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G4RGJ8 (G4RGJ8_PELHB) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607

EC=2.1.1.182 HAMAP-Rule MF_00607
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase HAMAP-Rule MF_00607
16S rRNA dimethyladenosine transferase HAMAP-Rule MF_00607
16S rRNA dimethylase HAMAP-Rule MF_00607
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase HAMAP-Rule MF_00607
Gene names
Name:rsmA HAMAP-Rule MF_00607
Synonyms:ksgA HAMAP-Rule MF_00607
Ordered Locus Names:KKY_1022 EMBL AEQ51057.1
OrganismPelagibacterium halotolerans (strain JCM 15775 / CGMCC 1.7692 / B2) [Complete proteome] [HAMAP] EMBL AEQ51057.1
Taxonomic identifier1082931 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesHyphomicrobiaceaePelagibacterium

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607 SAAS SAAS020596

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607 SAAS SAAS020596

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00607 SAAS SAAS023165.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily. HAMAP-Rule MF_00607

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Binding site281S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_00607
Binding site301S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP-Rule MF_00607
Binding site551S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_00607
Binding site771S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607
Binding site1031S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607
Binding site1221S-adenosyl-L-methionine By similarity HAMAP-Rule MF_00607

Sequences

Sequence LengthMass (Da)Tools
G4RGJ8 [UniParc].

Last modified December 14, 2011. Version 1.
Checksum: 9C2523EC8227D2A0

FASTA28130,722
        10         20         30         40         50         60 
MSQIDSLPPL REVIAEHGLR AKKELGQNFL LDLNLTARIA RSAGDLTDVT VIEVGPGPGG 

        70         80         90        100        110        120 
LTRALLAAGA KKVIAIERDE RALPALAQIS QAYRGRLEVI SADALEIDYR VLAEGRTKIV 

       130        140        150        160        170        180 
ANLPYNIATP LLTGWLSQEP WPAWFESLTL MFQREVAERI VARPGDKAYG RLGVLTGWRG 

       190        200        210        220        230        240 
DARIAMNLDR NAFTPPPKVT SSVVHIVPKQ VETDVPVRAL EEVTRHAFGQ RRKMIRQSLK 

       250        260        270        280 
GLGVPLEALF GAVGLKGDER AETLPVSTYI AMAREVAKLR G 

« Hide

References

[1]"Complete Genome Sequence of Pelagibacterium halotolerans B2T."
Huo Y.Y., Cheng H., Han X.F., Jiang X.W., Sun C., Zhang X.Q., Zhu X.F., Liu Y.F., Li P.F., Ni P.X., Wu M.
J. Bacteriol. 194:197-198(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 15775 / CGMCC 1.7692 / B2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003075 Genomic DNA. Translation: AEQ51057.1.
RefSeqYP_004898807.1. NC_016078.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEQ51057; AEQ51057; KKY_1022.
GeneID11345242.
KEGGphl:KKY_1022.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK02528.
OMAKMLRQSV.

Enzyme and pathway databases

BioCycPHAL1082931:GJXT-1019-MONOMER.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00755. ksgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG4RGJ8_PELHB
AccessionPrimary (citable) accession number: G4RGJ8
Entry history
Integrated into UniProtKB/TrEMBL: December 14, 2011
Last sequence update: December 14, 2011
Last modified: June 11, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)