ID   G4P1Z8_BACS4            Unreviewed;      1228 AA.
AC   G4P1Z8;
DT   14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT   14-DEC-2011, sequence version 1.
DT   27-MAR-2024, entry version 68.
DE   RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE            EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN   OrderedLocusNames=GYO_4108 {ECO:0000313|EMBL:AEP88677.1};
OS   Bacillus spizizenii (strain DSM 15029 / JCM 12233 / NBRC 101239 / NRRL
OS   B-23049 / TU-B-10) (Bacillus subtilis subsp. spizizenii).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1052585 {ECO:0000313|EMBL:AEP88677.1, ECO:0000313|Proteomes:UP000002651};
RN   [1] {ECO:0000313|EMBL:AEP88677.1, ECO:0000313|Proteomes:UP000002651}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15029 / JCM 12233 / NBRC 101239 / NRRL B-23049 / TU-B-10
RC   {ECO:0000313|Proteomes:UP000002651};
RX   PubMed=22493193; DOI=10.1128/JB.05675-11;
RA   Earl A.M., Eppinger M., Fricke W.F., Rosovitz M.J., Rasko D.A.,
RA   Daugherty S., Losick R., Kolter R., Ravel J.;
RT   "Whole-genome sequences of Bacillus subtilis and close relatives.";
RL   J. Bacteriol. 194:2378-2379(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC         Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC         ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00001854};
CC   -!- COFACTOR:
CC       Name=Mo-bis(molybdopterin guanine dinucleotide);
CC         Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC       Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC       {ECO:0000256|ARBA:ARBA00004170}.
CC   -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC       oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
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DR   EMBL; CP002905; AEP88677.1; -; Genomic_DNA.
DR   RefSeq; WP_014115538.1; NC_016047.1.
DR   AlphaFoldDB; G4P1Z8; -.
DR   STRING; 1052585.GYO_4108; -.
DR   KEGG; bst:GYO_4108; -.
DR   HOGENOM; CLU_000422_14_1_9; -.
DR   Proteomes; UP000002651; Chromosome.
DR   GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR   GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR   GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR   CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR   CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR   Gene3D; 3.40.50.12440; -; 1.
DR   Gene3D; 4.10.1200.10; nitrate reductase tail; 1.
DR   InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR   InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR   InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR   InterPro; IPR006656; Mopterin_OxRdtase.
DR   InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR   InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR   InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR   InterPro; IPR006468; NarG.
DR   InterPro; IPR028189; Nitr_red_alph_N.
DR   InterPro; IPR044906; Nitr_red_alph_N_sf.
DR   NCBIfam; TIGR01580; narG; 1.
DR   PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR   PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR   Pfam; PF00384; Molybdopterin; 1.
DR   Pfam; PF01568; Molydop_binding; 1.
DR   Pfam; PF14710; Nitr_red_alph_N; 1.
DR   SMART; SM00926; Molybdop_Fe4S4; 1.
DR   SUPFAM; SSF50692; ADC-like; 1.
DR   SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR   PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR   PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR   PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
DR   PROSITE; PS00932; MOLYBDOPTERIN_PROK_3; 1.
PE   3: Inferred from homology;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Oxidoreductase {ECO:0000313|EMBL:AEP88677.1}.
FT   DOMAIN          47..111
FT                   /note="4Fe-4S Mo/W bis-MGD-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51669"
SQ   SEQUENCE   1228 AA;  139005 MW;  DE75B4ABF6ADE673 CRC64;
     MKKKKMSPLF RRLNYFSPIE HHSNKHSQTT REDRDWENVY RNRWQYDKVV RSTHGVNCTG
     SCSWNIYVKN GIVTWEGQNL NYPSTGPDMP DFEPRGCPRG ASFSWYIYSP LRVKYPYVRG
     VLINLWREAL QAHQNPLDAW KSIVENPEKA KSYKQARGKG GFVRAEWPEV LKLISASLLY
     TVMKYGPDRN VGFSPIPAMS MISHASGSRF MSLIGGPMLS FYDWYADLPP ASPQIWGDQT
     DVPESSDWYN SGYIITWGSN VPLTRTPDAH FLAEARYKGA KVISISPDFA ESSKFADDWL
     SIRQGTDGAL AMAMGHVILQ EFYVNQETER FIEYAKQYTD FPFFVTLSKE NGVYTAGRFL
     HAKDIGRQTK HDQWKPAVWN EQTSAFAIPQ GTMGSRWDGQ QKWNLHMIDE ETGDPIEPRL
     SVLGIEDEIG TVRIPYFSND GNKVLERDLP IKKLNLNGEE VCVTTVFDLI LANYGVNRGF
     GEQSAVSYDD PEPFTPAWQE QMTGIKKEAV IKIAREFAQN AIDTDGRSMI IVGAGINHWF
     NSDTIYRAVL NLVLLVGAQG VNGGGWAHYV GQEKLRPAEG WQTIANAKDW EGVPKLQNGT
     SFFYFATDQW RYEDQPISDL ASPIAASSRY KHHADYNVLA ARLGWLPSYP TFNQNGIDLY
     KEAEKAGATT PEDVGAYVAS QLQEKKLKFA IEDPDNEVNF PRNLFVWRAN LISSSGKGHE
     YFLKHLLGTT NGLMNDDSDS IRPEEINWRE QAPEGKLDLL INLDFRMAGT ALYSDIVLPA
     ATWYEKHDLS STDMHPFIHP FAPAISAPWE SKSDWDIFKA LSKSVSDLAE EVDMEPVKEV
     VATPLLHDTM QELAQPFGKI NDWSRGECEA IPGKTMPNIQ VVERDYKHIF HKMTALGPQA
     GLKPSGTKGM SWSIADEYES LKKRLGEITS DSVAKGCPNI SEAKQAAEAI LTLSSTSNGK
     VAVKAWESLE DITNLKLKDL AEEREEECFT FEQITAQPKT VITSPAFTGS EKGGRRYSPF
     TTNVEKLIPW RTLTGRQSYY VDHELMMEFG ETMATFKPIL QHRPFLSKRP DQEGKEIVLN
     YLTPHNKWSV HSMYFDSLPM LTLFRGGPTV WMNKDDAEDT DIKDNDWIEC FNRNGVVVAR
     AVLSHRIPKG MAFMHHAQDR HINVPGTKLT NNRGGTHNSP TRIHVKPTQM IGGYAQLSYG
     FNYYGPTGNQ RDLNVVIRKL KEVDWLED
//