G4LGB4 (G4LGB4_PSEAI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Alanine racemase HAMAP-Rule MF_01201
EC=5.1.1.1 HAMAP-Rule MF_01201

Gene names

Name:	alr EMBL BAK87480.1
ORF Names:	NCGM2_0592 EMBL BAK87480.1

Organism

Pseudomonas aeruginosa NCGM2.S1 EMBL BAK87480.1

Taxonomic identifier

1089456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity. HAMAP-Rule MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201 RuleBase RU000608 SAAS SAAS020622
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_01201 RuleBase RU000608 SAAS SAAS020622
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201 SAAS SAAS020622
Sequence similarities	Belongs to the alanine racemase family. HAMAP-Rule MF_01201 RuleBase RU004188

Ontologies

Keywords
Ligand	Pyridoxal phosphate HAMAP-Rule MF_01201 RuleBase RU000608 SAAS SAAS020622
Molecular function	Isomerase HAMAP-Rule MF_01201 RuleBase RU000608 SAAS SAAS020622
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	34	1	Proton acceptor; specific for D-alanine By similarity HAMAP-Rule MF_01201
	Active site	254	1	Proton acceptor; specific for L-alanine By similarity HAMAP-Rule MF_01201
	Binding site	130	1	Substrate By similarity HAMAP-Rule MF_01201
	Binding site	302	1	Substrate; via amide nitrogen By similarity HAMAP-Rule MF_01201
Amino acid modifications
	Modified residue	34	1	N6-(pyridoxal phosphate)lysine By similarity HAMAP-Rule MF_01201

Sequences

	Sequence		Length	Mass (Da)
	G4LGB4 [UniParc]. Last modified December 14, 2011. Version 1. Checksum: 2C11524D8BFCEA58	FASTA	363	38,729
	10 20 30 40 50 60 MRPLVATVDL SAIRHNYALA KRCAPQRQAF AVVKANAYGH GAREVVTALH DDADGFAVAC 70 80 90 100 110 120 LEEAAEVRAL HASARILLLE GCFEASEYAL AGQLRLDLVI QGAEQGEAFL AAGLDIPLNV 130 140 150 160 170 180 WLKLDSGMHR LGFDPAALRA WHARLRSHPG VRELNLISHF ACADERNHPL TEQQLESFLG 190 200 210 220 230 240 LLDLDFDQRS LANSAAVLTI PAAHMDWLRP GIMLYGSTPL ADLSAAELGL RPAMSLGAQL 250 260 270 280 290 300 ISLREVAVGE SVGYGATWIA ERPARIGTVS CGYADGYPRT APAGTPVLVG GRRATLAGRV 310 320 330 340 350 360 SMDMLAVDLS DLPEARVGDP VELWGAGLSV DEVARACGTL GYELLSKVGA RVPRAFQYGP GRL

References

[1]	"Complete Genome Sequence of Highly Multidrug-Resistant Pseudomonas aeruginosa NCGM2.S1, a Representative Strain of a Cluster Endemic to Japan." Miyoshi-Akiyama T., Kuwahara T., Tada T., Kitao T., Kirikae T. J. Bacteriol. 193:7010-7010(2011) Cited for: NUCLEOTIDE SEQUENCE. Strain: NCGM2.S1 EMBL BAK87480.1.
[2]	Akiyama T., Kuwahara T., Ando T., Tada T., Kirikae T. Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: NCGM2.S1 EMBL BAK87480.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP012280 Genomic DNA. Translation: BAK87480.1.
RefSeq	YP_005978865.1. NC_017549.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAK87480; BAK87480; NCGM2_0592.
GeneID	12570382.
KEGG	pnc:NCGM2_0592.
Phylogenomic databases
KO	K01775.
Enzyme and pathway databases
BioCyc	PAER1089456:GLIR-592-MONOMER.
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G4LGB4_PSEAI

Accession

Primary (citable) accession number: G4LGB4

Entry history

Integrated into UniProtKB/TrEMBL:	December 14, 2011
Last sequence update:	December 14, 2011
Last modified:	May 29, 2013
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)