Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Rab11 family-interacting protein 2

Gene

Rab11fip2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity.1 Publication

GO - Molecular functioni

GO - Biological processi

  • establishment of cell polarity Source: MGI
  • insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  • regulated exocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-432040. Vasopressin regulates renal water homeostasis via Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Rab11 family-interacting protein 2
Short name:
Rab11-FIP2
Gene namesi
Name:Rab11fip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1922248. Rab11fip2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Rab11 family-interacting protein 2PRO_0000424063Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei227 – 2271Phosphoserine; by MARK2By similarity

Post-translational modificationi

Phosphorylation at Ser-227 by MARK2 regulates epithelial cell polarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiG3XA57.
MaxQBiG3XA57.
PaxDbiG3XA57.
PRIDEiG3XA57.

PTM databases

iPTMnetiG3XA57.

Expressioni

Gene expression databases

BgeeiQ5HZI0.
ExpressionAtlasiG3XA57. baseline and differential.
GenevisibleiG3XA57. MM.

Interactioni

Subunit structurei

Homooligomerizes in a Rab11-independent manner. Forms a heterooligomeric complex with RAB11FIP4. Interacts with AP2A1, MYO5B, RAB25 and REPS1. Interacts with RAB11A and RAB11B (activated GTP-bound form). Interacts with NPC1L1. Interacts (via NPF motifs) with EHD1 and EHD3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi217140. 2 interactions.
IntActiG3XA57. 1 interaction.
STRINGi10090.ENSMUSP00000059978.

Structurei

3D structure databases

ProteinModelPortaliG3XA57.
SMRiG3XA57. Positions 14-124, 449-502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 102102C2PROSITE-ProRule annotationAdd
BLAST
Domaini437 – 49963FIP-RBDPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 10288Necessary for its cellular translocation to the plasma membraneBy similarityAdd
BLAST
Regioni465 – 51248Necessary for the interaction with AP2A1, RAB11A, subcellular location, endocytosis activity and homooligomerizationBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi323 – 3253NPF 1By similarity
Motifi406 – 4083NPF 2By similarity
Motifi440 – 4423NPF 2By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 FIP-RBD domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IEZZ. Eukaryota.
ENOG410Z26C. LUCA.
GeneTreeiENSGT00530000063203.
HOGENOMiHOG000234700.
HOVERGENiHBG054920.
KOiK12484.
OMAiSFHMNPT.
TreeFamiTF326172.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR019018. Rab-bd_FIP-RBD.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF09457. RBD-FIP. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51511. FIP_RBD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: G3XA57-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMLSEQAQKW FPTHVQVTVL QAKDLKPKGK SGTNDTYTII QLGKEKYSTS
60 70 80 90 100
VAEKTLEPVW KEEASFELPG LLMQGSPEKY ILFLIVMHRS LVGLDKFLGQ
110 120 130 140 150
VAINLNDIFE DKQRRKTEWF RLESKQGKRI KNRGEIKVNI QFMRNNMTAS
160 170 180 190 200
MFDLSMKDKT RSPFAKLKDK MKGRKSDGVF SDTSSAIVPS THMPDANPEF
210 220 230 240 250
SSGEMQMKSK PKKPFLLGPQ RLSSAHSMSD LTGSHLSSEK LKSSTVGPTH
260 270 280 290 300
LLSRQIDSFG VVPESGSLKS PHRRTLSFDT SKLNQPGSIV DEGEHSFGRQ
310 320 330 340 350
SDPFTNVTAS LPQKFATLPR KKNPFEESSE PWDSSMNLFS KPIEVRKESK
360 370 380 390 400
REKREKVSLF ERVTGKRDSR RPDKLNNGGS DSPCDLKSPS AFSENRQDYF
410 420 430 440 450
EYESTNPFTA KFRASTIMPS SSFHVNPTSS EDLRKIPDNN PFDATAGYRS
460 470 480 490 500
LTYEEVLQEL VKHKELLRRK DTHIRELEDY IDNLLVRVME ETPSILRVPY
510
EPSRKAGKFT NS
Length:512
Mass (Da):58,207
Last modified:November 16, 2011 - v1
Checksum:i5A6622C5E0B96728
GO
Isoform 2 (identifier: G3XA57-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     507-512: GKFTNS → VYTQDHLSPGPPHLCGEATSTHS

