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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

gid

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotationSAAS annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotationSAAS annotation

Cofactori

FADUniRule annotationSAAS annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 126FADUniRule annotation

GO - Molecular functioni

  1. 5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Source: UniProtKB-HAMAP
  2. flavin adenine dinucleotide binding Source: UniProtKB-HAMAP
  3. methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

tRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

FADUniRule annotationSAAS annotation, Flavoprotein

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-760-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:gidImported
Synonyms:trmFOUniRule annotation
ORF Names:THEMA_00985Imported, Tmari_0735Imported
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)Imported
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliG4FD89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

KOiK04094.
OMAiRFGPMKP.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.

Sequencei

Sequence statusi: Complete.

G4FD89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVNVIGAGL AGSEVAYNLG KRGIRVRLFE MRPKKMTEVH KTGYFAELVC
60 70 80 90 100
SNSLKSEDIT NAEGLLKAEM RLMGSITLEA AEKARVPSGK ALAVDRNIFA
110 120 130 140 150
KEVTEVIERL ESVEIIREEV TEFDPEEGIW VVATGPATSD GLLPFLKKLL
160 170 180 190 200
GDDLLFFFDA VSPIVTFESI DMECAFWGDR FGKGKDYINC PLTKEEYEEF
210 220 230 240 250
WKALVEAEVI EMEDFDRKLL FERCQPIEEI ARSGKDALRY GPLRPTGLVD
260 270 280 290 300
PRTGKEPYAV VQLRREDKEG RFYSLVGFQT RLKWSEQKRV LRKIPCLRNA
310 320 330 340 350
EIVRYGVMHR NVYINSPKLL DIFFRLKKHP NIFFAGQITG VEGYMESAAS
360 370 380 390 400
GIYVAYNVHR ILKGLSPLKL PEETMMGALF SYIIEKVEGD LKPMYANFGL
410 420 430
LPPLKVRVKD KFEKRKKLAE RAIETMKKFL EENPW
Length:435
Mass (Da):49,689
Last modified:December 14, 2011 - v1
Checksum:i768BFBAB0C97D57F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP004077 Genomic DNA. Translation: AGL49660.1.
CP007013 Genomic DNA. Translation: AHD17511.1.
RefSeqiNP_228543.1. NC_000853.1.
YP_007977085.1. NC_021214.1.
YP_008990296.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAGL49660; AGL49660; Tmari_0735.
AHD17511; AHD17511; THEMA_00985.
GeneIDi15494338.
18092053.
898401.
KEGGitma:TM0734.
tmi:THEMA_00985.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP004077 Genomic DNA. Translation: AGL49660.1.
CP007013 Genomic DNA. Translation: AHD17511.1.
RefSeqiNP_228543.1. NC_000853.1.
YP_007977085.1. NC_021214.1.
YP_008990296.1. NC_023151.1.

3D structure databases

ProteinModelPortaliG4FD89.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAGL49660; AGL49660; Tmari_0735.
AHD17511; AHD17511; THEMA_00985.
GeneIDi15494338.
18092053.
898401.
KEGGitma:TM0734.
tmi:THEMA_00985.

Phylogenomic databases

KOiK04094.
OMAiRFGPMKP.

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-760-MONOMER.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome organization of Thermotoga maritima reflects its lifestyle."
    Latif H., Lerman J.A., Portnoy V.A., Tarasova Y., Nagarajan H., Schrimpe-Rutledge A.C., Smith R.D., Adkins J.N., Lee D.H., Qiu Y., Zengler K.
    PLoS Genet. 9:E1003485-E1003485(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099Imported and MSB8Imported.
  2. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MSB8Imported.

Entry informationi

Entry nameiG4FD89_THEMA
AccessioniPrimary (citable) accession number: G4FD89
Entry historyi
Integrated into UniProtKB/TrEMBL: December 14, 2011
Last sequence update: December 14, 2011
Last modified: March 4, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.