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Protein

Hydrogen cyanide synthase subunit HcnA

Gene

hcnA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

A three-component membrane-bound flavoenzyme that catalyzes the formation of hydrogen cyanide, a secondary metabolite, by transfer of electrons to a cyanide-resistant branch of the aerobic respiratory chain.4 Publications

Catalytic activityi

Glycine + 2 A = HCN + CO2 + 2 AH2.2 Publications

Enzyme regulationi

Oxygen is necessary for cyanogenesis. Activated by succinate, glycine methyl ester, glucose and D,L-methionine in addition to glycine. Phenazine methosulfate, methylene blue, 2,6-dichlorophenolindophenol (DCIP) and ferricyanide can replace oxygen for the reaction. Inhibited by pyrrolnitrin and acriflavine at 1 mM concentration.2 Publications

Kineticsi

Measured for the whole complex.1 Publication

  1. KM=50 mM for glycine3 Publications

    pH dependencei

    Optimum pH is 8.3 (in the presence of Tris-HCl). Active from 7.3-7.8 in the presence of other buffers.3 Publications

    Temperature dependencei

    Not stable at 0 degrees Celsius. Decrease in activity up to 40% immediately after freeze-drying procedure, with a further decrease up to 10% when stored at -10 degrees Celsius.3 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi60 – 601Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi65 – 651Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi68 – 681Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi81 – 811Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydrogen cyanide synthase subunit HcnAImported (EC:1.4.99.52 Publications)
    Short name:
    HcnA1 Publication
    Alternative name(s):
    Glycine dehydrogenase (cyanide-forming)1 Publication
    Gene namesi
    Name:hcnAImported
    Ordered Locus Names:PA2193
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    Proteomesi
    • UP000002438 Componenti: Chromosome

    Organism-specific databases

    PseudoCAPiPA2193.

    Subcellular locationi

    • Cell membrane 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 104104Hydrogen cyanide synthase subunit HcnAPRO_0000419809Add
    BLAST

    Proteomic databases

    PaxDbiG3XD67.

    Expressioni

    Inductioni

    Reduced oxygen levels.1 Publication

    Interactioni

    Subunit structurei

    Heterotrimer of HcnA, HcnB and HcnC.Curated

    Protein-protein interaction databases

    STRINGi208964.PA2193.

    Structurei

    3D structure databases

    ProteinModelPortaliG3XD67.
    SMRiG3XD67. Positions 21-95.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 97822Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiENOG41065RR. Bacteria.
    COG0446. LUCA.
    HOGENOMiHOG000141947.
    InParanoidiG3XD67.
    KOiK10814.
    OMAiLACQTVC.
    PhylomeDBiG3XD67.

    Family and domain databases

    CDDicd00207. fer2. 1 hit.
    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    [Graphical view]
    SUPFAMiSSF54292. SSF54292. 1 hit.
    PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    G3XD67-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MHLLERQHDI QPLSRADMTI HLNGQPVAAA AGETVLNVLN AVGLRRLARN
    60 70 80 90 100
    DHGQASGAFC GMGVCHCCLV AIDGRPKRRA CQTVVRPGMR VETESNRFDQ

    EERP
    Length:104
    Mass (Da):11,427
    Last modified:November 16, 2011 - v1
    Checksum:iBB640066E8514682
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF208523 Genomic DNA. Translation: AAF21028.1.
    AE004091 Genomic DNA. Translation: AAG05581.1.
    PIRiF83370.
    RefSeqiNP_250883.1. NC_002516.2.
    WP_003113684.1. NZ_ASJY01000328.1.

    Genome annotation databases

    EnsemblBacteriaiAAG05581; AAG05581; PA2193.
    GeneIDi882197.
    KEGGipae:PA2193.
    PATRICi19838834. VBIPseAer58763_2297.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF208523 Genomic DNA. Translation: AAF21028.1.
    AE004091 Genomic DNA. Translation: AAG05581.1.
    PIRiF83370.
    RefSeqiNP_250883.1. NC_002516.2.
    WP_003113684.1. NZ_ASJY01000328.1.

    3D structure databases

    ProteinModelPortaliG3XD67.
    SMRiG3XD67. Positions 21-95.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi208964.PA2193.

    Proteomic databases

    PaxDbiG3XD67.

    Protocols and materials databases

    DNASUi882197.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAG05581; AAG05581; PA2193.
    GeneIDi882197.
    KEGGipae:PA2193.
    PATRICi19838834. VBIPseAer58763_2297.

    Organism-specific databases

    PseudoCAPiPA2193.

    Phylogenomic databases

    eggNOGiENOG41065RR. Bacteria.
    COG0446. LUCA.
    HOGENOMiHOG000141947.
    InParanoidiG3XD67.
    KOiK10814.
    OMAiLACQTVC.
    PhylomeDBiG3XD67.

    Family and domain databases

    CDDicd00207. fer2. 1 hit.
    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    [Graphical view]
    SUPFAMiSSF54292. SSF54292. 1 hit.
    PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHCNA_PSEAE
    AccessioniPrimary (citable) accession number: G3XD67
    Secondary accession number(s): Q7DCD3, Q9REV9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2012
    Last sequence update: November 16, 2011
    Last modified: September 7, 2016
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.