G4QYZ1 (G4QYZ1_CORPS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha subunit HAMAP-Rule MF_00281
EC=6.1.1.20 HAMAP-Rule MF_00281

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit HAMAP-Rule MF_00281

Gene names

Name:	pheS HAMAP-Rule MF_00281 EMBL AEP70196.1
ORF Names:	Cp4202_0938 EMBL AEP70196.1

Organism

Corynebacterium pseudotuberculosis 42/02-A EMBL AEP70196.1

Taxonomic identifier

1087453 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium pseudotuberculosis

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00281 SAAS SAAS004529
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP-Rule MF_00281 SAAS SAAS004529
Subunit structure	Tetramer of two alpha and two beta subunits By similarity. HAMAP-Rule MF_00281 SAAS SAAS004529
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00281 SAAS SAAS004529.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily. HAMAP-Rule MF_00281

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00281 SAAS SAAS004529
Cellular component	Cytoplasm HAMAP-Rule MF_00281 SAAS SAAS004529
Ligand	ATP-binding HAMAP-Rule MF_00281 SAAS SAAS004529 Magnesium HAMAP-Rule MF_00281 SAAS SAAS004529 Metal-binding HAMAP-Rule MF_00281 SAAS SAAS004529 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00281 SAAS SAAS004529 EMBL AEP70196.1 Ligase
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	269	1	Magnesium By similarity HAMAP-Rule MF_00281

Sequences

	Sequence		Length	Mass (Da)
	G4QYZ1 [UniParc]. Last modified December 14, 2011. Version 1. Checksum: 4DFBC0F75D7EB7B2	FASTA	351	38,745
	10 20 30 40 50 60 MSDQPDNSSV DLTEEGLNVA AEAAIEAFTT AQTLEDLAEA RRAHLGDSAP IPQARRALGS 70 80 90 100 110 120 IAKEKRKEAG RLVNMARGRV EKRYSEAKVV LEEKRNAEVL KAERVDVTVP TTRRQLGALH 130 140 150 160 170 180 PITELSETIA DIFVGMGYEV AEGPEVEAEY FNFDSLNFIP DHPARTLQDT FHVGEPGSGQ 190 200 210 220 230 240 VLRTHTSPVQ MRTMLSRDVP VYVVCPGRVF RTDELDATHT PVFHQIEGLA VDKGLTMAHL 250 260 270 280 290 300 KGTLDHLAKT LFGAETKTRM RTNYFPFTEP SAEVDVWFPN KKGGAGWIEW GGCGMVNPNV 310 320 330 340 350 LRAAGIDPEI YNGFAFGMGL ERTLQFRNGL SDMRDMVEGD VRFTLPFGVQ A

References

[1]

"Complete Genome Sequences of Corynebacterium pseudotuberculosis Strains 3/99-5 and 42/02-A, Isolated from Sheep in Scotland and Australia, Respectively." Pethick F.E., Lainson A.F., Yaga R., Flockhart A., Smith D.G., Donachie W., Cerdeira L.T., Silva A., Bol E., Lopes T.S., Barbosa M.S., Pinto A.C., Dos Santos A.R., Soares S.C., Almeida S.S., Guimaraes L.C., Aburjaile F.F., Abreu V.A. , Ribeiro D., Fiaux K.K., Diniz C.A., Barbosa E.G., Pereira U.P., Hassan S.S., Ali A., Bakhtiar S.M., Dorella F.A., Carneiro A.R., Ramos R.T., Rocha F.S., Schneider M.P., Miyoshi A., Azevedo V., Fontaine M.C. J. Bacteriol. 194:4736-4737(2012) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: 42/02-A EMBL AEP70196.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP003062 Genomic DNA. Translation: AEP70196.1.
RefSeq	YP_005692458.1. NC_017306.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEP70196; AEP70196; Cp4202_0938.
GeneID	12852391.
KEGG	cos:Cp4202_0938.
Phylogenomic databases
KO	K01889.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_ligase_II_N. IPR022911. Phe_tRNA_ligase_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G4QYZ1_CORPS

Accession

Primary (citable) accession number: G4QYZ1

Entry history

Integrated into UniProtKB/TrEMBL:	December 14, 2011
Last sequence update:	December 14, 2011
Last modified:	April 3, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)