G4RBB6 (G4RBB6_PELHB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120
Short name=Glu-ADT subunit A HAMAP-Rule MF_00120
EC=6.3.5.- HAMAP-Rule MF_00120

Gene names

Name:	gatA HAMAP-Rule MF_00120
Ordered Locus Names:	KKY_1597 EMBL AEQ51614.1

Organism

Pelagibacterium halotolerans (strain JCM 15775 / CGMCC 1.7692 / B2) [Complete proteome] [HAMAP]

Taxonomic identifier

1082931 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Hyphomicrobiaceae › Pelagibacterium › Pelagibacterium halotolerans

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. SAAS SAAS004412 HAMAP-Rule MF_00120
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00120
Sequence similarities	Belongs to the amidase family. GatA subfamily. HAMAP-Rule MF_00120

Ontologies

Keywords
Biological process	Protein biosynthesis SAAS SAAS004412 HAMAP-Rule MF_00120
Ligand	ATP-binding SAAS SAAS004412 HAMAP-Rule MF_00120 Nucleotide-binding
Molecular function	Ligase SAAS SAAS004412 HAMAP-Rule MF_00120 Transferase EMBL AEQ51614.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	79	1	Charge relay system By similarity HAMAP-Rule MF_00120
	Active site	159	1	Charge relay system By similarity HAMAP-Rule MF_00120
	Active site	183	1	Acyl-ester intermediate By similarity HAMAP-Rule MF_00120

Sequences

	Sequence		Length	Mass (Da)
	G4RBB6 [UniParc]. Last modified December 14, 2011. Version 1. Checksum: C71C5DB770386F0F	FASTA	494	52,526
	10 20 30 40 50 60 MTDLTRLSLK ALRDGIAAKE FTALEATDSY ISAIEAGNDK LNAYVAVTAD QAREMAKASD 70 80 90 100 110 120 AKIASGDNGM LEGVPLGIKD LFATKGVHTQ AASHILDGFK PEYESTVTEH LWADGAVMLG 130 140 150 160 170 180 KLNMDEFAMG SSNETSYYGP VVNPWRGEGG NKDLVPGGSS GGSAAAVSAW LCAGATATDT 190 200 210 220 230 240 GGSIRQPAAF TGTVGIKPTY GRCSRWGTVA FASSLDQAGP IARTVEDTAL MLQSMAGFDP 250 260 270 280 290 300 KDSTSVDMAV PDFATAVERG VKGMTIGVPA EYRMDGMPDE IEKLWSQGLE WLKAEGATVK 310 320 330 340 350 360 DISLPHTKYA LPAYYIVAPA EASSNLARYD GVKYGLRVSG KDITDMYELT RAEGFGREVK 370 380 390 400 410 420 RRVMIGTYVL SAGYYDAYYV RAQKVRTLIK RDFEDAFNAG VDAILTPATP SAAFGIADQE 430 440 450 460 470 480 LHADPVKMYL NDIFTVTVNM AGLPGIAVPA GKDGQGLPLG LQLIGKPFDE ETLFAAGRVI 490 EKSAALDLAP AKWW

References

[1]

"Complete genome sequence of Pelagibacterium halotolerans B2T." Huo Y.Y., Cheng H., Han X.F., Jiang X.W., Sun C., Zhang X.Q., Zhu X.F., Liu Y.F., Li P.F., Ni P.X., Wu M. J. Bacteriol. 194:197-198(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 15775 / CGMCC 1.7692 / B2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003075 Genomic DNA. Translation: AEQ51614.1.
RefSeq	YP_004899364.1. NC_016078.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEQ51614; AEQ51614; KKY_1597.
GeneID	11344509.
KEGG	phl:KKY_1597.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K02433.
Enzyme and pathway databases
BioCyc	PHAL1082931:GJXT-1592-MONOMER.
Family and domain databases
Gene3D	3.90.1300.10. 1 hit.
HAMAP	MF_00120. GatA.
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR004412. GatA. [Graphical view]
PANTHER	PTHR11895. PTHR11895. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMs	TIGR00132. gatA. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G4RBB6_PELHB

Accession

Primary (citable) accession number: G4RBB6

Entry history

Integrated into UniProtKB/TrEMBL:	December 14, 2011
Last sequence update:	December 14, 2011
Last modified:	April 3, 2013
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)