Skip Header

Contribute Send feedback
Read comments (?) or add your own

G4RFG9 (G4RFG9_PELHB) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:KKY_1997 EMBL AEQ52007.1
OrganismPelagibacterium halotolerans (strain JCM 15775 / CGMCC 1.7692 / B2) [Complete proteome] [HAMAP]
Taxonomic identifier1082931 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Hyphomicrobiaceae › Pelagibacterium ›

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047 SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain101 – 300200ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding128 – 18255ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2501Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2671Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2671Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2691Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G4RFG9 [UniParc].

Last modified December 14, 2011. Version 1.
Checksum: ABD3E18802B00532

FASTA30633,319
        10         20         30         40         50         60 
MAKHVAVLKG GWSHEREVSL SSGGECAQAL RNAGYEVTEI DVKRDIASVL GELKPDVVFN 

        70         80         90        100        110        120 
ALHGRFGEDG TIQGLLEILG IPYTHSGVLA SSVAMDKHQA KVMFKSAGVP VTDHVIATRD 

       130        140        150        160        170        180 
AIARSHVMAP PYVVKPYNDG SSVNVFIVKE GQEHPPQEIL RTDWDGDEEL MVERFIPGRE 

       190        200        210        220        230        240 
LTCAVMGDVA LGVIEIVTDL AFYNYEAKYV SGGSRHELPA QVSPKIYEKV QKMSLMAHAA 

       250        260        270        280        290        300 
LGCRGVSRSD FRYDPRGGDD GELVCLEINT QPGMTKTSLV PELAAHAGHS FEELVSWMVE 


DASCNR

« Hide

References

[1]"Complete genome sequence of Pelagibacterium halotolerans B2T."
Huo Y.Y., Cheng H., Han X.F., Jiang X.W., Sun C., Zhang X.Q., Zhu X.F., Liu Y.F., Li P.F., Ni P.X., Wu M.
J. Bacteriol. 194:197-198(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 15775 / CGMCC 1.7692 / B2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003075 Genomic DNA. Translation: AEQ52007.1.
RefSeqYP_004899757.1. NC_016078.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEQ52007; AEQ52007; KKY_1997.
GeneID11343040.
KEGGphl:KKY_1997.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycPHAL1082931:GJXT-1992-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG4RFG9_PELHB
AccessionPrimary (citable) accession number: G4RFG9
Entry history
Integrated into UniProtKB/TrEMBL: December 14, 2011
Last sequence update: December 14, 2011
Last modified: April 3, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info