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G4LEC9 (G4LEC9_PSEAI) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL BAK92221.1
Synonyms:ddl HAMAP-Rule MF_00047
ORF Names:NCGM2_5400 EMBL BAK92221.1
OrganismPseudomonas aeruginosa NCGM2.S1 [Complete proteome] EMBL BAK92221.1
Taxonomic identifier1089456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain138 – 332195ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding164 – 21754ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2861Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2991Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2991Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3011Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
G4LEC9 [UniParc].

Last modified December 14, 2011. Version 1.
Checksum: A66A99C9B74958E7

FASTA34636,476
        10         20         30         40         50         60 
MGQDKLKVAV LFGGSSEERD VSIASGAQVI QALRSAGHQV LAVDTASGLL GAEEERRLLA 

        70         80         90        100        110        120 
SKVKEVPPDS DSLAIIRSGK QSLLSAGELA GVDVFFLALH GGTGEDGTLQ ALLDAGGFAY 

       130        140        150        160        170        180 
TGSGHLASAM AMDKDVAKRL FLAAGVETAS WLMAPASEEA VREQLGFPLV VKPNSQGSTV 

       190        200        210        220        230        240 
GLSIVHSQAE LQPAIELAGR YGDEVMLERF VAGREVTVGV LDDQALPVGE ILLGGQEVFD 

       250        260        270        280        290        300 
YEHKYQAGAV REVFPADLPP AIAAEAQRLA LKVHRALKLS GYSRTDFRLD EQGRLWCLEV 

       310        320        330        340 
NTLPGMTATS LLPQAAAAAG IGFAELCERI CRLGIERCKG ARKARS 

« Hide

References

[1]"Complete Genome Sequence of Highly Multidrug-Resistant Pseudomonas aeruginosa NCGM2.S1, a Representative Strain of a Cluster Endemic to Japan."
Miyoshi-Akiyama T., Kuwahara T., Tada T., Kitao T., Kirikae T.
J. Bacteriol. 193:7010-7010(2011)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NCGM2.S1 EMBL BAK92221.1.
[2]Akiyama T., Kuwahara T., Ando T., Tada T., Kirikae T.
Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NCGM2.S1 EMBL BAK92221.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP012280 Genomic DNA. Translation: BAK92221.1.
RefSeqYP_005983606.1. NC_017549.1.

3D structure databases

ProteinModelPortalG4LEC9.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAK92221; BAK92221; NCGM2_5400.
GeneID12575190.
KEGGpnc:NCGM2_5400.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycPAER1089456:GLIR-5401-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG4LEC9_PSEAI
AccessionPrimary (citable) accession number: G4LEC9
Entry history
Integrated into UniProtKB/TrEMBL: December 14, 2011
Last sequence update: December 14, 2011
Last modified: June 11, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)