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G4REV1

- G4REV1_PELHB

UniProt

G4REV1 - G4REV1_PELHB

Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Pelagibacterium halotolerans (strain JCM 15775 / CGMCC 1.7692 / B2)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 20 (01 Oct 2014)
      Sequence version 1 (14 Dec 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

    Catalytic activityi

    ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei309 – 3091Coenzyme AUniRule annotation
    Binding sitei333 – 3331Coenzyme AUniRule annotation
    Binding sitei385 – 3851Substrate; via nitrogen amideUniRule annotation
    Binding sitei500 – 5001SubstrateUniRule annotation
    Binding sitei515 – 5151SubstrateUniRule annotation
    Active sitei517 – 5171UniRule annotation
    Binding sitei523 – 5231Coenzyme AUniRule annotation
    Binding sitei526 – 5261SubstrateUniRule annotation
    Metal bindingi537 – 5371Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi539 – 5391Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi542 – 5421Magnesium; via carbonyl oxygenUniRule annotation
    Binding sitei584 – 5841Coenzyme AUniRule annotation

    GO - Molecular functioni

    1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
    2. AMP binding Source: InterPro
    3. ATP binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from acetate Source: InterPro

    Keywords - Molecular functioni

    LigaseUniRule annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPHAL1082931:GJXT-1906-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
    Short name:
    AcCoA synthetaseUniRule annotation
    Short name:
    AcsUniRule annotation
    Alternative name(s):
    Acetate--CoA ligaseUniRule annotation
    Acyl-activating enzymeUniRule annotation
    Gene namesi
    Name:acsAUniRule annotation
    Ordered Locus Names:KKY_1911Imported
    OrganismiPelagibacterium halotolerans (strain JCM 15775 / CGMCC 1.7692 / B2)Imported
    Taxonomic identifieri1082931 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesHyphomicrobiaceaePelagibacterium
    ProteomesiUP000008850: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei609 – 6091N6-acetyllysineUniRule annotation

    Post-translational modificationi

    Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

    Keywords - PTMi

    AcetylationUniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliG4REV1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni409 – 4146Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

    Phylogenomic databases

    KOiK01895.
    OMAiQTAILFE.

    Family and domain databases

    HAMAPiMF_01123. Ac_CoA_synth.
    InterProiIPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    G4REV1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSEVFAPSD EVVAKTRVTA EQYDRMYRRS VSDPDGFWGE QAQRIDWIKP    50
    FTKVKNTSFE WPDVSIKWFE DGQLNVAANC IDRHLKEHGD TVAIIFEPDD 100
    PEAETRHITY RLLHGEVCRF ANVLKELGVQ KGERVTIYMP MIPEAAYAML 150
    ACARIGAVHS VVFGGFSPDA LAGRVNDCDS RVVITADEGC RGGKKVPLKV 200
    NVDKALEDCP GVEKVLVVRN TGSDIAMTSG RDVWLHEAEA GVEAVCEPEP 250
    MNAEDPLFIL YTSGSTGKPK GVLHTTGGYL VYASLTHELT FDYRQGEVFW 300
    CTADVGWVTG HSYIVYGPLA NGATTLMFEG VPNYPDAGRF WEVVDKHKVN 350
    IFYTAPTAIR ALMGAGNQFV ERADLSSLRL LGSVGEPINP EAWMWYHKQV 400
    GRERCIVVDT WWQTETGGFM ITPLPGAIPT KPGSATKPFF GVQPVVLEPE 450
    SGKVIEETEA AGVLAIADSW PGQMRTVYGD HQRFKETYFQ QYKGYYFTGD 500
    GCRRDEDGYY WITGRVDDVL NVSGHRLGTA EVESALVAHP KVSEAAVVGF 550
    PHDVKGQGIY CYVTLMAGEA ASDDLAGELR NWVRKEIGPI ASPDHIQFAP 600
    GLPKTRSGKI MRRILRKVAE NDYGALGDTS TLADPGVVDD LIANRKNR 648
    Length:648
    Mass (Da):71,561
    Last modified:December 14, 2011 - v1
    Checksum:i1940E68026A1A373
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003075 Genomic DNA. Translation: AEQ51922.1.
    RefSeqiWP_014131071.1. NC_016078.1.
    YP_004899672.1. NC_016078.1.

    Genome annotation databases

    EnsemblBacteriaiAEQ51922; AEQ51922; KKY_1911.
    GeneIDi11342874.
    KEGGiphl:KKY_1911.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003075 Genomic DNA. Translation: AEQ51922.1 .
    RefSeqi WP_014131071.1. NC_016078.1.
    YP_004899672.1. NC_016078.1.

    3D structure databases

    ProteinModelPortali G4REV1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEQ51922 ; AEQ51922 ; KKY_1911 .
    GeneIDi 11342874.
    KEGGi phl:KKY_1911.

    Phylogenomic databases

    KOi K01895.
    OMAi QTAILFE.

    Enzyme and pathway databases

    BioCyci PHAL1082931:GJXT-1906-MONOMER.

    Family and domain databases

    HAMAPi MF_01123. Ac_CoA_synth.
    InterProi IPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 15775 / CGMCC 1.7692 / B2Imported.

    Entry informationi

    Entry nameiG4REV1_PELHB
    AccessioniPrimary (citable) accession number: G4REV1
    Entry historyi
    Integrated into UniProtKB/TrEMBL: December 14, 2011
    Last sequence update: December 14, 2011
    Last modified: October 1, 2014
    This is version 20 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3