Show »
Length:529
Mass (Da):59,989
Checksum:iB323D6B7E10F0DA0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei507 – 5126GKFTNS → VYTQDHLSPGPPHLCGEATS THS in isoform 2. 1 PublicationVSP_053310

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC105061 Genomic DNA. No translation available.
AC162855 Genomic DNA. No translation available.
CH466585 Genomic DNA. Translation: EDL01829.1.
BC089010 mRNA. Translation: AAH89010.1.
BC139380 mRNA. Translation: AAI39381.1.
BC139419 mRNA. Translation: AAI39420.1.
CCDSiCCDS38033.1. [G3XA57-2]
RefSeqiNP_001028344.2. NM_001033172.3. [G3XA57-2]
NP_001157839.1. NM_001164367.1.
UniGeneiMm.24167.

Genome annotation databases

EnsembliENSMUST00000051996; ENSMUSP00000059978; ENSMUSG00000040022. [G3XA57-2]
ENSMUST00000170819; ENSMUSP00000133151; ENSMUSG00000040022. [G3XA57-1]
GeneIDi74998.
KEGGimmu:74998.
UCSCiuc008ibm.1. mouse. [G3XA57-1]
uc008ibn.2. mouse. [G3XA57-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC105061 Genomic DNA. No translation available.
AC162855 Genomic DNA. No translation available.
CH466585 Genomic DNA. Translation: EDL01829.1.
BC089010 mRNA. Translation: AAH89010.1.
BC139380 mRNA. Translation: AAI39381.1.
BC139419 mRNA. Translation: AAI39420.1.
CCDSiCCDS38033.1. [G3XA57-2]
RefSeqiNP_001028344.2. NM_001033172.3. [G3XA57-2]
NP_001157839.1. NM_001164367.1.
UniGeneiMm.24167.

3D structure databases

ProteinModelPortaliG3XA57.
SMRiG3XA57. Positions 14-124, 449-502.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217140. 2 interactions.
IntActiG3XA57. 1 interaction.
STRINGi10090.ENSMUSP00000059978.

PTM databases

iPTMnetiG3XA57.

Proteomic databases

EPDiG3XA57.
MaxQBiG3XA57.
PaxDbiG3XA57.
PRIDEiG3XA57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051996; ENSMUSP00000059978; ENSMUSG00000040022. [G3XA57-2]
ENSMUST00000170819; ENSMUSP00000133151; ENSMUSG00000040022. [G3XA57-1]
GeneIDi74998.
KEGGimmu:74998.
UCSCiuc008ibm.1. mouse. [G3XA57-1]
uc008ibn.2. mouse. [G3XA57-2]

Organism-specific databases

CTDi22841.
MGIiMGI:1922248. Rab11fip2.

Phylogenomic databases

eggNOGiENOG410IEZZ. Eukaryota.
ENOG410Z26C. LUCA.
GeneTreeiENSGT00530000063203.
HOGENOMiHOG000234700.
HOVERGENiHBG054920.
KOiK12484.
OMAiSFHMNPT.
TreeFamiTF326172.

Enzyme and pathway databases

ReactomeiR-MMU-432040. Vasopressin regulates renal water homeostasis via Aquaporins.

Miscellaneous databases

ChiTaRSiRab11fip2. mouse.
NextBioi341982.
PROiG3XA57.
SOURCEiSearch...

Gene expression databases

BgeeiQ5HZI0.
ExpressionAtlasiG3XA57. baseline and differential.
GenevisibleiG3XA57. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR019018. Rab-bd_FIP-RBD.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF09457. RBD-FIP. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51511. FIP_RBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Rab11 and its effector Rip11 participate in regulation of insulin granule exocytosis."
    Sugawara K., Shibasaki T., Mizoguchi A., Saito T., Seino S.
    Genes Cells 14:445-456(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN EXOCYTOSIS.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiRFIP2_MOUSE
AccessioniPrimary (citable) accession number: G3XA57
Secondary accession number(s): B9EID4, Q5HZI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: November 16, 2011
Last modified: May 11, 2016
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.