Phenylalanine--tRNA ligase beta subunit - Pelagibacterium halotolerans (strain JCM 15775 / CGMCC 1.7692 / B2)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta subunit HAMAP-Rule MF_00283
EC=6.1.1.20 HAMAP-Rule MF_00283

Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit HAMAP-Rule MF_00283

Gene names

Name:	pheT HAMAP-Rule MF_00283
Ordered Locus Names:	KKY_3713 EMBL AEQ53695.1

Organism

Pelagibacterium halotolerans (strain JCM 15775 / CGMCC 1.7692 / B2) [Complete proteome] [HAMAP]

Taxonomic identifier

1082931 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Hyphomicrobiaceae › Pelagibacterium ›

Protein attributes

Sequence length	802 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00283 SAAS SAAS002547
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP-Rule MF_00283 SAAS SAAS002547
Subunit structure	Tetramer of two alpha and two beta subunits By similarity. HAMAP-Rule MF_00283 SAAS SAAS002547
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00283.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. HAMAP-Rule MF_00283 Contains 1 B5 domain. HAMAP-Rule MF_00283 SAAS SAAS002547 Contains 1 FDX-ACB domain. HAMAP-Rule MF_00283 SAAS SAAS002547 Contains 1 tRNA-binding domain. HAMAP-Rule MF_00283 SAAS SAAS002547

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00283 SAAS SAAS002547
Cellular component	Cytoplasm HAMAP-Rule MF_00283 SAAS SAAS002547
Ligand	ATP-binding HAMAP-Rule MF_00283 SAAS SAAS002547 Magnesium SAAS SAAS002547 HAMAP-Rule MF_00283 Metal-binding HAMAP-Rule MF_00283 SAAS SAAS002547 Nucleotide-binding RNA-binding tRNA-binding HAMAP-Rule MF_00283 SAAS SAAS002547
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00283 SAAS SAAS002547 EMBL AEQ53695.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Domain

39 – 149

111

tRNA-binding By similarity HAMAP-Rule MF_00283

Domain

399 – 474

76

B5 By similarity HAMAP-Rule MF_00283

Domain

708 – 801

94

FDX-ACB By similarity HAMAP-Rule MF_00283

Sites

Metal binding

452

1

Magnesium By similarity HAMAP-Rule MF_00283

Metal binding

458

1

Magnesium; via carbonyl oxygen By similarity HAMAP-Rule MF_00283

Metal binding

461

1

Magnesium By similarity HAMAP-Rule MF_00283

Metal binding

462

1

Magnesium By similarity HAMAP-Rule MF_00283

Sequences

Sequence

Length

Mass (Da)

Tools

G4RBQ4 [UniParc].

Last modified December 14, 2011. Version 1.
Checksum: CA41450D9503C7BA
Show »

FASTA

802

84,812

        10         20         30         40         50         60 
MKFTLSWLKE HLETTATNAE IIDTLTMVGL EVEEVTDSGA ALKDFVTAHV VSAEQHPNAD 

        70         80         90        100        110        120 
KLRVCKVDAG TGELIDVVCG APNARTGMKS VFAFAGTYIP GKDITIGKGN IRGQVSNGML 

       130        140        150        160        170        180 
CSNAELELSD DHDGIIELPE DAPIGVPYPD YAGINEVVID VGLTPNRGDC AGVHGIARDL 

       190        200        210        220        230        240 
AAAGLGQLKD TSVEAVPATA GASPIAVRVD GDICRMFSGR LVRNVTNGPS PQWMQDRLRA 

       250        260        270        280        290        300 
VGLRPINALV DITNYVSLDR ARPLHVYDAD KLTGNIAARM GRTGESFAAL DGKTYAVDET 

       310        320        330        340        350        360 
MCVIADDQKV LGLGGIIGGE ETGSSDETTN VFIECAWFEP TAIAAAGRKT GINSDARYRF 

       370        380        390        400        410        420 
ERTVDPESVK PGIELATRLV MELCGGEPAE TVIAGHVGSP GTVIDFPFSE IERLTGLTVA 

       430        440        450        460        470        480 
ADEATAILTR LGFEVSGSGE VVRVGVPSWR PDVTMKADLV EEVMRIVGVD RVPVEPLPRL 

       490        500        510        520        530        540 
SGVAPKMLTP IQNRRRIARR ALAARGMDEA MTWSFISTDF AQAFGGGSPQ LRLANAIASD 

       550        560        570        580        590        600 
MTDMRPSLLP GLLSGAARNG NRGFDDVALF EVGQVFLSDA PEGQRNYATG IRTGTAGPSG 

       610        620        630        640        650        660 
AGRHWQGKAD KVGVYDAKAD LGAVLDALGV DIDKVQVVAE PAQWSHPGRG GRIQLGPKLI 

       670        680        690        700        710        720 
LGWFGELHPV MAEKFDLDGP VAAFEIDLDA LPEPRKKATK ARPPLALSAL QPVRRDFAFV 

       730        740        750        760        770        780 
VDTNVEAAGL LRAARGADKA LISDVTIFDV FAGAHVGEGN KSVAIEVTLQ PRDKTLTDEE 

       790        800 
IEAVAGKIVA AVEKATGGVL RG

« Hide

References

[1]

"Complete genome sequence of Pelagibacterium halotolerans B2T."
Huo Y.Y., Cheng H., Han X.F., Jiang X.W., Sun C., Zhang X.Q., Zhu X.F., Liu Y.F., Li P.F., Ni P.X., Wu M.
J. Bacteriol. 194:197-198(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 15775 / CGMCC 1.7692 / B2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003075 Genomic DNA. Translation: AEQ53695.1.
RefSeq	YP_004901445.1. NC_016078.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEQ53695; AEQ53695; KKY_3713.
GeneID	11342728.
KEGG	phl:KKY_3713.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01890.
Enzyme and pathway databases
BioCyc	PHAL1082931:GJXT-3705-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.30.56.20. 1 hit. 3.30.70.380. 1 hit. 3.50.40.10. 1 hit.
HAMAP	MF_00283. Phe_tRNA_synth_beta1.
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR004532. Phe-tRNA-ligase_IIc_bsu_bac. IPR020825. Phe-tRNA_synthase_B3/B4. IPR005121. PheS_beta_Fdx_antiC-bd. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF03147. FDX-ACB. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. SM00896. FDX-ACB. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit. SSF54991. Fdx_AntiC_bd. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. pheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G4RBQ4_PELHB

Accession

Primary (citable) accession number: G4RBQ4

Entry history

Integrated into UniProtKB/TrEMBL:	December 14, 2011
Last sequence update:	December 14, 2011
Last modified:	April 3, 2013
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information