HAMAP-Rule MF_00278
IGP synthase subunit HisH HAMAP-Rule MF_00278
ImGP synthase subunit HisH HAMAP-Rule MF_00278
Gene names
Name:hisH HAMAP-Rule MF_00278
Ordered Locus Names:MEALZ_0074 EMBL CCE21778.1
OrganismMethylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z) [Complete proteome] [HAMAP]
Taxonomic identifier1091494 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Methylococcales › Methylococcaceae › Methylomicrobium ›

Protein attributes

Sequence length216 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR By similarity. SAAS SAAS010139 HAMAP-Rule MF_00278

Catalytic activity

5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O. SAAS SAAS010139 HAMAP-Rule MF_00278

Pathway

Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. SAAS SAAS010139 HAMAP-Rule MF_00278

Subunit structure

Heterodimer of HisH and HisF By similarity. SAAS SAAS010139 HAMAP-Rule MF_00278

Subcellular location

Cytoplasm By similarity SAAS SAAS010139 HAMAP-Rule MF_00278.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain. SAAS SAAS010139 HAMAP-Rule MF_00278

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain3 – 216214Glutamine amidotransferase type-1 By similarity HAMAP-Rule MF_00278

Sites

Active site821Nucleophile By similarity HAMAP-Rule MF_00278
Active site1911 By similarity HAMAP-Rule MF_00278
Active site1931 By similarity HAMAP-Rule MF_00278

Sequences

Sequence LengthMass (Da)Tools
G4SUF2 [UniParc].

Last modified December 14, 2011. Version 1.
Checksum: C7A93D0DF5A228D5

FASTA21623,727
        10         20         30         40         50         60 
MSSVAVIDYG MGNLHSIAKA LQHADPRTEV RVVSDIESVL RADRVVFPGV GAIGDCMQAL 

        70         80         90        100        110        120 
NASGLASAIK TAAQEKPMLG ICLGMQALLT DSEESDATMC LNIIPGHVQR FSDNLQDKNG 

       130        140        150        160        170        180 
ERLKIPHMGW NRVKQQPHPL WQNIEPGSRF YFVHSYYAVP EDESVVSGTS DYPKPFACAL 

       190        200        210 
AKANIFAVQF HPEKSQSAGL QLLKNFLQWD GQFNVN

« Hide

References

[1]"Genome sequence of the haloalkaliphilic methanotrophic bacterium Methylomicrobium alcaliphilum 20Z."
Vuilleumier S., Khmelenina V.N., Bringel F., Reshetnikov A.S., Lajus A., Mangenot S., Rouy Z., Op den Camp H.J., Jetten M.S., Dispirito A.A., Dunfield P., Klotz M.G., Semrau J.D., Stein L.Y., Barbe V., Medigue C., Trotsenko Y.A., Kalyuzhnaya M.G.
J. Bacteriol. 194:551-552(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FO082060 Genomic DNA. Translation: CCE21778.1.
RefSeqYP_004915386.1. NC_016112.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID11362327.
KEGGmah:MEALZ_0074.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK02501.

Enzyme and pathway databases

BioCycMALC271065:GJFB-74-MONOMER.
UniPathwayUPA00031; UER00010.

Family and domain databases

HAMAPMF_00278. HisH.
InterProIPR017926. GATASE_1.
IPR010139. Imidazole-glycPsynth_HisH.
[Graphical view]
PfamPF00117. GATase. 1 hit.
[Graphical view]
PIRSFPIRSF000495. Amidotransf_hisH. 1 hit.
TIGRFAMsTIGR01855. IMP_synth_hisH. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG4SUF2_META2
AccessionPrimary (citable) accession number: G4SUF2
Entry history
Integrated into UniProtKB/TrEMBL: December 14, 2011
Last sequence update: December 14, 2011
Last modified: February 6, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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